Navigation Links
Genotyping Single Nucleotide Polymorphisms with Molecular Beacons

tion, it adopts a hairpin structure that brings the fluorphore and quencher sufficiently close to each other to allow efficient quenching of the fluorophore. If, however, the molecular beacon is bound to its complementary target, the fluorophore and quencher are far enough apart that the fluorophore cannot be quenched and the molecular beacon fluoresces.

Custom-Designed Molecular Beacons

Hybridization of molecular beacons with their complementary target is highly specific; therefore, we designed molecular beacons to study one of the SNPs of our interest. Based on linkage and association studies in a large Arab family with 20 affected relatives, a diabetes susceptibility locus (IDDM17) maps to 10q25.1.4 IDDM 17 and its flanking markers are localized to a 120-kb region within a completely sequenced bacterial artificial chromosome (BAC) based on association studies of a number of SNPs. We genotyped a group of people in the family and determined the presence of three genotypes. The genotypes were complementary with probe-target homozygote (A/A), mismatched probe-target homozygote (G/G), and heterozygote (A/G) for the SNP.

The fluorescence of the molecular beacon was monitored in real time while the PCR reaction was taking place. The fluorescent signal was monitored and reported during each anneal-extend step when the molecular beacon was bound to its complementary target. Results were displayed on an amplification plot, which reflected the change in fluorescence during cycling. Figure 1 shows the dissociation curves of hybrids between molecular beacons and A/A homozygote (green dots) or G/G homozygote (black circles). Fluorescence was plotted as the derivative value on the Y-axis. In the temperature interval from 62C to 72C, the perfectly complementary probe-target hybrid
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Rapid SNP Genotyping of the Pharmacogenomically Important Allele CYP2D6*4 Using Real-Time qPCR on the DNA Engine Opticon 2 System
2. Purified Single-Stranded M13 DNA for Automated Sequencing
3. Detect Single Nucleotide Sequence Changes with Mx4000 Molecular Beacon Allelic Discrimination Kits
4. XL10-Gold Cells Supplied in New Single-Reaction Format
5. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
6. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
7. RNA, DNA, and Protein from a Single Sample
8. PFGE Analysis of Chromosomal DNA From a Single Hair Root
9. Single-Cell Complementation of Barley Mlo Mutants Using a PDS-1000/He Hepta System
10. Transient Gene Expression Following Single and Double Exponential Decay Electroporation Pulses
11. Simultaneous Detection of CYP3A4, CYP2D6 and CYP2C9 Metabolites with a Single, Sensitive, LC/MS/MS Method
Post Your Comments:
(Date:7/28/2014)... PA & Boston, MA (PRWEB) July 28, 2014 ... collaboration agreements with two researchers to further develop potential ... deadly malignant brain tumor, in combination with their patented ... by ITI and Duke University, are based on the ... Chief, Division of Neurosurgery at The Preston Robert Tisch ...
(Date:7/28/2014)... DC (PRWEB) July 28, 2014 It’s a ... GI Bill benefits to further their post-secondary education are failing ... a study released this year. And according to an analysis ... this shortfall is veteran students’ uninformed choice of college majors, ... The details of the study: Some 51.7 percent of post-9/11 ...
(Date:7/28/2014)... STATION, Texas , July 28, 2014   The ... a 177-key, 4-star boutique hotel in the heart of The Atlas ... Photo - http://photos.prnewswire.com/prnh/20140725/130455 Photo ... within ATLAS , The Atlas ... rooms and suites, a sleek restaurant and bar, a wellness spa, ...
(Date:7/26/2014)... NY (PRWEB) July 26, 2014 ... provides key market data on the Canada Endoscopy ... millions of US dollars, volume (in units) and ... - High-End Endoscopy Visualization Systems, Mid-Range Endoscopy Visualization ... Full Report at http://www.marketresearchreports.biz/analysis/213254 . , The ...
Breaking Biology Technology:String of Collaborations Launch Immunomic Therapeutics’ Brain Tumor Program Pipeline 2String of Collaborations Launch Immunomic Therapeutics’ Brain Tumor Program Pipeline 3String of Collaborations Launch Immunomic Therapeutics’ Brain Tumor Program Pipeline 4Resume Place: To Choose the Best College Majors, Veterans Need Early Career Counseling, Data Shows 2Resume Place: To Choose the Best College Majors, Veterans Need Early Career Counseling, Data Shows 3Resume Place: To Choose the Best College Majors, Veterans Need Early Career Counseling, Data Shows 4Texas' Biocorridor Announces the Atlas Hotel, Centerpiece of Mixed Use Community for Biotech Industry 2Texas' Biocorridor Announces the Atlas Hotel, Centerpiece of Mixed Use Community for Biotech Industry 3Texas' Biocorridor Announces the Atlas Hotel, Centerpiece of Mixed Use Community for Biotech Industry 4Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 2Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 3Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 4Canada Endoscopy Visualization Systems Market Outlook to 2020: MarketResearchReports.Biz 5
... today announced the receipt of a two-year Small Business ... the National Institute on Aging, National Institutes of Health ... antibodies targeting tau protein oligomers in the development of ... be screened at the Michigan High Throughput Screening Center ...
... Wound Management Technologies, Inc., (OTC Bulletin Board: ... solutions, announced today that its President, Deborah Jenkins Hutchinson, ... product, CellerateRX®, recent strategic developments, and its product pipeline ... be held at the JW Marriott Marquis Miami on ...
... a way to overcome one of the main limitations of ... Everyone who has had an ultrasound, including most pregnant women, ... of the limits to the detail obtainable with sonography is ... dictate that the smallest objects you can "see" are about ...
Cached Biology Technology:Oligomerix, Inc. Awarded Phase II NIH Grant to Discover Novel Drugs for Alzheimer's Disease 2Wound Management Technologies to Present at LifeTech Capital Miami Medical Investors Conference 2Novel metamaterial vastly improves quality of ultrasound imaging 2
(Date:7/27/2014)... In one of the largest prevalence studies to date, ... genotype prevalence estimates for the hepatitis C virus (HCV). ... Association for the Study of Liver Diseases, indicate that ... 83 million patients infected of which one-third reside in ... cases, is the next most prevalent, followed by genotypes ...
(Date:7/27/2014)... RESEARCH UK scientists at Barts Cancer Institute have found ... cancer therapy significantly more effective, according to research published ... Barts Cancer Institute, part of Queen Mary University of ... kinase (FAK), signals the body to repair itself after ... DNA. When the researchers removed FAK from blood vessels ...
(Date:7/25/2014)... Before the colonial era, 100,000s of people lived on ... cultures manipulated fire to control the availability of plants ... tribes continue to use fire to maintain desired habitat ... the U.S. Forest Service,s Pacific Southwest Station, will lead ... National Wildfire Refuge during the Ecological Society of America,s ...
Breaking Biology News(10 mins):Hepatitis C virus genotype 1 is most prevalent worldwide 2New drug target can break down cancer's barrier against treatment 2Fire ecology manipulation by California native cultures 2Fire ecology manipulation by California native cultures 3
... the University of Maryland Center for Environmental Science ... a lack of predators and competitors was the ... dogfish observed in Southern New Englands Georges Bank ... 1980s. In an article appearing in the ...
... a new environment while others do not" Scientists from ... headway on this question after analyzing how fast cane ... 70 years of data on toad presences collected since ... regions of Australia at dramatically divergent rates. These variable ...
... Arenas, Chile, on the tip of South America, to spend ... Southern Ocean. Here they hope to make groundbreaking measurements to ... and sea, and vice-versa. The cruise, ... the greenhouse gas carbon dioxide moves between the ocean and ...
Cached Biology News:Researchers offer new theory for dogfish and skate population outburst on George's Bank 2Voyage to Southern Ocean aims to study air-sea fluxes of greenhouse gases 2