Navigation Links
Genomic DNA Isolation Protocol for 40 l Avian Whole Blood


AquaPure Genomic DNA Isolation Kit
Catalog #732-6340
Expected yield range: 100300 g DNA


Method
Cell Lysis
1. Use fresh blood within 10 sec of venipuncture to avoid clotting. Alternatively, collect whole blood in EDTA to prevent the blood from clotting and reduce DNA degradation. Blood may be either fresh or frozen. Store fresh blood sample at 28C for not more than 5 days to obtain optimal DNA isolation results.

2. Add 40 l whole blood to a 15 ml microfuge tube containing 6 ml cell lysis solution. Quickly pipet up and down 35 times to lyse the cells. Usually no incubation is required; however, if cell clumps are visible after mixing, incubate at 37C until the solution is homogeneous. Samples are stable in cell lysis solution for at least 18 months at room temperature.

RNase Treatment (Optional)
1. Add 30 l RNase A solution to the cell lysate.

2. Mix the sample by capping the tube and inverting it 25 times, then incubate at 37C for 1560 min.

Protein Precipitation
1. Cool sample to room temperature.

2. Add 2 ml protein precipitation polution to the RNase A treated cell lysate.

3. Vortex vigorously at high speed for 20 sec to mix the protein precipitation solution uniformly with the cell lysate.

4. Centrifuge at 2,000 x g for 10 min. The precipitated proteins will form a tight pellet. If the protein pellet is not visible or is not tight, repeat step 3, incubate on ice for 5 min, then repeat step 4.

DNA Precipitation
1. Leaving behind the precipitated protein pellet, pour the supernatant containing the DNA into a 15 ml centrifuge
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. New Protocols for Isolating High- Molecular-Weight Genomic DNA
3. Easily Amplify Genomic DNA with Long-Distance PCR
4. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
5. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
6. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
7. Mouse Tail Genomic DNA Isolation Protocol(1)
8. Genomic DNA Isolation Protocol(1,2,3)
9. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
10. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
11. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Genomic DNA Isolation Protocol for Avian Whole Blood

