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Genomic DNA Isolation Protocol for 40 l Avian Whole Blood

tube containing 6 ml 100% isopropanol (2propanol).

2. Mix the sample by gently inverting the capped tube 50 times.

3. Centrifuge at 2,000 x g for 3 min; the DNA will be visible as a small white pellet.

4. Pour off the supernatant and drain tube on clean absorbent paper. Add 6 ml 70% ethanol and invert the capped tube several times to wash the DNA pellet.

5. Centrifuge at 2,000 x g for 1 min. Carefully pour off the ethanol. Pellet may be loose, so pour slowly and watch to ensure that pellet stays in the tube.

6. Invert and drain the tube on clean absorbent paper and allow to air-dry 15 min.

DNA Hydration
1. Add 500 l DNA hydration solution (500 l will give a concentration of 400 g/ml if the total yield is 200 g DNA).

2. Allow DNA to rehydrate overnight at room temperature. Alternatively, heat at 65C for 1 hr. Tap tube periodically to aid in dispersing the DNA.

3. Store DNA at 28C.


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