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Genomic DNA Isolation Protocol(1,2,3)

or 20 minutes at room temperature. The DNA should slide from the pipet into the TE. If necessary, gently remove the DNA from the pipet by scraping the DNA onto the interior of the tube.
  • Redissolve DNA completely by incubating overnight at 4C. Store DNA at 4C. Do not freeze.
  • References
    1. Herrmann, B.G. and Frischauf, A.M. (1987) Isolation of genomic DNA. Methods Enzymol. 152:180-183.
    2. Laws, G.M. and S.P. Adams. (1996) Measurement of 8-OHdG in DNA by HPLC/ECD: The importance of DNA purity. BioTechniques 20:36-38.
    3. Murphy, N.R. and Hellwig, R. J. (1996) Improved nucleic acid organic extraction through use of a unique gel barrier matrix. BioTechniques 21:934-939.

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