Navigation Links
Genomic DNA Isolation Protocol for 40 l Avian Whole Blood


AquaPure Genomic DNA Isolation Kit
Catalog #732-6340
Expected yield range: 100300 g DNA


Method
Cell Lysis
1. Use fresh blood within 10 sec of venipuncture to avoid clotting. Alternatively, collect whole blood in EDTA to prevent the blood from clotting and reduce DNA degradation. Blood may be either fresh or frozen. Store fresh blood sample at 28C for not more than 5 days to obtain optimal DNA isolation results.

2. Add 40 l whole blood to a 15 ml microfuge tube containing 6 ml cell lysis solution. Quickly pipet up and down 35 times to lyse the cells. Usually no incubation is required; however, if cell clumps are visible after mixing, incubate at 37C until the solution is homogeneous. Samples are stable in cell lysis solution for at least 18 months at room temperature.

RNase Treatment (Optional)
1. Add 30 l RNase A solution to the cell lysate.

2. Mix the sample by capping the tube and inverting it 25 times, then incubate at 37C for 1560 min.

Protein Precipitation
1. Cool sample to room temperature.

2. Add 2 ml protein precipitation polution to the RNase A treated cell lysate.

3. Vortex vigorously at high speed for 20 sec to mix the protein precipitation solution uniformly with the cell lysate.

4. Centrifuge at 2,000 x g for 10 min. The precipitated proteins will form a tight pellet. If the protein pellet is not visible or is not tight, repeat step 3, incubate on ice for 5 min, then repeat step 4.

DNA Precipitation
1. Leaving behind the precipitated protein pellet, pour the supernatant containing the DNA into a 15 ml centrifuge
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. New Protocols for Isolating High- Molecular-Weight Genomic DNA
3. Easily Amplify Genomic DNA with Long-Distance PCR
4. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
5. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
6. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
7. Mouse Tail Genomic DNA Isolation Protocol(1)
8. Genomic DNA Isolation Protocol(1,2,3)
9. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
10. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
11. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Genomic DNA Isolation Protocol for Avian Whole Blood

(Date:7/10/2014)... 10, 2014 OMICS Group’s 5th International ... 18-20 August, 2014 at Double Tree by Hilton Beijing, ... developments in Analytical & Bioanalytical research methods only to ... on this occasion, Dr. Srinubabu Gedela, MD of OMICS ... is a remarkable one in bringing a unique and ...
(Date:7/10/2014)... 2014  Kainos Capital, a firm specializing in acquiring ... it has acquired the Slim-Fast brand from Unilever. Unilever ... of the transaction were not disclosed. Slim-Fast ... that markets ready-to-drink shakes, powders, bars and snacks to ... and in the United Kingdom ...
(Date:7/10/2014)... in the fight against terrorism with the creation of ... with the use of light and special glass fibres. ... B: Chemical , the researchers describe a novel optical ... low as 6.3 ppm (parts per million). It requires ... "Traditionally explosives detection has involved looking for metals that ...
(Date:7/10/2014)... Wash. The lithium-ion batteries that power our laptops ... longer on a single charge with the help of ... porous material to replace the graphite traditionally used in ... than 10 times the energy storage capacity of graphite. ... battery electrode was published today in Nature Communications ...
Breaking Biology Technology:A Critical Review of Analytical Techniques for Improved Living Standards 2A Critical Review of Analytical Techniques for Improved Living Standards 3A Critical Review of Analytical Techniques for Improved Living Standards 4Kainos Capital Acquires Slim-Fast From Unilever 2Detecting trace amounts of explosives with light 2Silicon sponge improves lithium-ion battery performance 2Silicon sponge improves lithium-ion battery performance 3
... The ... available leg-room, energy efficient impellers, and new service-friendly control panel, the Allergard is an ... ... Animal Transfer Station (ATS) from NuAire is ergonomic, portable, and energy efficient. Lightweight design ...
... ... easier to learn about the oldest buildings on earth and a “fascinatingly weird” ancient culture. ... (PRWEB) June 11, ... on its website to provide a simple introduction to megalithic architects in Frequently Asked Question ...
... ... in clinical research, key issues around open source technology are highlighted at leading industry events. ... Washington, DC (PRWEB) June ... meeting of the Drug Information Association (DIA). The DIA Annual Meeting is the ...
Cached Biology Technology:The Allergard 619: an All-new Animal Transfer Station from NuAire 2New tool for exploring the ancient world. 2Open Source Clinical Trials Software Featured at the 46th DIA Annual Meeting 2Open Source Clinical Trials Software Featured at the 46th DIA Annual Meeting 3
(Date:7/11/2014)... were many fires burning in eastern New South Wales, ... 11. , At 03:35 UTC on July 11 (12:35 ... Aqua passed over eastern New South Wales (NSW), the ... visible image of the region and spotted smoke (light ... MODIS,s thermal bands, are outlined in red. , The ...
(Date:7/11/2014)... Cell scientists have set a "mouse TRAP" to capture ... a recent study published in the Journal of ... a technique called TRAP to extract cellular and genetic ... by scientists at the Rockefeller Institute for Medical Research ... the protein-making machinery, or ribosomes, of the cell type ...
(Date:7/11/2014)... from the Max Planck Institute for Medical Research in ... understanding photosynthesis, the process by which the Earth first ... and which is therefore crucial for all higher forms ... the first direct visualization of a crucial event in ... specific protein complex, photosystem II, splits water into hydrogen ...
Breaking Biology News(10 mins):A first direct glimpse of photosynthesis in action 2
... a pickle People taking care of more ... parents -- engaged in fewer healthy behaviors, found a ... University. As the U.S. population continues to age, more ... the research team to conclude that "encouraging healthy behaviors ...
... Researchers from the John Innes Centre in Norwich, Great ... the FLORA project, have obtained genetically modified tomatoes rich ... class of flavonoids. These tomatoes, added to the diet ... extending the mice lifespan. The research has been published ...
... snapdragon in tomatoes to grow purple tomatoes high ... pigments found at particularly high levels in berries ... investigating ways to increase the levels of health-promoting ... "Most people do not eat 5 portions ...
Cached Biology News:Indiana University research at American Public Health Association meeting 2Indiana University research at American Public Health Association meeting 3Indiana University research at American Public Health Association meeting 4Indiana University research at American Public Health Association meeting 5Purple tomatoes: The richness of antioxidants against tumors 2Purple tomatoes: The richness of antioxidants against tumors 3Fried purple tomatoes 2
...
DNA fragmentation factor & Inhibitor of CAD...
Component in MasterPure™ Purification Kits...
Anti-gp340 Monoclonal Antibody Description: 100 g Research Focus: cancer & cell proliferation Storage: -20C Shipping Temperature: 4C...
Biology Products: