Navigation Links
Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood ,,, Mini Kit

ide of a donors cheek 6 times. The donor should not consume food or drink 30 minutes prior to sampling.
  • Remove the end of the swab by slicing or cutting and place in a 2 ml microcentrifuge tube. Add 400 l of 1x PBS to the tube.
  • Add 20 l of Proteinase K and 350 l of Solution G1 to the tube containing the swab. Vortex vigorously for 5 seconds.
  • Incubate at 70C, 900 rpm for 10 minutes in a Thermomixer. A water bath may be used with intermittent vortexing of the sample if a Thermomixer is not available.
  • Carefully transfer the liquid only to a new tube. Add 200 l Solution G2. Vortex the sample on high for 5 seconds. Place a spin column in a fresh microcentrifuge tube. Transfer the sample to the spin column assembly. Incubate the sample at room temperature for 1 minute.
  • Centrifuge the sample for 2 minutes at 12,00016,000 x g. Remove the spin column and decant flow-through. Place the spin column back into the same tube.
  • Add 600 l Diluted Wash Buffer to the spin column. Centrifuge for 1 minute at 12,00016,000 x g. Remove the spin column and decant flow-through. Place the spin column back into the same tube.
  • Add 400 l Diluted Wash Buffer to the spin column. Centrifuge for 3 minutes at 12,00016,000 x g. Carefully remove the spin column without splashing Wash Buffer onto the bottom of the column. Place the spin column into a fresh microcentrifuge tube.
  • Observe the filter membrane to make sure it is dry before proceeding. If the filter looks shiny, spin an additional 1 to 2 minutes before placing the spin column into a
    '"/>


  • Source:


    Page: All 1 2 3 4 5

    Related biology technology :

