Navigation Links
Genomic DNA Isolation Protocol(1,2,3)

ge tube.
  • Pellet cells by centrifugation at 250 x g for 5 minutes at 4C.
  • Decant supernatant and drain any residual supernatant by inverting the tube on a paper towel for 2 minutes.
  • Suspend cells in 1 ml 1x TBS by repeated up and down pipetting, then add 4 ml 1x TBS and 5 ml 2x Lysis Buffer to the cell suspension and mix by inversion.
  • After a 5 minute incubation on ice, pellet nuclei by centrifugation at 1100 x g for 12 minutes at 4C.
  • Decant supernatant and drain any residual supernatant by inverting the tube on a paper towel for 2 minutes.
    Note: The nuclei may be stored at 70C for several weeks at this point, however, higher molecular weight DNA is obtained from freshly prepared nuclei.

    Proceed to Section II.

  • II Lysis of nuclei and deproteinization of DNA
    1. Suspend pelleted nuclei in 2 ml Saline/EDTA Solution (75 mM NaCl, 24 mM EDTA) by repeated up and down pipetting.
    2. Carefully transfer suspended nuclei to a pre-spun (1500 x g for 23 minutes) PLG 15 ml Light tube.
    3. Add 50 L 20 mg/ml Proteinase K (in 10 mM Tris-Cl, pH 8.0, 1.0 mM CaCl2, 30% Glycerol) and 200 l 10% SDS to the suspended nuclei and mix by gentle inversion.
    4. Incubate for 2 hours at 37C with occasional gentle mixing. Note: This incubation may be extended to 1618 hours if that is more convenient.

      Proceed to Section III.

    III Extraction of protein
    1. Add 4 ml water-saturated Phenol to the PLG 15 ml Light
      '"/>


    Source:


    Page: All 1 2 3 4

    Related biology technology :

    1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
    2. New Protocols for Isolating High- Molecular-Weight Genomic DNA
    3. Easily Amplify Genomic DNA with Long-Distance PCR
    4. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
    5. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
    6. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
    7. Mouse Tail Genomic DNA Isolation Protocol(1)
    8. Genomic DNA Isolation Protocol(1,2,3)
    9. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
    10. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
    11. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
    Post Your Comments:
    *Name:
    *Comment:
    *Email:
    TAG: Genomic DNA Isolation Protocol

    (Date:2/27/2015)... 27, 2015   PureTech , a science and ... disruptive products and technologies in the healthcare sector, announced ... CEO and Member of the Board of Directors of ... "It,s has been a pleasure to know Chris for ... will be working together more closely now," said Dr. ...
    (Date:2/26/2015)... TORONTO , Feb. 26, 2015 /PRNewswire/ - SQI ... SQIDF), a life sciences company that develops and commercializes ... reported its financial and operational results for the fiscal ... this press release are in Canadian dollars (CAD), unless ... Company continued to win new business from our existing ...
    (Date:2/26/2015)... Feb. 26, 2015 DuPont Executive Vice President ... by key research advancements and product launches across the ... of America Merrill Lynch 2015 Global Agriculture Conference. ... building grain supplies the last two years, long-term demand ... pace of the last decade, when demand for corn ...
    (Date:2/26/2015)... , Feb. 26, 2015 The healthcare landscape ... ever-changing arena, Frost & Sullivan will host its 20 ... Executive MindXchange , March 8 to 10, at the Hilton ... Calif. This event is a platform ... leaders to discuss industry disruptions and how to succeed in ...
    Breaking Biology Technology:Christopher Viehbacher, former CEO of Sanofi, Joins PureTech's Board of Directors 2Christopher Viehbacher, former CEO of Sanofi, Joins PureTech's Board of Directors 3Christopher Viehbacher, former CEO of Sanofi, Joins PureTech's Board of Directors 4SQI Diagnostics Reports First Quarter Financial Results 2SQI Diagnostics Reports First Quarter Financial Results 3SQI Diagnostics Reports First Quarter Financial Results 4SQI Diagnostics Reports First Quarter Financial Results 5SQI Diagnostics Reports First Quarter Financial Results 6DuPont Leader Outlines Path for Growth in Agriculture and Nutrition & Health Segments 2DuPont Leader Outlines Path for Growth in Agriculture and Nutrition & Health Segments 3DuPont Leader Outlines Path for Growth in Agriculture and Nutrition & Health Segments 4DuPont Leader Outlines Path for Growth in Agriculture and Nutrition & Health Segments 5Embrace the Future of Medical Technologies with Frost & Sullivan 2Embrace the Future of Medical Technologies with Frost & Sullivan 3
    ... 17, 2009 SAN DIEGO, March 10 Neurocrine Biosciences, ... Kevin Gorman, President and Chief Executive Officer of Neurocrine ... 29th Annual Health Care Conference in Boston. The live ... a.m. Eastern Time (ET)/7:15 a.m. Pacific Time (PT). ...
    ... N.C., March 10 Campbell Alliance, the ... biotech industry, today announced that Ashwin Dandekar, Vice President, ... Oncology Summit USA on March 30 and 31, 2009 ... Dr. Dandekar will also present a workshop at ...
    ... Market Share Away from Lucentis from 2012 to ... ResourcesWALTHAM, Mass., March 10 Decision Resources, one ... for pharmaceutical and healthcare issues, finds that robust ... degeneration (AMD) market through 2012 will be driven ...
    Cached Biology Technology:Campbell Alliance Brand Management Practice to Present at EyeForPharma's Oncology Summit USA and Oncology Market Access Europe Conference 2Campbell Alliance Brand Management Practice to Present at EyeForPharma's Oncology Summit USA and Oncology Market Access Europe Conference 3Robust Annual Growth Through 2012 in the Age-Related Macular Degeneration Market Will Be Driven by Expanding Use of Lucentis in Europe 2Robust Annual Growth Through 2012 in the Age-Related Macular Degeneration Market Will Be Driven by Expanding Use of Lucentis in Europe 3
    (Date:2/24/2015)... DUBLIN , Feb. 24, 2015 Research ... announced the addition of the "Global 2D ... offering. The analysts forecast the ... a CAGR of 32.12% over the period 2014-2019 ... devices in non-traditional sectors is one of the ...
    (Date:2/19/2015)... DUBLIN , Feb .19, 2015 Research ... announced the addition of the "Military Electro-Optical ... by Sensor Technology, by Platform - Forecast to ... The military electro-optical/infrared systems market is expected ... a CAGR of 7.71%. This report ...
    (Date:2/13/2015)... , Feb. 13, 2015 ACT Genomics ... that aims to transform cancer genomic information into ... the company has raised US$ 8 million in the ... in Taipei, Taiwan , ACT ... ACTOnco™. With the aim to implement next generation ...
    Breaking Biology News(10 mins):Global 2D Gesture Recognition Market 2015-2019 with Cognitec Systems, CogniVue, eyeSight Mobile Technologies & PointGrab Dominating 2Military Electro-Optical / Infrared Systems (Image Intensification, Laser, Infrared) Market - Forecast to 2020: Featuring Profiles of the Market Leaders on this $16 Billion market 2Military Electro-Optical / Infrared Systems (Image Intensification, Laser, Infrared) Market - Forecast to 2020: Featuring Profiles of the Market Leaders on this $16 Billion market 3ACT Genomics Raises $8 Million in its First Private Funding Round 2
    ... in the retail nursery industry and with many home ... a variety of plants, conserve water, and reduce the ... lined with polyethylene film on the bottom and covered ... mats help minimize evaporation while allowing water to move ...
    ... uncharted tenants of the bloodstream known mostly for ... also rally sustained immune system inflammatory responses that ... according to a new report from Johns Hopkins ... aspects of transplant biology," says Craig Morrell, D.V.M., ...
    ... for Disease Control and Prevention (CDC) shows a ... methicillin-resistant and methicillin-sensitive S. aureus . The ... Central Line-Associated Bloodstream Infections in US Intensive Care ... Nosocomial Infection Surveillance (NNIS) and its successor, the ...
    Cached Biology News:Capillary mats labor-saving, economical alternative to hand watering 2Researchers explore new driver of transplant rejection: Platelets 2Researchers explore new driver of transplant rejection: Platelets 3Press statement on new CDC MRSA study from SHEA president 2
    Recombinant Rat CINC-2 alpha, CF...
    RAT ANTI MOUSE F4/80 ANTIGEN:BIOTIN Immunogen: Thioglycollate stimulated peritoneal macrophages from C57/BL mice...
    4-Hydroxy-3-nitrophenylacetyl hapten is conjugated to SGG (Sheep gamma globulin) lysine through amide bonds. This product is homologous to NP-BSA....
    RAT ANTI MOUSE F4/80 ANTIGEN:BIOTIN Immunogen: Thioglycollate stimulated peritoneal macrophages from C57/BL mice...
    Biology Products: