Navigation Links
Genotyping Single Nucleotide Polymorphisms with Molecular Beacons

STD=31.00.44). The different Ct values observed for the two heterozygous individuals were likely caused by a difference in DNA concentration between the samples.

From the distribution of data, the three genotypes were easily distinguished from one another with either of the two molecular beacons. The advantage of having both molecular beacons is to clearly differentiate a homozygote from a heterozygote. For example, one heterozygote had Cts of approximately 31 with both molecular beacons, and the Ct values were not dramatically different from the complementary homozygous targets A/A of 29 Ct with probe A or G/G of 28 Ct with probe G. The perfectly matched homozygotes (A/A or G/G) became mismatched with the other probe (G or A), which resulted in a Ct of 40, so it was easily distinguished from heterozygous target (Ct=31). Negative control samples (shown as black closed dots) containing no template also generated no fluorescence (Ct=40). We blind-tested more samples and obtained very similar results with clear discrimination among different genotypes (data not shown). Results obtained from using molecular beacons were in complete agreement with results generated by conventional sequencing methods.

Conclusions

Stratagenes custom-designed molecular beacons are ideal for single nucleotide polymorphism genotyping. SNP genotyping requires only prior knowledge of a single nucleotide change within a target sequence and the design of a corresponding molecular beacon. With these requirements met, the subsequent molecular beacon procedure takes a quick 2 hours and is easily carried out in a 96-well format for large-scale screening. Additionally, using different molecular beacons labeled with different fluorophores to target different SNPs should make it possible to detect multiple polymorphisms5 simultaneously
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System
2. Purified Single-Stranded M13 DNA for Automated Sequencing
3. Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon Allelic Discrimination Kits
4. XL10-Gold Cells Supplied in New Single-Reaction Format
5. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
6. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
7. RNA, DNA, and Protein from a Single Sample
8. PFGE Analysis of Chromosomal DNA From a Single Hair Root
9. Single-Cell Complementation of Barley Mlo Mutants Using a PDS-1000/He Hepta System
10. Transient Gene Expression Following Single and Double Exponential Decay Electroporation Pulses
11. Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method
Post Your Comments:
(Date:12/15/2014)... , Dec. 15, 2014  GlassesOff Inc. (OTCBB: GLSO) ... as an independent director of the Company,s Board ... AeroScout and served as its CEO until its acquisition ... a $240 million transaction. Recognized as the inventor of ... leadership, AeroScout provided Wi-Fi -based RFID solutions focused on ...
(Date:12/15/2014)... , Dec. 15, 2014  Ascendis Pharma A/S, ... technology to address significant unmet medical needs, today ... Phase 2 pediatric study to evaluate once-weekly TransCon ... or GHD.  This interim analysis consists of 25 ... enrollment in the study, completing all six months ...
(Date:12/13/2014)... and Virgin Unite Canada, the non-profit foundation of the Virgin ... Canadian entrepreneurs tackling social and environmental challenges. Today, Sir ... Centre to announce the new partnership, which includes the development ... $1 million in seed capital provided by Virgin Unite Canada ... Foundation, founded by Alison Lawton . ...
(Date:12/13/2014)... The DNA Microarray market market was valued at ... grow at a CAGR of 15.2% from 2014 ... the DNA Microarray for an in-depth analysis of ... various tables and figures. , http://www.micromarketmonitor.com/market-report/dna-microarray-reports-5177428198.html ... instrument, consumables, and services, where the consumables market ...
Breaking Biology Technology:AeroScout Founder Joins GlassesOff's Board of Directors 2AeroScout Founder Joins GlassesOff's Board of Directors 3Ascendis Pharma A/S Announces Positive Six-Month Interim Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 2Ascendis Pharma A/S Announces Positive Six-Month Interim Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 3Ascendis Pharma A/S Announces Positive Six-Month Interim Results from a Phase 2 Pediatric Study of Once-Weekly TransCon Growth Hormone for the Treatment of Growth Hormone Deficiency 4MaRS and Virgin Unite partner to invest in business as a force for good 2MaRS and Virgin Unite partner to invest in business as a force for good 3DNA Microarray Market Is Expected to Grow at a CAGR of 15.2% from 2014 to 2019 - A Report by MicroMarket Monitor 2DNA Microarray Market Is Expected to Grow at a CAGR of 15.2% from 2014 to 2019 - A Report by MicroMarket Monitor 3DNA Microarray Market Is Expected to Grow at a CAGR of 15.2% from 2014 to 2019 - A Report by MicroMarket Monitor 4
... - No Incidences of Nonconformance Found for Last ... ... ... Sept. 6 Carrington,Laboratories, Inc. (Pink Sheets: CARN) today announced that its bulk,pharmaceutical manufacturing ...
... Generic Industry Readiness and Questions Fiscal ... of Delaying Biogenerics -, WASHINGTON, Sept. 6 ... decades-old patents,expire, and generic companies stand ready to add ... pass legislation enabling the U.S.,Food and Drug Administration (FDA) ...
... Boston Scientific,Corporation (NYSE: BSX ) today announced that ... a Director. Mr. Elliott, 58, is Chairman of ... Chairman, President and Chief Executive Officer of,Zimmer from 2001 ... Elliott,has extensive operating and director experience in medical devices,orthopaedics ...
Cached Biology Technology:Carrington Reports on ISO 9001:2000 Inspection at Sabila Industrial in Costa Rica 2Carrington Reports on ISO 9001:2000 Inspection at Sabila Industrial in Costa Rica 3Biogenerics Will Save Billions, Says Hospira CEO 2Biogenerics Will Save Billions, Says Hospira CEO 3Biogenerics Will Save Billions, Says Hospira CEO 4Boston Scientific Announces Election of Ray Elliott to Its Board of Directors 2Boston Scientific Announces Election of Ray Elliott to Its Board of Directors 3
(Date:12/17/2014)... DUBLIN , Dec. 15, 2014 Research ... announced the addition of the "Samsung Galaxy ... Analysis" report to their offering. ... on a totally different sensing technology than the ... a fingerprint sensor in its product. ...
(Date:12/17/2014)... Dec. 16, 2014 Research and Markets ... of the "Global Chemical Sensor Market 2015-2019" ... http://photos.prnewswire.com/prnh/20130307/600769 One major ... demand for medical sensors in biomedical applications. Chemical ... quick and correct diagnosis during surgical procedures. The ...
(Date:12/11/2014)... 09, 2014 Research and Markets ... ( http://www.researchandmarkets.com/research/9ql3kr/biometrics_market ) has announced the addition ... report to their offering. ... the adoption of multimodal biometric systems. Multimodal biometric ... individual for verification and identification purposes. This helps ...
Breaking Biology News(10 mins):Samsung Galaxy S5 - Home Button Synaptics Fingerprint Sensor Technology Analysis 2Global Chemical Sensor Market 2015-2019: Key Vendors are Abbott Laboratories, Bayer, Hoffmann La-Roche, Johnson & Johnson, NGK Spark Plugs and Robert Bosch 2Biometrics Market in India 2015-2019: Key Vendors are 3M Cogent, NEC, Safran and Suprema 2
... Children,s National Medical Center have discovered that ... are significantly more likely to have low levels of ... This study supports recent research that suggests vitamin D ... keeping bones healthy. Vitamin D deficiency has been recently ...
... March 17 Orbel,s innovative Board Shield ... PCB shield. Visit the BLS section   ... variety of surface mount or through-hole configurations. Then click ... lets you enter shield dimensions, mounting style, and ...
... In the 1930s, soil used as ballast to weigh down ... red imported fire ant to the southern United States. Since ... Maryland and as far west as California, shorting out streetlights ... Plant pests like the fire ant cost the U.S. ...
Cached Biology News:Study: Low levels of vitamin D linked to higher rates of asthma in African-American kids 2Orbel Introduces the Board Shielding Wizard 2From international harbor to native habitat 2From international harbor to native habitat 3