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Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood ,,, Mini Kit

ide of a donors cheek 6 times. The donor should not consume food or drink 30 minutes prior to sampling.
  • Remove the end of the swab by slicing or cutting and place in a 2 ml microcentrifuge tube. Add 400 l of 1x PBS to the tube.
  • Add 20 l of Proteinase K and 350 l of Solution G1 to the tube containing the swab. Vortex vigorously for 5 seconds.
  • Incubate at 70C, 900 rpm for 10 minutes in a Thermomixer. A water bath may be used with intermittent vortexing of the sample if a Thermomixer is not available.
  • Carefully transfer the liquid only to a new tube. Add 200 l Solution G2. Vortex the sample on high for 5 seconds. Place a spin column in a fresh microcentrifuge tube. Transfer the sample to the spin column assembly. Incubate the sample at room temperature for 1 minute.
  • Centrifuge the sample for 2 minutes at 12,00016,000 x g. Remove the spin column and decant flow-through. Place the spin column back into the same tube.
  • Add 600 l Diluted Wash Buffer to the spin column. Centrifuge for 1 minute at 12,00016,000 x g. Remove the spin column and decant flow-through. Place the spin column back into the same tube.
  • Add 400 l Diluted Wash Buffer to the spin column. Centrifuge for 3 minutes at 12,00016,000 x g. Carefully remove the spin column without splashing Wash Buffer onto the bottom of the column. Place the spin column into a fresh microcentrifuge tube.
  • Observe the filter membrane to make sure it is dry before proceeding. If the filter looks shiny, spin an additional 1 to 2 minutes before placing the spin column into a
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