(Date:10/20/2014)... , Oct. 20, 2014 Asterias ... the Company has signed a Notice of Grant ... Medicine (CIRM), effective October 1, 2014.  The NGA ... payments and the release of additional grant funds ... grant award for clinical development of Asterias, product, ...
(Date:10/20/2014)... 20, 2014 PureTech , a science ... healthcare problems, announced today the closing of a ... from Invesco Perpetual, a $120 Billion group of ... PureTech,s existing pipeline forward and to advance new ... scientific creativity to really go for the big ...
(Date:10/19/2014)... The Latin American hardware encryption display market report ... analysis and forecast of revenue. This market was valued ... reach $2,366.8 million by 2018, at a CAGR of ... TOC of the Latin American hardware encryption market report ... It also provides a glimpse of the segmentation of ...
(Date:10/19/2014)... (PRWEB) October 19, 2014 The Asian ... in Asian with analysis and forecast of revenue. The ... grow to around $463.9 million by 2018, at a ... Browse through the TOC of the Asian Automatic patient ... analysis provided. This also provides a glimpse of the ...
Breaking Biology Technology:Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 2Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 3Asterias Biotherapeutics Announces Notice of Grant Award with CIRM for Phase 1/2a Clinical Trial of AST-OPC1 in Complete Cervical Spinal Cord Injury 4PureTech Announces Expansion with Oversubscribed $55M Funding Round 2PureTech Announces Expansion with Oversubscribed $55M Funding Round 3PureTech Announces Expansion with Oversubscribed $55M Funding Round 4PureTech Announces Expansion with Oversubscribed $55M Funding Round 5PureTech Announces Expansion with Oversubscribed $55M Funding Round 6The Latin American hardware encryption display market is expected to reach $2,366.8 million by 2018 - New Report by MicroMarket Monitor 2The Latin American hardware encryption display market is expected to reach $2,366.8 million by 2018 - New Report by MicroMarket Monitor 3The Asian Automatic patient billing market is estimated to grow to around $463.9 million by 2018 - New Report by MicroMarket Monitor 2The Asian Automatic patient billing market is estimated to grow to around $463.9 million by 2018 - New Report by MicroMarket Monitor 3
... - MacoPharma increased its forecast for P-Capt(R) filters sale in 2008;, - Use of affinity prion adsorbent for ... in excess of $25 M over the next 5 years ... - $4 M in 2008;,- 10th product approved by the FDA and or the EMEA, contributing ... growth., MONTREAL, ...
... Cholesterol Test Can Help Identify Patients At Risk of Heart ... ... Atherotech, Inc., a,cardio-diagnostic company, today announced that the largest private,insurers in ... (FEHB) Program now cover the VAP (Vertical Auto Profile),Test, the most ...
... Software Platform Further Solidifies Netsmart as Leader in Behavioral Healthcare ... ... Florida, GREAT RIVER, N.Y., Feb. 6 Netsmart Technologies, Inc.,a ... organizations, today announced it has signed a contract to,provide comprehensive licensed ...
Cached Biology Technology:ProMetic provides business update - Over $35 million worth of business secured in January 2ProMetic provides business update - Over $35 million worth of business secured in January 3ProMetic provides business update - Over $35 million worth of business secured in January 4VAP Cholesterol Test From Atherotech Covers 90 Percent of Arizona Insured 2Netsmart Technologies Extends Relationship with Manatee Glens for Enterprise Behavioral Health Software Solutions 2Netsmart Technologies Extends Relationship with Manatee Glens for Enterprise Behavioral Health Software Solutions 3
(Date:10/18/2014)... 2,000 patients referred for evaluation of suspected genetic ... for 25 percent, including detection of a number ... to disease, according to a study appearing in ... to coincide with the American Society of Human ... exons or coding regions of thousands of genes ...
(Date:10/17/2014)... German . Why ... order to reproduce? And why are there two sexes anyway? ... issue of the research journal Molecular Human Reproduction ... from Bielefeld University Bielefeld has compiled this special issue on ... female to copulate with several males in quick succession – ...
(Date:10/16/2014)... Cancer constantly wages war on the human body. ... a stalemate. In pancreatic cancer, this stalemate—known as ... becoming aggressively malignant, a phenomena that is poorly ... in the laboratory of Salvatore Torquato, a Professor ... the conditions surrounding tumor dormancy and the switch ...
Breaking Biology News(10 mins):Whole-exome sequencing shows potential as diagnostic tool 2Sperm wars 2Modeling tumor dormancy 2
... Food and Drug Administration (FDA) has granted approval to ... Alza Corporation's Duragesic Patch (Fentanyl Transdermal System) used to ... be managed with alternative analgesics. When applied to the ... medication that is slowly absorbed into the body through ...
... found that introducing a growth factor protein into a mouth ... according to a new paper in the February issue of ... sizeable mouth wound, replacing a tooth takes more than simply ... to anchor the new tooth in place. Such reconstructive surgery ...
... protein called IRE1, which helps stressed-out cells make new ... believed. A new study by researchers from the University ... Institute reveals the surprising finding that this same signaling ... called B lymphocytes. , "This is a unique signaling ...
Cached Biology News:Gene therapy promising for growing tooth-supporting bone 2Gene therapy promising for growing tooth-supporting bone 3Multi-purpose protein regulates new protein synthesis and immune cell development 2Multi-purpose protein regulates new protein synthesis and immune cell development 3
Molecular Biology grade. Ideally suited for DNA and RNA separation and recovery. High gel strength. Gelling temp. 36C. Melting temp. 88C. EEO: 0.05-0.13 Resolution 0.2-40 kb; Rnase/Dnase: none detec...
... offers the most comprehensive, cost-effective solution ... have incorporated unprecedented quality into both ... includes proven detection chemistries and antibodies ... TechMate protocol. It also includes our ...
Human Cell Line Slides...
Mouse Cell Line Slides...
Biology Products: