Navigation Links
Genomic DNA Isolation Protocol(1,2,3)

tube containing the deproteinized DNA. Cap the tube tightly.
  • Mix by shaking vigorously enough to form a homogeneous suspension. Do not vortex.
  • Centrifuge at 1500 x g for 5 minutes to isolate the upper, DNA-containing aqueous phase away from the lower, organic solvent phase.
  • Carefully decant upper phase containing the DNA into a fresh, pre-spun, PLG 15 ml Light tube.
  • Repeat steps 1 through 3 but this time extracting with 4 ml water-saturated Phenol-Chloroform (PC, 1:1).
  • Carefully decant upper phase containing the DNA into a clean 15 ml polypropylene centrifuge tube.

    Proceed to Section IV.

  • IV Precipitation of DNA

    At this point, the DNA should have been extracted with both Phenol and Phenol-Chloroform and should be in a clean 15 ml polypropylene screw cap centrifuge tube.

    1. Add 100 l 2 M KCI and mix by gentle inversion.
    2. Overlay DNA solution with 5 ml 95% Ethanol by slowly pipetting the Ethanol down the side of the tube.
    3. Place a Pasteur pipet tip at the interface of the DNA-Ethanol solution and spool the DNA onto the pipet tip by swirling the pipet, keeping the tip at the interface, until the 2 phases are completely mixed.
    4. Place pipet tip with the spooled DNA in 1 ml 70% Ethanol for about 2 minutes.
    5. Remove pipet from the 70% Ethanol and hold upright (tip up) for a few seconds to allow the excess Ethanol to drain away. Do not allow the DNA to dry.
    6. Set pipet tip in a microcentrifuge tube containing 200 l TE (10 mM Tris-Cl, 1 mM EDTA, pH 8.0) and incubate f
      '"/>


    Source:


    Page: All 1 2 3 4

    Related biology technology :

    1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
    2. New Protocols for Isolating High- Molecular-Weight Genomic DNA
    3. Easily Amplify Genomic DNA with Long-Distance PCR
    4. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
    5. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
    6. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
    7. Mouse Tail Genomic DNA Isolation Protocol(1)
    8. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
    9. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
    10. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
    11. Genomic DNA Isolation Protocol(1,2,3)
    Post Your Comments:
    *Name:
    *Comment:
    *Email:
    TAG: Genomic DNA Isolation Protocol

    (Date:9/19/2014)... that use light, rather than electricity, to move ... energy efficiency is a growing concern as chips, ... components of optical circuits light emitters, modulators, ... build. One promising light source for optical chips ... properties when deposited as a single, atom-thick layer. ...
    (Date:9/19/2014)... 2014  Nektar Therapeutics (NASDAQ: NKTR ... the analgesic profiles of a series of the ... molecules. The preclinical research candidates were created using ... The analgesic properties of kappa receptor agonism ... Kappa opioid receptors are expressed in the ...
    (Date:9/19/2014)... September 19, 2014 Cereal grain ... industries will gather October 5–8, 2014, for the ... Island Convention Center in Providence, Rhode Island. The ... work of food scientists, chemists, microbiologists, nutritionists, and ... food production management. To date, 1,000 attendees have ...
    (Date:9/18/2014)... Bipartisan passage this week by the U.S. ... development and commercialization of new technologies and promote growth ... SPIE, the international society for optics and photonics ... (RAMI) Act, now moves to the full Senate for ... optimistic that it will win passage there as well ...
    Breaking Biology Technology:Toward optical chips 2Toward optical chips 3Nektar Presents Positive Preclinical Data on Oral, Peripherally-Acting Kappa Agonist Molecules at 2014 American Academy of Pain Management Meeting 2Nektar Presents Positive Preclinical Data on Oral, Peripherally-Acting Kappa Agonist Molecules at 2014 American Academy of Pain Management Meeting 3Nektar Presents Positive Preclinical Data on Oral, Peripherally-Acting Kappa Agonist Molecules at 2014 American Academy of Pain Management Meeting 4Nektar Presents Positive Preclinical Data on Oral, Peripherally-Acting Kappa Agonist Molecules at 2014 American Academy of Pain Management Meeting 5Latest Research on Cereal Grain Quality, Health Benefits and Food Production to be Presented During the AACC International Annual Meeting 2Latest Research on Cereal Grain Quality, Health Benefits and Food Production to be Presented During the AACC International Annual Meeting 3House passage of high-tech manufacturing bill is move toward stronger economy, say SPIE leaders 2House passage of high-tech manufacturing bill is move toward stronger economy, say SPIE leaders 3
    ... AMLN ) will be presenting at the Morgan Stanley Global ... ET / 7:55 a.m. PT in ... Tuesday, September 14, 2010 at 11:30 a.m. ET / 8:30 ... president and chief executive officer of Amylin Pharmaceuticals, will provide a corporate ...
    ... has great potential to make electronic devices such as radios, ... have also led to difficulties in integrating the material into ... 1 in the journal Nature , a group of ... difficulties to fabricate the fastest graphene transistor to date. ...
    ... for Biochemistry and Molecular Biology expressed its disapproval and ... ruling in the U.S. District Court for the District ... funding of embryonic stem-cell research. In a ... response to a lawsuit filed by two adult stem-cell ...
    Cached Biology Technology:UCLA chemists, engineers achieve world record with high-speed graphene transistors 2American Society for Biochemistry and Molecular Biology Reacts to District Court Stem-Cell Ruling 2
    (Date:9/19/2014)... unveiled today by researchers from several Harvard University ... both experienced and aspiring researchers with the intellectual ... robots made from soft, flexible materials. , With ... and other advances in manufacturing technology, soft robotics ... principles drawn from conventional rigid robot design, but ...
    (Date:9/19/2014)... A University of Oklahoma biology professor will study ... control leg movements with a National Science Foundation ... four-year project. , Ari Berkowitz, professor in the ... Sciences, will focus on the differences between the ... understand what neurotransmitters they use and what connections ...
    (Date:9/19/2014)... a species which over thousands of years has adapted to ... substance that makes the cold dangerous for you. , This ... , and the cold provoking substance, called nonylphenol, comes from ... is suspected of being a endocrine disruptor, but when entering ... worm,s ability to protect the cells in its body from ...
    Breaking Biology News(10 mins):Soft robotics 'toolkit' features everything a robot-maker needs 2Environmental pollutants make worms susceptible to cold 2Environmental pollutants make worms susceptible to cold 3
    ... After several years of battling recurring infections, the last thing ... cause of her problems might actually help millions live longer, ... Goetzl and his colleagues from the University of California and ... unique antibody to her own T cells, the cells that ...
    ... Research has shown that certain marginalized groups including ... as African Americans and Latinos fare worse than ... impoverishment, injuries and fatalities. Now, ... found that they also experience greater terrorism-related fears and ...
    ... (January 21, 2008)- Jizhou Song, a professor in the ... collaborators Professor John Rogers, at the University of Illinois ... a new design for stretchable electronics that can be ... function. , The new mechanical design strategy is ...
    Cached Biology News:Unexpected finding opens up new way to stop autoimmune diseases and transplant rejection 2Disabled and other vulnerable groups more susceptible to terrorism fears 2University of Miami engineer designs stretchable electronics with a twist 2
    Human Legumain Affinity Purified Polyclonal Ab...
    Human Siglec-9 Biotinylated Affinity Purified Pab...
    TUNEL Apoptosis Detection Kit...
    ... NOS activity by monitoring the conversion of ... simple, sensitive, and specific for NOS activity ... and purified enzyme preparations. The kit includes ... reactions. Radiolabeled arginine and NADPH are not ...
    Biology Products: