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Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood ,,, Mini Kit

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Results and Discussion

gDNA from all three buccal swabs performed well in amplification of both the 526 bp and 2 kb human beta-globin fragments (see Fig. 1). gDNA isolated from buccal swabs using the Perfect gDNA Blood Mini Kit works well in the downstream application of PCR. Isolation of sufficient amounts of gDNA is critical for PCR. Buccal swabs provide a less invasive method for obtaining DNA-containing samples. The Eppendorf Perfect gDNA Blood Mini Kit enables the researcher to obtain these small but adequate amounts of DNA to be used in PCR. Overall, the Eppendorf Perfect gDNA Blood Mini Kit provides a fast, easy and effective method for purification of gDNA from buccal swabs.
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References
  1. Use of the Polymerase Chain Reaction (PCR) process to amplify nucleic acids is covered by U.S. Patent Numbers 4683105 and 4683202 owned by Roche Molecular and requires a license.
Fig. 1: PCR of gDNA from buccal swabs isolated using the Perfect gDNA Blood Mini Kit. 1% agarose gel in 1x TAE stained in ethidium bromide 10 l of the 30 l reaction was loaded.

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