    1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
    2. New Protocols for Isolating High- Molecular-Weight Genomic DNA
    3. Easily Amplify Genomic DNA with Long-Distance PCR
    4. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
    5. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
    6. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
    7. Mouse Tail Genomic DNA Isolation Protocol(1)
    8. Genomic DNA Isolation Protocol(1,2,3)
    9. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
    10. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
    11. Genomic DNA Isolation Protocol(1,2,3)
    Post Your Comments:
    (Date:5/1/2015)... May 1, 2015 A new ... antithrombotic drugs will reach $22.4bn in 2019. That revenue ... Industry and Market Prospects 2015-2025 ,  published ... investigation is to provide forecasts and qualitative analyses of ... publisher and consultancy in London, UK ...
    (Date:5/1/2015)... DALLAS , May 1, 2015 ... report "Antibody Production Market by Product (Equipment, Consumables), by ... (Biopharmaceutical Companies, Diagnostic Labs, Research Institutes) - Analysis ... the report studies the global Antibody Production Market ... This market is expected to reach $2.572 Billion ...
    (Date:4/30/2015)... , April 30, 2015  Roka Bioscience, Inc. (NASDAQ: ... providing advanced testing solutions for the detection of foodborne ... the first quarter of 2015 on May 7, 2015 ... Roka Bioscience will also host a conference call at ... discuss its first quarter financial results. During the conference ...
    (Date:4/30/2015)... /PRNewswire/ - BioAmber Inc. (NYSE: BIOA ), an ... has commenced an underwritten public offering of its common ... a 30-day option to purchase up to an additional ... in the public offering. The offering is subject to ... to whether or when the offering may be completed, ...
    Breaking Biology Technology:Antithrombotic Drugs: World Industry and Market Prospects 2015-2025 2Antithrombotic Drugs: World Industry and Market Prospects 2015-2025 3Antithrombotic Drugs: World Industry and Market Prospects 2015-2025 4Antithrombotic Drugs: World Industry and Market Prospects 2015-2025 5Antibody Production Market worth $2.572 Billion by 2019 2Antibody Production Market worth $2.572 Billion by 2019 3Antibody Production Market worth $2.572 Billion by 2019 4Roka Bioscience to Report First Quarter 2015 Financial Results On May 7, 2015 2BioAmber Inc. Announces Public Offering Of Common Stock 2BioAmber Inc. Announces Public Offering Of Common Stock 3
    ... Feb. 8 MBS Value Partners, a strategic ... domestic and international clients, announced today that Lisa Lettieri ... Directors. , “Lisa and Matt bring outstanding senior-level advisory ... Lynn Morgen , co-founder of MBS. “Lisa’s and Matt’s ...
    ... Healthcare Data Solutions ... expanding staff of healthcare and data experts. Ms. Wichman joins HDS as an Account Manager and ... as the Manager of that group Omaha, NE. , ... Foothill Ranch, CA (Vocus) February 8, 2010 -- Healthcare Data Solutions (HDS) ...
    ... of Maryland researchers have developed and successfully tested ... patterns of improvised explosive device (IED) attacks in ... locations of weapons caches that are used by ... Maryland computer science Ph.D. student Paulo Shakarian and ...
    Cached Biology Technology:MBS Value Partners Hires Two New Senior Staff Members 2Healthcare Data Solutions Announces Expansion of its Healthcare Customer Solutions Team With Two Key Additions 2Healthcare Data Solutions Announces Expansion of its Healthcare Customer Solutions Team With Two Key Additions 3'Fighting' IED attacks with SCARE technology 2'Fighting' IED attacks with SCARE technology 3
    (Date:3/23/2015)... March 23, 2015   HOYOS Labs , a ... today announced that the Company will demonstrate multiple biometrics-based ... and consumers at Connect:ID on March 23 through 25, ... Labs will highlight the IEEE Biometric Open Protocol Standard; ... and enterprise access control system. BOPS was invented by ...
    (Date:3/23/2015)... , Mar. 23, 2015 NXT-ID, Inc. (NASDAQ: NXTD ... on the growing mobile commerce market, announces its biometric payment ... campaign on CNBC television starting March 30 th . ... airing in New York markets. ... "We are excited about our new ad campaign following the ...
    (Date:3/20/2015)... , Mar. 19, 2015 Research and Markets ... "Hand Geometry - Global Strategic Business Report" report ... markets for Hand Geometry in US$ Thousands. The report provides ... , Japan , Europe ... , and Rest of World. Annual estimates ...
    Breaking Biology News(10 mins):HOYOS Labs to Showcase Host of Biometrics Technologies at Connect:ID in Washington, D.C. 2HOYOS Labs to Showcase Host of Biometrics Technologies at Connect:ID in Washington, D.C. 3NXT-ID's Wocket Smart Wallet to Launch New CNBC Regional TV Ad Campaign 2NXT-ID's Wocket Smart Wallet to Launch New CNBC Regional TV Ad Campaign 3Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 2Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 3Hand Geometry - Global Strategic Business Report 2015: An Essential Security Component for both Government & Enterprise Sector 4
    ... exercise speeds learning and improves blood flow to the brain, ... University of Pittsburgh School of Medicine that is the first ... findings are available in the journal Neuroscience . ... exercise on cognition in other animal models, such as the ...
    ... GRAND RAPIDS, MICH., April 22, 2010 Topping ... or sauting kale instead of spinach for dinner ... for certain chronic diseases, including cardiovascular disease, cancer and ... and presented at the Experimental Biology Meeting, April 25, ...
    ... Breastfeeding is best, but what happens when something goes ... this "natural" process even after carefully following all the ... Not surprisingly, some women have more difficulty than others ... problems especially in the first week after birth. ...
    Cached Biology News:Regular aerobic exercise is good for the brain, Pitt team says 2Regular aerobic exercise is good for the brain, Pitt team says 3New research suggests choosing different fruits and vegetables may increase phytonutrient intake 2New research suggests choosing different fruits and vegetables may increase phytonutrient intake 3New research suggests choosing different fruits and vegetables may increase phytonutrient intake 4Clinical study supports benefit of breastfeeding support for obese women 2Clinical study supports benefit of breastfeeding support for obese women 3
    Human sVCAM-1/CD106 ELI-PAIR Kit...
    HP 1 alpha Immunogen: Synthetic peptide between amino acids 1-100 of the human HP 1 alpha protein. Storage: -20 C, Avoid Freeze/Thaw Cycles...
    ... include subcloning of gene in baculoviral ... isolation and plaque purification of ten ... stock for one clone, protein expression ... cell culture. Expected yield and timeline: ...
    ... Synthesis Service provides you with ... knockdown any gene. We offer ... pairs with terminal dT nucleotides), ... lengths of your choice. Each ...
    Biology Products: