Navigation Links
Genomic DNA Isolation From 1 ml of Body Fluid


AquaPure Genomic DNA Isolation Kit
Catalog #732-6340
Expected yield range: 250 g DNA (15-ml tube prep)


Method
Cell Lysis
1. Add 1 ml body fluid (e.g., cerebrospinal fluid, plasma, saliva, serum, sputum, synovial fluid, urine, whole blood, milk) to a sterile 15 ml centrifuge tube containing 5 ml cell lysis solution. Pipet up and down several times to mix thoroughly. Note: if the sample has a high protein content, 0.5 ml body fluid may be added to 5.5 ml cell lysis solution.

2. Heat to 65C for 15 min to complete lysis. Alternatively, for maximum yield, add 30 l of proteinase K (20 mg/ml) and incubate lysate at 55C for 1 hr to overnight.

RNase Treatment (Optional)
1. Add 30 l RNase A solution to the cell lysate.

2. Mix the sample by inverting the tube 25 times and incubate at 37C for 1560 min.

Protein Precipitation
1. Cool sample to room temperature.

2. Add 2 ml protein precipitation solution to the lysate.

3. Vortex sample at high speed for 20 sec to mix the protein precipitation solution uniformly with the lysate.

4. Place sample into an ice bath for 515 min.

5. Centrifuge at 2,000 x g for 10 min. The precipitated proteins should form a tight pellet. Note: if body fluid has a high lipid content, particulates may stay near top of tube; see alternative transfer method in step 1 below.

DNA Precipitation
1. Pour the supernatant containing the DNA (leaving behind the p recipitated protein pellet) into a clean 15 ml centrifuge tube containing 6 ml 100% isopropanol (2-propanol). Altern a t i v e l y, if p a rticulates are present,
'"/>

Source:


Page: All 1 2

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. New Protocols for Isolating High- Molecular-Weight Genomic DNA
3. Easily Amplify Genomic DNA with Long-Distance PCR
4. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
5. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
6. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
7. Mouse Tail Genomic DNA Isolation Protocol(1)
8. Genomic DNA Isolation Protocol(1,2,3)
9. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
10. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
11. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Genomic DNA Isolation From Body Fluid

(Date:9/30/2014)... Applying a well-known optical phenomenon called thin-film interference, ... demonstrated the ability to "paint" ultra-thin coatings onto ... for making future, flexible electronic devices, creating advanced ... rocket ships and spacecraft with extremely lightweight decorative ... water, it becomes iridescent, revealing a myriad of ...
(Date:9/30/2014)... analog of one of nature,s most efficient light-harvesting ... led by Alejandro Briseno of the University of ... developing long-sought polymer architecture to boost power-conversion efficiency ... devices. , Briseno, with colleagues and graduate ... University and Dresden University of Technology, Germany, report ...
(Date:9/30/2014)... YORK , Sept. 30, 2014 ... and segments with highest potential revenues Do you ... updated report gives you revenue predictions for those ... For those therapies you find financial data, R&D ... analysis you get sales forecasts to 2024 at ...
(Date:9/30/2014)... NEW YORK , Sept. 30, 2014 /PRNewswire/ ... used to kill nematodes. Nematodes are microscopic parasitic ... soil, water, and inside other plants and animals. ... high volatility or other properties promoting migration through ... contain more than 1,000 plant-parasitic nematodes. With more ...
Breaking Biology Technology:Taking thin films to the extreme 2Blades of grass inspire advance in organic solar cells 2Blades of grass inspire advance in organic solar cells 3Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 2Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 3Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 4Biosimilars and Follow-On Biologics: World Industry and Market Prospects 2014-2024 5Nematicides Market by Types (Fumigants, Organophosphates, Carbamate and Bio-Nematicides), Applications (Agrochemicals, Industrial, and Others), and Geography (North America, Europe, Asia-Pacific & ROW) - Global Trends & Forecasts to 2019 2
... PARK, N.C. - May 25, 2011 - ... consortium,for semiconductors and related technologies, and researchers ... and Applied Science have,developed a new method ... The advance promises to provide,semiconductor chip manufacturing ...
... the wake of the March 11 earthquake and rolling ... Japan, the award-winning undergraduate internship program NanoJapan is scrambling ... than having U.S. undergraduates travel to Japan for lab ... opportunity to send 25-30 students to Rice University laboratories ...
... Rules-Based Medicine (RBM), a global leader in ... has achieved a significant milestone in a companion ... a Roche clinical trial, the RBM DiscoveryMAP® platform, ... pattern was identified that has utility in prognosis ...
Cached Biology Technology:SRC and UCLA advance design-dependent process monitoring for semiconductor wafer manufacturing 2Reverse NanoJapan: Rice to host 25-30 Japanese students 2Rules-Based Medicine Reaches a Significant Milestone in Companion Diagnostic Program With Roche 2
(Date:9/30/2014)... professor and Howard Hughes Medical Institute investigator, and ... recipients in the 2014 round of grants from ... BRAIN (Brain Research through Advancing Innovative Neurotechnologies) Initiative ... September 30, provides more than $3 million in ... , The BRAIN Initiative, launched last year, is ...
(Date:9/30/2014)... news release is available in German . ... are among the most expensive foods in the world. Because ... find them. But the distinctive smell of truffles is not ... French scientists under the direction of the Goethe University Frankfurt ... produced by soil bacteria which are trapped inside truffle fruiting ...
(Date:9/30/2014)... trouble getting pregnanteven with IVF? Here,s some hope: A ... The FASEB Journal , explains how ... known as PAWP, which induced embryo development in human ... embryo development by the sperm cell during fertilization. , ... a major paradigm shift in our understanding of human ...
Breaking Biology News(10 mins):Salk scientists receive $3 million for BRAIN Initiative grant 2On the trail of the truffle flavor 2Synthetic sperm protein raises the chance for successful in vitro fertilization 2
... Researchers at Ben-Gurion University of the Negev (BGU) have discovered ... the possibility for diseases, including cancer., The findings were recently ... The team of researchers from BGU,s National Institute for Biotechnology ... in the genome of the mitochondria, a part of every ...
... N.C. New research out of Wake Forest University ... solid scientific answer for the long-standing question of whether ... malformations. In a study of more than ... a strong association between congenital brain defects and preterm ...
... and a domain unto themselves, quite apart from the ... species live under extreme conditions, and carry out unique ... Methanogenic archaeans, for example, can produce methane gas out ... a reduction, involves the cofactor known as F0 or ...
Cached Biology News:Ben-Gurion U. researchers reveal connection between cancer and human evolution 2Brain malformations significantly associated with preterm birth, Wake Forest research shows 2Brain malformations significantly associated with preterm birth, Wake Forest research shows 3Rampant helper syndrome 2
... Certify products ATP-Free using Mo Bio ... sensitive ATP-Free certification service that will allow you ... level of accuracy and enable you to deliver ... Mo Bios ATP detection limit is 3.5 ...
... Microarrays: Biomedical Photometrics Inc. (Waterloo, Ontario, Canada) ... have collaborated to develop a fluorescence reader ... The DNAscope AT is based on BPIs ... automatically reads up to six ArrayTubes in ...
... a comprehensive kinase analysis service utilizing ... peptide microarrays synthesized on Paraflo microfluidic ... quantitative measurement of kinase kinetic activities, ... service includes preparation of your kinase ...
... The JNK (c-Jun NH2-terminal kinase) ... three identified MAP kinase pathways in ... response to environmental stress, is associated ... itself, and is implicated in the ...
Biology Products: