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Genomic DNA Isolation From 1 ml of Body Fluid

transfer supernatant using a pipet so that particulates are excluded. If the DNA yield is expected to be low (<20 g), add a DNA carrier such as glycogen (10 l of 20 mg/ml glycogen per 6 ml isopro p a n o l ) .

2. Mix the sample by inverting gently 50 times and keep tube at room temperature for at least 5 min.

3.Centrifuge at 2,000 x g for 10 min. The DNA may or may not be visible as a small white pellet, depending on yield.

4. Pour off the supernatant and drain tube briefly on clean absorbent paper. Add 6 ml 70% ethanol and invert the capped tube several times to wash the DNA pellet.

5. Centrifuge at 2,000 x g for 1 min. Carefully pour off the ethanol. Pellet may be loose, so pour slowly and watch pellet to ensure it stays in the tube.

6. I n v e rt and drain the tube on clean absorbent paper and allow to air- d ry for 15 min.

DNA Hydration
1. Add 100 l of DNA hydration solution (100 l will give a concentration of 200 g/ml if the yield is 20 g DNA).

2. Allow DNA to rehydrate overnight at room temperature . A l t e rn a t i v e l y, heat at 65C for 1 hr. Tap tube periodically to aid in dispersing the DNA.

3. Vo rtex briefly and pulse-spin before use. Store DNA at 28 C .


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