Navigation Links
Genomic DNA Isolation Protocol(1,2,3)

tube containing the deproteinized DNA. Cap the tube tightly.
  • Mix by shaking vigorously enough to form a homogeneous suspension. Do not vortex.
  • Centrifuge at 1500 x g for 5 minutes to isolate the upper, DNA-containing aqueous phase away from the lower, organic solvent phase.
  • Carefully decant upper phase containing the DNA into a fresh, pre-spun, PLG 15 ml Light tube.
  • Repeat steps 1 through 3 but this time extracting with 4 ml water-saturated Phenol-Chloroform (PC, 1:1).
  • Carefully decant upper phase containing the DNA into a clean 15 ml polypropylene centrifuge tube.

    Proceed to Section IV.

  • IV Precipitation of DNA

    At this point, the DNA should have been extracted with both Phenol and Phenol-Chloroform and should be in a clean 15 ml polypropylene screw cap centrifuge tube.

    1. Add 100 l 2 M KCI and mix by gentle inversion.
    2. Overlay DNA solution with 5 ml 95% Ethanol by slowly pipetting the Ethanol down the side of the tube.
    3. Place a Pasteur pipet tip at the interface of the DNA-Ethanol solution and spool the DNA onto the pipet tip by swirling the pipet, keeping the tip at the interface, until the 2 phases are completely mixed.
    4. Place pipet tip with the spooled DNA in 1 ml 70% Ethanol for about 2 minutes.
    5. Remove pipet from the 70% Ethanol and hold upright (tip up) for a few seconds to allow the excess Ethanol to drain away. Do not allow the DNA to dry.
    6. Set pipet tip in a microcentrifuge tube containing 200 l TE (10 mM Tris-Cl, 1 mM EDTA, pH 8.0) and incubate f
      '"/>


    Source:


    Page: All 1 2 3 4

    Related biology technology :

    1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
    2. New Protocols for Isolating High- Molecular-Weight Genomic DNA
    3. Easily Amplify Genomic DNA with Long-Distance PCR
    4. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
    5. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
    6. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
    7. Mouse Tail Genomic DNA Isolation Protocol(1)
    8. Genomic DNA Isolation Protocol(1,2,3)
    9. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
    10. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
    11. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
    Post Your Comments:
    *Name:
    *Comment:
    *Email:
    TAG: Genomic DNA Isolation Protocol

    (Date:11/24/2014)... November 24, 2014 2014 Deep ... Industry is a professional and in-depth research report ... Dihydrazide information, like its definition, classification, application, and ... This report covers the international market analysis, including ... as the US, Europe, Asia, China, Japan etc. ...
    (Date:11/24/2014)... Md. , Nov. 24, 2014  Spherix ... development company committed to the fostering and monetization ... Spherix v. VTech Telecommunications Ltd.,  Case No. 3:13-cv-03494-M ... Case No. 3:13-cv-03496-M, both in the United States ... Texas . On ...
    (Date:11/23/2014)... Francis, WI (PRWEB) November 23, 2014 ... manufacturer and supplier of fine chemical ingredients to ... announced the launch of its new website, Apiscent.com. ... description of Apiscent Labs, clean design, easy navigation, ... that make Apiscent Labs stand out. ...
    (Date:11/22/2014)... (PRWEB) November 21, 2014 During his ... understood the need to surround himself with great people ... and entrepreneur, his friends often marveled at his extraordinarily ... lived his life and -- even with his death ... the Alzheimer’s disease that would ultimately take his life. ...
    Breaking Biology Technology:Adipic Dihydrazide Industry 2019 Research Forecasts on Worldwide, China Regions Now Available at ReportsnReports.com 2Adipic Dihydrazide Industry 2019 Research Forecasts on Worldwide, China Regions Now Available at ReportsnReports.com 3Markman Hearing Held as Scheduled in VTech and Uniden Cases 2Sherry Sharp, Wife of CarMax Founder, Richard Sharp, Accepts Seat on Board of Cure Alzheimer’s Fund 2Sherry Sharp, Wife of CarMax Founder, Richard Sharp, Accepts Seat on Board of Cure Alzheimer’s Fund 3
    ... May 18 ViroPharma Incorporated (Nasdaq: VPHM ... National Organization for Rare Disorders (NORD) Annual Gala for ... to market for a rare and devastating disorder -- ... genetic disorder caused by a deficiency of C1 inhibitor, ...
    ... Debt Facility; Support Profitable GrowthALISO VIEJO, Calif., May 18 ... CLRT ), a premier anatomic pathology and molecular ... industry, announced today that it has completed the second ... with Oak Investment Partners , a ...
    ... and Merieux Alliance,have signed a letter of intent for ... The Institut Pasteur and Merieux Alliance (which comprises the ... to,pool their resources and efforts to fight infectious diseases ... - Clinical diagnostics and theranostics (in ...
    Cached Biology Technology:ViroPharma Incorporated Honored by the National Organization for Rare Disorders 2ViroPharma Incorporated Honored by the National Organization for Rare Disorders 3ViroPharma Incorporated Honored by the National Organization for Rare Disorders 4ViroPharma Incorporated Honored by the National Organization for Rare Disorders 5Clarient Completes Second Tranche of Private Placement of Convertible Preferred Stock With Oak Investment Partners 2Clarient Completes Second Tranche of Private Placement of Convertible Preferred Stock With Oak Investment Partners 3Clarient Completes Second Tranche of Private Placement of Convertible Preferred Stock With Oak Investment Partners 4Clarient Completes Second Tranche of Private Placement of Convertible Preferred Stock With Oak Investment Partners 5Clarient Completes Second Tranche of Private Placement of Convertible Preferred Stock With Oak Investment Partners 6The Institut Pasteur and Merieux Alliance Take First Step Towards a Long-Term Research Partnership in the Field of Infectious Diseases 2The Institut Pasteur and Merieux Alliance Take First Step Towards a Long-Term Research Partnership in the Field of Infectious Diseases 3
    (Date:11/5/2014)... diversity in their ability to identify scents and odors. ... their perceptual evaluation of odors, with women outperforming men ... Sex differences in olfactory detection may play a role ... one,s perception of smell, which is naturally linked to ... been suggested to be cognitive or emotional, rather than ...
    (Date:11/4/2014)... the right amount of death at the right time ... to new research that could help in understanding animal ... , In a paper in the journal Trends ... and European colleagues conclude that the kind of positive ... of individuals, or mortality, depends on the size and ...
    (Date:11/4/2014)... GABLES, Fla. (November 4, 2014) — Think about the ... ask: How do neighboring cells know that they are ... and how do these tissues find the correct place ... are answering these crucial questions. , In a new ... use to communicate with their surrounding neighbors, at the ...
    Breaking Biology News(10 mins):The female nose always knows: Do women have more olfactory neurons? 2When less is more: Death in moderation boosts population density in nature 2The inside story: How the brain and skull stay together 2
    ... Larry Murdoch have received an $891,000 Environmental Protection Agency ... in geological formations located deep below the earth,s surface. ... believed to be the leading cause of global warming, ... most promising alternatives for reducing emissions using current technology. ...
    ... . The DFG has approved the establishment of ... decision was made recently by the relevant Grants Committee in Bonn. ... a total of 132 million. In addition, 20 percent overhead funding ... centres, research topics range from modern prostheses to political reforms to ...
    ... Journal of Experimental Biology are pleased to announce ... Scientifique (CNRS) in the Universit de Nice-Sophia-Antipolis is the winner ... prize is awarded in recognition of an outstanding achievement and ... JEB Editor-in-Chief, and adds that ,we truly appreciate ...
    Cached Biology News:DFG approves funding for 17 new collaborative research centers 2DFG approves funding for 17 new collaborative research centers 3DFG approves funding for 17 new collaborative research centers 4DFG approves funding for 17 new collaborative research centers 5DFG approves funding for 17 new collaborative research centers 6DFG approves funding for 17 new collaborative research centers 7DFG approves funding for 17 new collaborative research centers 8DFG approves funding for 17 new collaborative research centers 9French scientist wins the Journal of Experimental Biology Outstanding Paper Prize 2009 2French scientist wins the Journal of Experimental Biology Outstanding Paper Prize 2009 3French scientist wins the Journal of Experimental Biology Outstanding Paper Prize 2009 4
    ... serum is collected from fasted Rhesus monkeys of ... Available Anticoagulants: N-02: Citrate ... K2EDTA N-08: Potassium Oxalate N-10: ... N-05: ACD N-07: CPD ...
    Porcine Serum US Origin 6 Months or Older...
    SEQUENASE PCR PRODUCT PRE-SEQU, 1 EA. Category: SEQUENASE....
    ... is collected off the clot from fasted Cynomolgus monkeys ... Available Anticoagulants: N-02: Citrate ... N-08: Potassium Oxalate N-10: EDTA (Na) ... N-07: CPD N-09: K3EDTA N-11: ...
    Biology Products: