Patricia Powers & Emmanuel Labourier, Ambion, Inc

Quantitative recovery of small RNA such as small interfering RNA (siRNA) and microRNA (miRNA) requires optimized RNA isolation procedures [1]. The mirVana miRNA Isolation Kit is the first commercially available kit specifically designed for efficient recovery of small RNA. Developed for cultured cell lines and mammalian tissue samples, the kit can be used to isolate either total RNA or a fraction enriched in RNA species smaller than 200 nt [1, 2]. The mirVana miRNA Isolation Kit is shown here to be a useful tool for small RNA isolation from plant tissues. Procedures for sample collection, storage, and processing to recover high quality RNA are also discussed.

Plant tissues present some unique challenges for RNA isolation, compared to animal tissues and cells. For example, their cell walls may be very difficult to disrupt, and plant cells often contain significant amounts of compounds such as tannins, phenolics, and complex polysaccharides that can affect RNA quality and inhibit downstream reactions. Ambions mirVana miRNA Isolation Kit includes features that address many of the issues associated with RNA isolation from plant tissue and allow recovery of high quality and high yields of plant RNA.

Recover Intact RNA from Frozen Plant Tissue

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