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Glossary for The BioPharm Guide to Fermentation and Cell Culture

genes, removing native genes, or both) through technological means rather than traditional breeding.

genotype The genetic composition of an organism (including expressed and nonexpressed genes), which may not be readily apparent.

germ cell The sex cells in higher animals and plants that carry only half of the organisms genetic material and can combine to develop into new living things.

glycosylation Adding one or more carbohydrate molecules onto a protein (a glycoprotein) after it has been built by the ribosome; a posttranslational modifi cation.

GMPs Good manufacturing practices required by FDA regulations.

Golgi body A cell organelle consisting of stacked membranes where post translational modifications of proteins are performed; also called Golgi appara tus.

growth hormone A protein produced in the pituitary gland to control cell growth.

hemocytometer A device for counting blood cells.

hormone A protein released by an endocrine gland to travel in the blood and act on tissues at another location in the body.

HPLC High-performance liquid chromatography or high-pressure liquid chromatography, a commonly used method for separating liquid mixtures.

hybridoma An immortalized cell line (usually derived by fusing B-lympho cyte cells with myeloma tumor cells) that secretes desirable antibodies.

immortalize To alter cells (either chemically or genetically) so that they can reproduce indefinitely.

inoculate To introduce cells into a culture medium.

inoculum Material (usually cells) introduced i


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