Navigation Links
Genomic DNA Isolation Protocol for 40 l Avian Whole Blood

tube containing 6 ml 100% isopropanol (2propanol).

2. Mix the sample by gently inverting the capped tube 50 times.

3. Centrifuge at 2,000 x g for 3 min; the DNA will be visible as a small white pellet.

4. Pour off the supernatant and drain tube on clean absorbent paper. Add 6 ml 70% ethanol and invert the capped tube several times to wash the DNA pellet.

5. Centrifuge at 2,000 x g for 1 min. Carefully pour off the ethanol. Pellet may be loose, so pour slowly and watch to ensure that pellet stays in the tube.

6. Invert and drain the tube on clean absorbent paper and allow to air-dry 15 min.

DNA Hydration
1. Add 500 l DNA hydration solution (500 l will give a concentration of 400 g/ml if the total yield is 200 g DNA).

2. Allow DNA to rehydrate overnight at room temperature. Alternatively, heat at 65C for 1 hr. Tap tube periodically to aid in dispersing the DNA.

3. Store DNA at 28C.


back to top
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. New Protocols for Isolating High- Molecular-Weight Genomic DNA
3. Easily Amplify Genomic DNA with Long-Distance PCR
4. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
5. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
6. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
7. Mouse Tail Genomic DNA Isolation Protocol(1)
8. Genomic DNA Isolation Protocol(1,2,3)
9. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
10. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
11. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Genomic DNA Isolation Protocol for Avian Whole Blood

(Date:7/25/2014)... Lawrence Livermore scientists Charles Westbrook and William ... " The World's Most Influential Scientific Minds ." , ... who were identified by analyzing citation data over the ... highest-impact work (2002-2012 and 2012-2013). The two were selected ... . , "This recognition is a great honor for ...
(Date:7/24/2014)... - July 24, 2014 -- Keryx Biopharmaceuticals, Inc. (Nasdaq:KERX) ... long-term, randomized, active control Phase 3 study of Zerenex ... binder, for the treatment of hyperphosphatemia in patients with ... (PhosphatE binding and iRon delivery with FErric CiTrate in ... of the American Society of Nephrology ( JASN ...
(Date:7/24/2014)... Calif. , July 24, 2014  ECC West ... Denver, Colorado with University College Hospital (UCH) ... a development project to finance, design, construct, and operate ... Nigeria . Under the first of ... prepare scoping documents and funding applications. UCH Ibadan intends ...
(Date:7/24/2014)... and synthetic processes prefer to settle into equilibriuma ... is within the realm of non-equilibrium conditions where ... in energy and phases, such as temperature fluctuations, ... humans to regulate their body temperature, airplanes to ... activity. , But even though these conditions ...
Breaking Biology Technology:Scientists Burst onto Influential List for Combustion Research 2Scientists Burst onto Influential List for Combustion Research 3Scientists Burst onto Influential List for Combustion Research 4Zerenex (ferric citrate) long-term Phase 3 study results published in JASN 2Zerenex (ferric citrate) long-term Phase 3 study results published in JASN 3Zerenex (ferric citrate) long-term Phase 3 study results published in JASN 4ECC Signs a Contract for the Finance, Design, and Construction of a Premier Cancer Institute in Ibadan, Nigeria 2New approach to form non-equilibrium structures 2
... SAN DIEGO, Oct. 4 Orexigen® Therapeutics, Inc. (Nasdaq: ... the treatment of obesity, today announced the schedule of ... taking place at the 28th Annual Scientific Meeting of ... the San Diego Convention Center, October 8-12, 2010.Sunday, October ...
... PDL BioPharma, Inc. (PDL) (Nasdaq: PDLI ) ... special dividend payment of $0.50 per share to all stockholders ... record date. As previously announced, the conversion ... 15, 2012 was adjusted to 140.571 shares of common stock ...
... 1 Biotech posted its strongest monthly and quarterly performance ... September rally for equities in more than 70 years. The ... and 13 percent in the third quarter. The Dow Jones ... appreciation during what historically has been a bad month for ...
Cached Biology Technology:Orexigen® Therapeutics Announces Upcoming Data Presentations at the 28th Annual Scientific Meeting of The Obesity Society 2Biotech Posts Strong Third Quarter Performance as Capital Markets Go on a Tear 2Biotech Posts Strong Third Quarter Performance as Capital Markets Go on a Tear 3Biotech Posts Strong Third Quarter Performance as Capital Markets Go on a Tear 4
(Date:7/25/2014)... a molecular testing panel developed at UPMC greatly ... surgery for patients with thyroid nodules and cancer, ... Institute (UPCI), partner with UPMC CancerCenter., The test, ... other diagnostic testing agencies, improved the chances of ... percent, according to the study published this month ...
(Date:7/24/2014)... NEW YORK, NY (July 24, 2014) Scientists ... Research Institute are one step closer to creating ... from a patient,s own cells. , For ... stem (iPS) cells lines from skin samples of ... they developed an accelerated protocol to induce these ...
(Date:7/24/2014)... /PRNewswire-iReach/ -- The Geneva Healthcare Suite, an innovative technology ... emergency room wait times by an average of 92 ... according to a recent study published in the Academic ... the suite to access data from incoming patients, implanted ... "Using Geneva,s technology platform we have been able to ...
Breaking Biology News(10 mins):Test increases odds of correct surgery for thyroid cancer patients 2Test increases odds of correct surgery for thyroid cancer patients 3NYSCF scientists one step closer to cell therapy for multiple sclerosis patients 2UC San Diego Medical Center Cuts Average ER Wait Time by 92 Minutes using the Geneva Healthcare Suite 2UC San Diego Medical Center Cuts Average ER Wait Time by 92 Minutes using the Geneva Healthcare Suite 3
... to develop and evaluate new tools to control the ... five year, 12 million project involving sixteen partners in ... Framework programme. Because malaria is transmitted by mosquitoes, ... Wide scale use of insecticides on bednets and ...
... has long been used as a crop fertilizer, but the ... in manure is ever present. The ratio of nitrogen to ... needs of most crops. Therefore, crops tend to be overloaded ... but the excess phosphorus from the process can damage the ...
... "The rapid translation of biologically-based laboratory discoveries into daily ... trip back from the clinic to the laboratory, to ... behavior. It is imperative for basic researchers to understand ... the knowledge and skills to integrate and use new ...
Cached Biology News:€12 million ($16.9 million) project to develop new tools for malaria control 2
UltraPure DNase/RNase-Free Distilled Water is designed for use in all molecular biology applications. It is 0.1-m membrane-filtered and tested for DNase and RNase activity....
...
... suitable for use with RNA. ... with 0.1% diethylpyrocarbonate (DEPC) and ... DEPC.DNase/RNase-Free Distilled WaterDistilled Water is ... molecular biology applications. It is ...
IHC detection kit for BrdU in cells and tissues...
Biology Products: