Navigation Links
Genomic DNA Preparation from RNAlater Preserved Tissues


The following protocol is suitable for 100 mg tissue samples. Mouse kidney, lung, and spleen genomic DNA have been successfully extracted with approximate yields of 70 g DNA per 100 mg tissue.

Required Materials

Razor blade or scissors 55C incubator
Nuclease-free 2.0 ml microfuge tubes Nuclease-free water
1 M Tris, pH 8.0 Buffer saturated phenol
0.5 M EDTA Chloroform
10% SDS 3 M Sodium acetate, pH 5.2
Proteinase K 95% Ethanol


Protocol

  1. Prepare digestion buffer:
    60 mM Tris, pH 8.0
    100 mM EDTA
    0.5% SDS

  2. Remove tissue from RNAlater and mince well. Cutting the tissue into very small pieces is absolutely essential to achieving adequate digestion. Place in a 2.0 ml eppendorf tube with 1.5 ml digestion buffer.

  3. Add Proteinase K to a final concentration of 500 g/ml. Mix by inversion. Incubate the sample at 55C for approximately 4 hours
    '"/>


Source:


Page: All 1 2 3

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. New Protocols for Isolating High- Molecular-Weight Genomic DNA
3. Easily Amplify Genomic DNA with Long-Distance PCR
4. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
5. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
6. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
7. Mouse Tail Genomic DNA Isolation Protocol(1)
8. Genomic DNA Isolation Protocol(1,2,3)
9. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
10. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
11. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Genomic DNA Preparation from RNAlater Preserved Tissues

(Date:8/31/2015)... ... 31, 2015 , ... There’s a new treatment option for excessive menstrual bleeding ... new FDA approved system in the last 14 years, and the only endometrial ablation ... bleeding) rate of 66%, the high Patient Satisfaction rate of 98%, and the fastest ...
(Date:8/28/2015)... -- Research and Markets ( http://www.researchandmarkets.com/research/zsgg5n/global_and_usa ... & USA BioSimilar Market Analysis to 2021" report ... Biosimilar market will be initiated in September as Sandoz ... the global market react? Will biosimilars explode into the ... the US economy up to $250 billion within the ...
(Date:8/28/2015)... Pittsburgh, PA (PRWEB) , ... August 28, 2015 ... ... Program Chairman, Chuck Gardner, has organized and will present a session on chemical ... September 2. This conference and exposition is Asia’s largest analytical and scientific instruments ...
(Date:8/28/2015)... ... August 28, 2015 , ... Since the initial launch of FireflySci Inc. (FFS) in early 2015, ... customer service. Images such as the Fonz in a lab coat and Large Marge ... the humdrum spectroscopy field. , FireflySci is proud to be the only cuvette ...
Breaking Biology Technology:U.S. FDA Approves Minerva Endometrial Ablation System 2U.S. FDA Approves Minerva Endometrial Ablation System 3Global & USA BioSimilar Market Analysis Report 2015-2021 2Global & USA BioSimilar Market Analysis Report 2015-2021 3U.S. Symposium on Imaging Organized by Pittcon’s Program Chairman Chuck Gardner 2U.S. Symposium on Imaging Organized by Pittcon’s Program Chairman Chuck Gardner 3Cuvette Manufacturer FireflySci Launches Back to School Deals with Complimentary Shipping On All Orders 2
... Incorporated (Nasdaq: SPEX ), an innovator in ... and regulatory consulting services to food, supplement, biotechnology and ... the 2009 BIO International Conference in Atlanta, Georgia on ... Control Drug" booth will showcase their ongoing global phase ...
... Biomarkers and Non-Invasive Imaging to Demonstrate Mechanism of Action ... May 14 VIA Pharmaceuticals, Inc. (Nasdaq: ... of compounds for the treatment of cardiovascular and metabolic ... a Phase 2 clinical trial of its lead drug, ...
... manufacturing facility opens with substantially increased capacity, ... Biosystems Inc. (TSX:MBX) announced today robust double digit growth ... for the second quarter ended March 31, 2009. , ... of Microbix said "Our 43% growth in the first ...
Cached Biology Technology:Spherix to Exhibit at the BIO 2009 International Convention 2VIA Pharmaceuticals Announces Complete Enrollment in FDG-PET Phase 2 Study of VIA-2291 in Cardiovascular Patients 2VIA Pharmaceuticals Announces Complete Enrollment in FDG-PET Phase 2 Study of VIA-2291 in Cardiovascular Patients 3VIA Pharmaceuticals Announces Complete Enrollment in FDG-PET Phase 2 Study of VIA-2291 in Cardiovascular Patients 4VIA Pharmaceuticals Announces Complete Enrollment in FDG-PET Phase 2 Study of VIA-2291 in Cardiovascular Patients 5Microbix Sales Grow Over 30% In The Second Quarter; Up 43% In The First Half '09 2Microbix Sales Grow Over 30% In The Second Quarter; Up 43% In The First Half '09 3Microbix Sales Grow Over 30% In The Second Quarter; Up 43% In The First Half '09 4
(Date:8/25/2015)... and BELLEVUE, Wash. , Aug. ... advanced robotic systems, announced today it will unveil its ... vehicle (UGV), at the National Tactical Officers Association Conference ... The Guardian S is the first-ever commercially available ... years of research and in-field trials and is protected ...
(Date:8/20/2015)... CT ., Aug. 20, 2015 NXT-ID, ... a biometric authentication company focused on the growing mobile ... announces it has joined The Smart Card Alliance and ... Smart Card Alliance and the Smart Card Alliance Latin ... visibility to industry peers and thought leaders promoting adoption ...
(Date:8/12/2015)... , Aug. 12, 2015   MedNet Solutions ... the entire spectrum of clinical research, is proud to ... the company,s comprehensive SaaS-based eClinical technology platform, has led ... the first two quarters of 2015.   Q2 2015 ... largest quarters measured by contract value sold in the ...
Breaking Biology News(10 mins):Sarcos to Debut First-of-its-Kind Snake Robot 2Sarcos to Debut First-of-its-Kind Snake Robot 3NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 2NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 3NXT-ID, Inc. Joins Smart Card Alliance and EMV Migration Forum 4MedNet Solutions Experiences Explosive Corporate Growth 2
... United States and Great Britain will speak at The ... and his theory of evolution, which continues to excite ... Darwins death. , The free one-day symposium will be ... 5:00 p.m. at The Field Museums James Simpson Theater. ...
... Darwin Foundation (CDF) botanists have published a list of ... Island, the third largest town in Galapagos. 261 species ... Despite 95% of the archipelago falling under the ... areas are having large impacts on the native flora ...
... in an intermittent on/off switch for a gene involved in ... found in a study of human brain tissue. The ... high rates during normal development of the prefrontal cortex, the ... and decision-making but that this normal increase may not ...
Cached Biology News:Darwin Symposium at Field Museum offers broad overview of his science and its impact 2Darwin Symposium at Field Museum offers broad overview of his science and its impact 3How schizophrenia develops: Major clues discovered 2
...
... White walled plates enhance luminescent signals ... fluorescence,• Not treated (or medium binding) ... and binds biomolecules through passive interactions,• ... of large molecules, such as antibodies, ...
... H-IPMIK-OH Lyophilized solid. Supplied as ... PACKAGED UNDER INERT GAS. A cell-permeable mutated ... No. 196810) that serves as a negative ... μM). Purity: ≥97% by HPLC. ...
...
Biology Products: