Navigation Links
Get the 5'-End

Rapid Amplification of cDNA ends (5'-RACE) is a polymerase chain reaction-based technique developed to facilitate the cloning of the 5'-ends of messages (see "Who Needs the 5'-End?"). Ambion's FirstChoice RLM-RACE Kit is a major improvement to the basic RACE protocol. The procedure specifically selects for capped transcripts and can be completed in a single day.


Shortcomings of cDNA Library Synthesis

Most cDNA synthesis reactions performed for cDNA library construction are initiated by oligo (dT) primed reverse transcription. This insures the inclusion of the 3'-end of most mRNA species in the library, and helps to orient the clones during subsequent analysis. However the majority of clones are truncated at their 5'-ends. This is due partly because it is difficult for reverse transcriptase to negotiate long RNA templates and stable secondary structure often encountered in RNA molecules. The predominant method for synthesizing double-stranded cDNA for cloning also contributes to under representation of the 5'-ends of mRNAs in cDNA libraries. Typically, RNase H is used to fragment the mRNA template and the fragments serve as primers for second strand synthesis. (1,2). Statistically, the further towards the 5'-end a sequence lies, the less likely a successful priming event will occur upstream from that end to make the region double-stranded and cloneable. In other words, you get only one chance to prime at the extreme 5'-end of the cDNA and make the 5'-end
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. USB OptiKinase Eliminates Base Bias in Labeling of Oligonucleotide 5-Ends
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Get the End

(Date:9/18/2014)... as aids to weight loss and diabetes prevention, could ... disease; and they do it in a surprising way: ... microbiota the substantial population of bacteria residing in ... mice and humans, were published today in Nature ... the Weizmann Institute,s Immunology Department, who led this research ...
(Date:9/18/2014)... (PRWEB) September 18, 2014 Pipette.com ... include Sartorius/Biohit, Socorex, Capp, and AccuPet brand of ... of 100 uL or less and are a ... forensic applications. , The micropipette promotions were ... and economic alternative products to help customers find ...
(Date:9/17/2014)... 2014 The global consumption of squalene / squalane ... a natural skin-identical chemical that finds applications in various end ... pharmaceuticals, and in other applications like high grade lubrication, and ... few major players and is regionally segregated. Sophim ( ... Co. Ltd. ( Japan ), SeaDragon Marine Oils ...
(Date:9/17/2014)... -- MiMedx Group, Inc. (NASDAQ: MDXG ), ... protected regenerative biomaterials and bioimplants processed from human ... clinical study.    The study article, ... Trial Evaluating the Use of Dehydrated ... Therapy vs. Multi-layer Compression Therapy Alone in the ...
Breaking Biology Technology:Gut bacteria, artificial sweeteners and glucose intolerance 2Gut bacteria, artificial sweeteners and glucose intolerance 3Major Pipette Distributor Pipette.com Announces Promotions on Sartorius/Biohit, Socorex, Capp, and AccuPet Micropipettes 2Major Pipette Distributor Pipette.com Announces Promotions on Sartorius/Biohit, Socorex, Capp, and AccuPet Micropipettes 3Squalene Market by Source (Animal & Vegetable) & by End-user Industry (Cosmetics, Pharmaceuticals & Food Industry) - Global Trends & Forecasts to 2019 2Squalene Market by Source (Animal & Vegetable) & by End-user Industry (Cosmetics, Pharmaceuticals & Food Industry) - Global Trends & Forecasts to 2019 3MiMedx Multi-Center Randomized, Controlled VLU Clinical Study Published In Peer-Reviewed Journal 2MiMedx Multi-Center Randomized, Controlled VLU Clinical Study Published In Peer-Reviewed Journal 3MiMedx Multi-Center Randomized, Controlled VLU Clinical Study Published In Peer-Reviewed Journal 4
... , MISSISSAUGA, ON, Nov. 11 /PRNewswire-FirstCall/ - YM BioSciences ... product development company that identifies and advances a diverse ... development, today reported operational and financial results for the ... , "During our first fiscal quarter, we continued to ...
... Nov. 11 /PRNewswire-Asia-FirstCall/ -- China Sky One,Medical, Inc. ("China ... ), a,leading fully integrated pharmaceutical company producing over-the-counter,drugs in ... it will,conduct a conference call at 8:00 a.m. Eastern ... its third quarter 2009 financial results. , ...
... Particle Sciences Inc. , a leading pharmaceutical CRO, has ... bringing their total to approximately 21,000 sq ft. "Our ... Dr. Mark Mitchnick, CEO. "2009 has been a record ... the next 12 months. The additional space will be ...
Cached Biology Technology:YM BIOSCIENCES REPORTS FISCAL FIRST QUARTER 2010 OPERATIONAL AND FINANCIAL RESULTS 2YM BIOSCIENCES REPORTS FISCAL FIRST QUARTER 2010 OPERATIONAL AND FINANCIAL RESULTS 3YM BIOSCIENCES REPORTS FISCAL FIRST QUARTER 2010 OPERATIONAL AND FINANCIAL RESULTS 4YM BIOSCIENCES REPORTS FISCAL FIRST QUARTER 2010 OPERATIONAL AND FINANCIAL RESULTS 5YM BIOSCIENCES REPORTS FISCAL FIRST QUARTER 2010 OPERATIONAL AND FINANCIAL RESULTS 6YM BIOSCIENCES REPORTS FISCAL FIRST QUARTER 2010 OPERATIONAL AND FINANCIAL RESULTS 7YM BIOSCIENCES REPORTS FISCAL FIRST QUARTER 2010 OPERATIONAL AND FINANCIAL RESULTS 8YM BIOSCIENCES REPORTS FISCAL FIRST QUARTER 2010 OPERATIONAL AND FINANCIAL RESULTS 9YM BIOSCIENCES REPORTS FISCAL FIRST QUARTER 2010 OPERATIONAL AND FINANCIAL RESULTS 10YM BIOSCIENCES REPORTS FISCAL FIRST QUARTER 2010 OPERATIONAL AND FINANCIAL RESULTS 11YM BIOSCIENCES REPORTS FISCAL FIRST QUARTER 2010 OPERATIONAL AND FINANCIAL RESULTS 12China Sky One Medical, Inc. Announces Conference Call to Discuss Third Quarter 2009 Results 2China Sky One Medical, Inc. Announces Conference Call to Discuss Third Quarter 2009 Results 3Particle Sciences Acquires Additional Space for Operations 2
(Date:9/17/2014)... the Copenhagen Centre for Social Evolution and Yale University ... birth weight and -length can partially predict the likelihood ... autism and schizophrenia later in life. The study analyzed ... hospital diagnoses for up to 30 years, and adjusted ... is published today in the Proceedings of the ...
(Date:9/17/2014)... at UTSouthwestern Medical Center have found that the most ... apoE3, helps repair the lining of blood vessels. Individuals ... benefit of this repair, putting them at higher risk ... identified one mechanism by which apoE3 promotes a healthy ... detrimental," said Dr. Philip Shaul , Professor of ...
(Date:9/17/2014)... complex social behavior by studying nonhuman mammals and primates, ... With their unusually large brains relative to their body ... social environment---not merely in a large population of cooperating ... dynamic setting of alliances and competitors. The same is ... and social carnivores, like hyenas and lions. , A ...
Breaking Biology News(10 mins):Size at birth affects risk of adolescent mental health disorders 2Size at birth affects risk of adolescent mental health disorders 3Protein variant may boost cardiovascular risk by hindering blood vessel repair 2Being social: Learning from the behavior of birds 2
... and German . Researchers at ... Switzerland have developed a novel method for producing dark-field ... industrial imaging equipment. Dark-field images provide more detail ... diagnose the onset of osteoporosis, breast cancer or Alzheimers ...
... In the January 18th issue of Molecular Cell, Case ... Baker, Ph.D. challenges molecular biologys established body of evidence ... decay. With her collaborator, Ambro van Hoof, Ph.D. of ... tested the faux 3 UTR model and proved it ...
... National Academy of Sciences, Sackler Colloquium series will ... These networks represent the genomic program for the ... types. The meeting will focus on four ... embryonic development; gene networks later developmental processes; regulatory ...
Cached Biology News:New technology sharpens X-ray vision 2Case researcher in RNA biology makes waves by challenging current thinking 2
... Hybrid-Ready Tissues are selected tissues from ... been fixed, embedded in paraffin, sectioned and ... the slides are ready for use in ... The Hybrid-Ready tissue slides are suitable for ...
...
Quantibody Human Angiogenesis Array 1 (1 slide) Class: Quantitative Antibody Arrays Product Group: Antibody Array...
Immunochemistry 1 & 2...
Biology Products: