Navigation Links
Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood ,,, Mini Kit

Laura Pollock, Jennifer Halcome, and Michele Nelson Hurst
Eppendorf 5 Prime, Inc. Abstract

The Perfect gDNA Blood Mini Kit is an efficient way to purify genomic DNA from 200 l of whole blood as described in the product manual. In addition, it has been shown that the kit can also be used to effectively purify genomic DNA from buffy coat after the whole blood has been separated into its components. Using this method provides obvious benefits to the researcher because the gDNA isolated from buffy coat is higher in concentration and yield than the corresponding whole blood sample. Best results are found when an entire vial of blood is separated into its component parts, but it has also been proven that the buffy coat, from as little as 1.5 ml of whole blood, gives good results.

Introduction

The versatile Perfect gDNA Blood Mini Kit allows the researcher to isolate gDNA from a variety of biological samples, including buffy coat. The purification method is based on Proteinase K lysis followed by collection of the crude high molecular weight gDNA on a membrane. The gDNA is then washed to remove contaminants and eluted in a buffer suitable for common downstream applications such as PCR and restriction digest. The method is spin-column-based and simply requires the use of a standard microcentrifuge. No additional purification is required.

Buffy coat is the component of whole blood that contains leukocytes and platelets and makes up only 1% of the entire whole blood composition. Separating out this particular component enables the researcher to enrich the DNA-containing leukocytes and
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. New Protocols for Isolating High- Molecular-Weight Genomic DNA
3. Easily Amplify Genomic DNA with Long-Distance PCR
4. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
5. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
6. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
7. Mouse Tail Genomic DNA Isolation Protocol(1)
8. Genomic DNA Isolation Protocol(1,2,3)
9. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
10. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
11. Genomic DNA Isolation Protocol(1,2,3)

Post Your Comments:
(Date:11/19/2009)...THESDA, Md., Nov. 19 Sph...n biotechnology for diabetes therapy, and a provid... to food, supplement, biotechnology and pharmaceut...sday,s Annual Meeting of Shareholders. ,, At th...asdaq: SPEX ) elected the following members to th...
(Date:11/19/2009)... Top LASIK surgeons and recognized ref...sted LASIK Surgeons made brought their fellow surg...on correction surgery, cataract surgery, and ophth...ican Academy of Ophthalmology (AAO) annual meeting...om, Dr. Shachar Tauber, Dr. David Huang, MD, Dr. R...
(Date:11/18/2009)...IS, Calif., Nov. 18 Marrone Bio In...pment and commercialization of effective and envir...nnounced that it has been named an IC20 company by...and largest national angel investing group. ,, ...eading to rewarding results for all stakeholders, ...
(Date:11/18/2009)...at Queen,s University Belfast are developing new s... which will help in the fight against the threat o...ial gel pads to ,swipe, an individual or crime sce...scanning instrument that can detect the presence o..., faster decisions to be made in response to terro...
Breaking Biology Technology:Spherix Announces Results of Annual Shareholders Meeting 2Spherix Announces Results of Annual Shareholders Meeting 3Spherix Announces Results of Annual Shareholders Meeting 4Experts Listed at Trusted LASIK Surgeons Bring Peers Up to Date on Vision Correction and Cataract Surgery at Annual AAO Meeting 2Experts Listed at Trusted LASIK Surgeons Bring Peers Up to Date on Vision Correction and Cataract Surgery at Annual AAO Meeting 3Experts Listed at Trusted LASIK Surgeons Bring Peers Up to Date on Vision Correction and Cataract Surgery at Annual AAO Meeting 4Experts Listed at Trusted LASIK Surgeons Bring Peers Up to Date on Vision Correction and Cataract Surgery at Annual AAO Meeting 5Experts Listed at Trusted LASIK Surgeons Bring Peers Up to Date on Vision Correction and Cataract Surgery at Annual AAO Meeting 6Experts Listed at Trusted LASIK Surgeons Bring Peers Up to Date on Vision Correction and Cataract Surgery at Annual AAO Meeting 7Experts Listed at Trusted LASIK Surgeons Bring Peers Up to Date on Vision Correction and Cataract Surgery at Annual AAO Meeting 8Experts Listed at Trusted LASIK Surgeons Bring Peers Up to Date on Vision Correction and Cataract Surgery at Annual AAO Meeting 9Marrone Bio Innovations Named an IC20 Company 2New technology detects chemical weapons in seconds 2
...Wis. Information technology professionals might ...ifferent language. That,s what an expert panel at ...ave some tips on simplifying tech-speak for execut... can hold sway in the boardroom. , ,Good IT conver...aking, said John Byrnes, managing director of the ...
...tive Web site requires an ongoing investment, but ...ou. , ,Every Web site needs to provide a tangible ...y,s Web site should be one of the most active and ...ability is a good thing as long as it,s based on ...tangible things such as cost savings and intangibl...
... Company does a great job of tracking U.S. biotec... funds flowing through due to partnerships with ot...cent analysis, it would appear that 2004 was a ver...g categories. Lets take a look: , , , ,If 2003 w...e biotech sector from a variety of different sourc...
Other Biology Technology:CIOs learn boardroom-speak 2CIOs learn boardroom-speak 3How hard is your Web site working? 2How hard is your Web site working? 3How hard is your Web site working? 42004 a banner year for biotech financing 2
(Date:11/20/2009)... in the November 2009 issue of the journal GENETI...ow a family of genes (1-aminocyclopropane-1-carbox...production of ethylene. This gas affects many aspe...s the foundation for future genetic manipulation t...urvive and thrive in difficult terrain, increase y...
(Date:11/20/2009)...nts of plasma lipoprotein concentrations determine..., researchers led by Daniel Chasman at Brigham and...ston, MA, the Framingham Heart Study in Framingham...n and Oxford, England performed genetic associatio...men of European ancestry from the Women,s Genome H...
(Date:11/20/2009)...tribute their abstracts to the World Congress on O...lobal meeting devoted to all aspects of osteoporos...he Congress will be held in Florence, Italy from M...e submitted online at http://www.iofwco-ecceo10.o...10. , IOF and ESCEO are pleased to announce that...
Breaking Biology News(10 mins):It's a gas: New discovery may lead to heartier, high-yielding plants 2Genetic analysis helps dissect molecular basis of cardiovascular disease 2Special sessions announced for World Congress on Osteoporosis 2010 - IOF WCO - ECCEO10 2Special sessions announced for World Congress on Osteoporosis 2010 - IOF WCO - ECCEO10 3Ohio Supreme Court Supports Regulation of Dangerous Abortion Drug 50820 1Ohio Supreme Court Supports Regulation of Dangerous Abortion Drug 50820 2Antidepressants aid electroconvulsive therapy in treating severe depression 50818 1Antidepressants aid electroconvulsive therapy in treating severe depression 50818 2NCI Renews Mayo Clinic Cancer Center Support Grant Extends Comprehensive Status 50816 1NCI Renews Mayo Clinic Cancer Center Support Grant Extends Comprehensive Status 50816 2
...plants, ability to sprout upward through the air, ... long been considered one of the characteristics s...water to support them. , Now lignin, one of the ...g nature of land plants and thought unique to the...earchers including scientists at UBC and Stanford ...
...niversity of Houston,s commitment to green initiat...ica presented UH President Renu Khator with an env...t of a $300,000 donation to the university in supp...oud to support the University of Houston,s enginee...of its vital efforts to attract and train the stud...
... is no good evidence supporting a harmful effect o...ts and regular exercise, according to a review of ...urnal of Anatomy . , Exercise is an extremely pop...ghout the Western world and has for many become pa...in as being beneficial for weight control, disease...
Other Biology News:Billion-year revision of plant evolution timeline may stem from discovery of lignin in seaweed 2$300,000 donation from BP includes solar vehicle for UH 2
Taq DNA Polymerase, 500 U (2 x 250 U) without dNTP from Bioneer, Inc.
iTaq DNA Polymerase, 5,000 U from Bio-Rad
Rabbit Anti-hnRNP-U Polyclonal Antibody, Unconjugated from Novus Biologicals
iTaq DNA Polymerase, 250 U from Bio-Rad
Biology Products: