Navigation Links
Genotyping Single Nucleotide Polymorphisms with Molecular Beacons

STD=31.00.44). The different Ct values observed for the two heterozygous individuals were likely caused by a difference in DNA concentration between the samples.

From the distribution of data, the three genotypes were easily distinguished from one another with either of the two molecular beacons. The advantage of having both molecular beacons is to clearly differentiate a homozygote from a heterozygote. For example, one heterozygote had Cts of approximately 31 with both molecular beacons, and the Ct values were not dramatically different from the complementary homozygous targets A/A of 29 Ct with probe A or G/G of 28 Ct with probe G. The perfectly matched homozygotes (A/A or G/G) became mismatched with the other probe (G or A), which resulted in a Ct of 40, so it was easily distinguished from heterozygous target (Ct=31). Negative control samples (shown as black closed dots) containing no template also generated no fluorescence (Ct=40). We blind-tested more samples and obtained very similar results with clear discrimination among different genotypes (data not shown). Results obtained from using molecular beacons were in complete agreement with results generated by conventional sequencing methods.

Conclusions

Stratagenes custom-designed molecular beacons are ideal for single nucleotide polymorphism genotyping. SNP genotyping requires only prior knowledge of a single nucleotide change within a target sequence and the design of a corresponding molecular beacon. With these requirements met, the subsequent molecular beacon procedure takes a quick 2 hours and is easily carried out in a 96-well format for large-scale screening. Additionally, using different molecular beacons labeled with different fluorophores to target different SNPs should make it possible to detect multiple polymorphisms5 simultaneously
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System
2. Purified Single-Stranded M13 DNA for Automated Sequencing
3. Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon Allelic Discrimination Kits
4. XL10-Gold Cells Supplied in New Single-Reaction Format
5. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
6. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
7. RNA, DNA, and Protein from a Single Sample
8. PFGE Analysis of Chromosomal DNA From a Single Hair Root
9. Single-Cell Complementation of Barley Mlo Mutants Using a PDS-1000/He Hepta System
10. Transient Gene Expression Following Single and Double Exponential Decay Electroporation Pulses
11. Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method
Post Your Comments:
(Date:7/25/2014)... At the request of the AMA, ... extension for the public comment period (Docket No. FAA-2014-0396) ... Aircraft established by Congress as part of the FAA ... establishes the new deadline for comments as September 23, ... notice published in the Federal Register on July 25, ...
(Date:7/24/2014)... 2014  Now available is a stem cell therapy ... Therapies , located in Guadalajara, Mexico ... a new health option: stem cell therapies for ... patients suffer from joint and muscle injuries due to ... torn rotator cuff, tennis elbow, and knee injuries. Throughout ...
(Date:7/24/2014)... computers have yet to materialise. Yet, scientists are ... such computers faster. One such approach relies on ... by applying an electric field. A new study ... coupled quantum dots (TQDs) with electrical impulses can ... example, should TQDs be used as quantum information ...
(Date:7/24/2014)... July 24, 2014 Your cell ... in modeling lithium-ion battery storage capacity. , New ... a theoretical model created at Lawrence Livermore ... that predicts how carbon components will perform as ... emphasizes the urgent need for higher-performance batteries. Several ...
Breaking Biology Technology:FAA Grants Comment Extension at the Request of AMA 2Stem Cells In Sports Injuries, Joints And Muscular Pain 2Stem Cells In Sports Injuries, Joints And Muscular Pain 3Getting More Life out of Lithium-ion Batteries 2
... Spanish . , Scientists at the University ... time different antioxidant compounds from foods such as olive oil, ... have used two new techniques, capillary electrophoresis and high resolution ... a great part of the phenolic compounds contained in these ...
... Dec. 8 Transgenomic, Inc. (OTC Bulletin Board: TBIO) announced ... first in a family of mutation detection kits the company ... ® HS DHPLC platform. SURVEYOR Scan technology is ... amplification reaction and hybridization to form heteroduplex DNA molecules in ...
... Dec. 8 PharmAthene, Inc. (NYSE Amex: PIP ) ... medical countermeasures against chemical and biological threats, announced today that ... Time, on Wednesday, December 9, 2009 to provide an update ... The dial-in number for U.S. callers is 866-383-8108 and for ...
Cached Biology Technology:New antioxidant compounds have been identified in foods such as olive oil, honey and nuts 2Transgenomic Announces the Commercial Launch of SURVEYOR(R) Scan K-RAS Mutation Detection Kit 2Transgenomic Announces the Commercial Launch of SURVEYOR(R) Scan K-RAS Mutation Detection Kit 3Transgenomic Announces the Commercial Launch of SURVEYOR(R) Scan K-RAS Mutation Detection Kit 4PharmAthene Announces Updated Time for Conference Call to Review BARDA rPA Decision 2
(Date:7/25/2014)... The routine use of a molecular testing panel ... performing the correct initial surgery for patients with ... University of Pittsburgh Cancer Institute (UPCI), partner with ... Multidisciplinary Thyroid Center and other diagnostic testing agencies, ... initial surgery by 30 percent, according to the ...
(Date:7/24/2014)... loss leads to conflict among people around the world, ... (WCS) Health & Ecosystems: Analysis of Linkages (HEAL) Program ... to tackle global biodiversity decline. , The harvest of ... people and provides protein for more than a billion ... surprise that today,s unprecedented loss of wildlife, is bringing ...
(Date:7/24/2014)... use of native animal species for food or commercial ... significant factor in the decline of many species of ... indicates that more than half of the species being ... hornbills., "By surveying not only the meat made available ... inside the forest by hunters and brought to villages ...
Breaking Biology News(10 mins):Test increases odds of correct surgery for thyroid cancer patients 2Test increases odds of correct surgery for thyroid cancer patients 3New study draws links between wildlife loss and social conflicts 2
... 2013 As an expert in molecular genetics, Colin E. ... Medical Center,s Institute for Regenerative Medicine is to apply the ... human organs. But, with a background specifically in reproductive ... the over-population of homeless dogs and cats. Bishop has been ...
... provides the same quality of fuel for athletes as a ... nutrients that are more commonly found in animal products, according ... (IFT) Annual Meeting & Expo. The research was compiled ... He was unable to attend the meeting, so his presentation ...
... Health has awarded Virginia Commonwealth University a grant totaling ... work which may one day point researchers to ... inflammatory conditions such as rheumatoid arthritis, multiple sclerosis and ... by John Ryan, Ph.D., associate professor in the Department ...
Cached Biology News:Low-cost sterilization method for cats and dogs is focus of new research project 2Monitoring nutrient intake can help vegetarian athletes stay competitive 2VCU receives NIH grant to examine the biology of allergic disease 2
...
...
Kit for testing the ability of a compound to simulate lipolysis in cultured human adipocytes. Kit contains controls, buffers and reagents for detecting glycerol and non-esterified free fatty acids. A...
The strip cap tool is a used for properly seating 8- and 12-cap strips in microplates or strip tubes. A grooved channel on one side is designed to seat domed caps, while the flat edge on the opposite...
Biology Products: