Navigation Links
Genomic DNA Isolation From 510 mg Marine Invertebrate Tissue

tight pellet. If the protein pellet is not tight, repeat step 3 and include the incubation on ice, then repeat step 4.

DNA Precipitation
1. Pour the supernatant containing the DNA (leaving behind the precipitated protein pellet) into a 1.5 ml microfuge tube containing 300 l 100% isopropanol (2-propanol).

2. Cap the tube and mix the sample by inverting gently 50 times.

3. Centrifuge at 13,00016,000 x g for 1 min; the DNA will be visible as a small white pellet.

4. Pour off supernatant and drain tube briefly on clean absorbent paper. Add 300 l 70% ethanol and invert capped tube several times to wash the DNA pellet.

5. Centrifuge at 13,00016,000 x g for 1 min. Carefully pour off the ethanol. Pellet may be loose, so pour slowly and watch pellet to ensure it stays in the tube.

6. Invert and drain the tube on clean absorbent paper and allow to air-dry for 15 min.

DNA Hydration
1. Add 50 l DNA hydration solution (50 l will give a concentration of 100 g/ml if the total yield is 5 g DNA).

2. Allow DNA to rehydrate overnight at room temperature. Alternatively, heat at 65C for 1 hr. Tap tube periodically to aid in dispersing the DNA. Store DNA at 28C.


back to top
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. New Protocols for Isolating High- Molecular-Weight Genomic DNA
3. Easily Amplify Genomic DNA with Long-Distance PCR
4. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
5. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
6. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
7. Mouse Tail Genomic DNA Isolation Protocol(1)
8. Genomic DNA Isolation Protocol(1,2,3)
9. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
10. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
11. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Genomic DNA Isolation From Marine Invertebrate Tissue

(Date:7/2/2008)...to Advance Commercialization of Expanded Portfolio...ts, VANCOUVER, British Columbia, July 2 /PRNewswi...Ventures Corp. (TSXV: MEV), a company developing a..., today announced its name,change and the expansio...ed,the acquisition of two vascular product develop...
(Date:7/2/2008)..., China, July 2 /Xinhua-- WuXi Pharma...nology and medical device research,and development...the United,States, today announced that it has app...urces., (Logo: http://www.newscom.com/cgi-bin/pr...PharmaTech, Mr. Zhu worked as Head of Asia Human,R...
(Date:7/1/2008)... Mass. (July 1, 2008) Adult cells of mice create...uced pluripotent stem (IPS) stem cellscan be trigg... state, without the need for further genetic alter...new efficiencies to embryonic stem cell research, ...Nature Biotechnology . , "This technical advance...
(Date:7/1/2008)...ILL, N.C., July 1 /- To effectively m...surveys garnered the highest,rating of 3.93 out of...es,in a member-driven research study conducted by ..., However, only 35 percent of companies use this ...lth care segment not using follow-up CSR phone,sur...
Breaking Biology Technology:Medical Ventures Becomes Neovasc with Two Corporate Acquisitions and $8.3 Million Financing 2Medical Ventures Becomes Neovasc with Two Corporate Acquisitions and $8.3 Million Financing 3Medical Ventures Becomes Neovasc with Two Corporate Acquisitions and $8.3 Million Financing 4WuXi PharmaTech (NYSE: WX) Appoints Mr. Bob Zhu as VP of HR 2New technique produces genetically identical stem cells 2Follow-up Phone Surveys: Effective Customer Service Assessment Tool for Improving Satisfaction 2
...riend Marie the other day: Thats it! This makes th...to have it be bought, and see my job go away. Im d...imate notwithstanding, people are still leaving th...r own (and women in greater numbers than men). May...consultant you would make. Maybe you would! But is...
...ity...promotes entertainment everywhere on any dev...nesday shareholder approval for the sale of the co... to Sony Pictures Digital. (SPD) The transaction i...e Vice President Patrick Kennedy has stated that i...and in fact will begin recruiting 10-12 more engin...
...vive in 2003 has an unambiguous mission statement:... Funding and resources are strained to the limit, ...remaining) personnel are having to do the jobs of ...ore with less; is it possible under these conditio...ficulties of 2003 an opportunity for competitive a...
... installment of an interview with Carl Gulbrandse...earch Foundation (WARF) , Gulbrandsen shares his p... university technology-transfer organizations. He ...University of Wisconsin-Madison and he cites examp...d patents WARF has licensed. , ,The next installme...
Other Biology Technology:Is Now the Time to Go on Your Own? 2Is Now the Time to Go on Your Own? 3Sony Completes Acquisition of Sonic Foundry 2Sony Completes Acquisition of Sonic Foundry 3Mission Statement 2003: Do More with Less and Do it Faster! 2Mission Statement 2003: Do More with Less and Do it Faster! 3WTN Interview: Carl Gulbrandsen
WARF - A Unique and Successful Technology Transfer Organization 2WTN Interview: Carl Gulbrandsen
WARF - A Unique and Successful Technology Transfer Organization 3WTN Interview: Carl Gulbrandsen
WARF - A Unique and Successful Technology Transfer Organization 4
(Date:7/2/2008)...y and the U.K. have discovered that our own body n...tive ingredient in marijuana (THC), but these pla...This finding on "endocannabinoids" just published ...t issue of, The FASEB Journal could lead to new ... to dry skin, and even skin-related tumors. , "...
(Date:7/2/2008)...- Two Texas A&M University researchers have develo... more accurately study complex units of clustered ...h allows scientists to analyze many bacterial geno...s because it starts from experimentally validated ... say. The researchers hope their tool will lead to...
(Date:7/2/2008)... want to produce a lot of a substance -- such as a...ts, molecules that speed chemical reactions. Many ...ding one in just the right shape to connect with c...ts, such as enzymes in the human body that help us...fting to suit the task at hand. , Chemists have ...
(Date:7/2/2008)... Spanish . , Most of the functions performed by...teins, which recognise their binding partner by af...There are many kinds of interactions; however, the... structural biology are those which are transient....ugh a large section of the protein surface- the gl...
Breaking Biology News(10 mins):Texas A&M researchers develop tool to study complex clusters of genes 2Texas A&M researchers develop tool to study complex clusters of genes 3Synthetic molecules emulate enzyme behavior for the first time 2Synthetic molecules emulate enzyme behavior for the first time 3Scientists reveal the key mechanisms for affinity between transient binding proteins 2American Society for Microbiology honors Hung Ton That 393 1Gene Mutations May Cause Rare Neonatal Diabetes 1259 1Gene Mutations May Cause Rare Neonatal Diabetes 1259 2DC Psoriasis Event Saturday Will Celebrate First National Short Sleeve Day 1256 1DC Psoriasis Event Saturday Will Celebrate First National Short Sleeve Day 1256 2Vida Health Communications Inc to Be Honored as Tibbetts Award Winner 1253 1Vida Health Communications Inc to Be Honored as Tibbetts Award Winner 1253 2
...etermined that Titanis walleri, a prehistoric 7-fo... America from South America long before a land bri...ist Bruce MacFadden said his team used an establis... elements in a new application to revise the ages ...nly places in North America where the species has ...
...tudy of hepatitis C virus by devising a novel viru...t-isolated virus in nontransformed hepatocytes, in...med cell lines. The related report by Lázaro et a...nd serum-infected cultured human fetal hepatocytes...ournal of Pathology. , Hepatitis C virus (HCV) inf...
... Dr. Susan George and Dr. Brian O,Dowd at the Cent...red a distinct dopamine signalling complex in the ...e receptors, this novel target may have a signific...ia. , Published in the Proceedings of the National...is important discovery demonstrates the existence ...
...deadly prion diseases by smashing up clusters of t...t allow fatal brain illnesses to quickly spread, n... are exciting, researchers say, because they might...ugh drug intervention. If a drug could be made tha...bstantially slow the spread of prions, which cause...
Other Biology News:'Terror bird' arrived in North America before land bridge, study finds 2New culture method for hepatitis C virus uses primary hepatocytes and patient serum 2New dopamine brain target discovered 2Brown team finds crucial protein role in deadly prion spread 2
Request Info
...latypus Contact Printing Kit contains choice of Pr...0/1000 Angsroms of Gold over Ti adhesion layer, 1 ...ver TI adhesion layer, or 3 plain glass slides), P...es of 100 / 200 micron squares or 100 um x 10 mm o...
Melanoma Antigen, Family C, 1 (MAGEC1) Rabbit anti-Human Polyclonal Antibody Immunogen: Synthetic peptide - KLH conjugated Family: New Recommended Storage: Long term: -20C; Short term: +4
OMIX Pipette Tips C18 from Varian, Inc.
Biology Products: