Navigation Links
Genomic Analysis of Formalin-Fixed Paraffin Embedded (FFPE) Tissues through the use of Whole Genome Amplification (WGA)

ncy, giving rise to some loss of representation of DNAs in this size range. Genomic DNA fragments less than ~400 bp will not amplify at all using GenomePlex. (18). Highly fragmented DNA (<150 bp) will, when subjected to the GenomePlex methodology, self prime to create product with very low representation as compared to the original sample.

Amplification limitations are not the only obstacle in analyzing formalin treated samples. Studies have shown that formalin treatment weakens DNA, making it more susceptible to shearing when subjected to column based bind and elute purification.4 The most recent GenomePlex offering, (Cat. No. WGA5), designed for fresh, frozen or fixed tissues, includes reagents to effect a very gentle extraction. The lysis uses a combination of proteinase K and lytic detergent, and draws from the experience gained in the development of the Sigma Extract-N-Amp reagent line. Efficient, gentle lysis and a robust amplification should give the greatest possible chance for the researcher to amplify their tissue-derived DNA.

In conclusion, WGA from fixed tissues is a very challenging, but rich source of potential genetic information. Because GenomePlex initially generates a library of amplifiable template molecules by relying upon a simple degenerate primer extension, it alone is poised to amplify DNAs that are highly fragmented and/or modified. The DOP amplification mechanism and the gentle lysis included in the GenomePlex Tissue WGA kit make it the best commercial choice for these difficult substrates.


References
1. Impraim, C., et al., (1987) Analysis of DNA extracted from formalin-fixed, paraffinembedded tissues by enzymatic amplification and hybridization with sequence-specific oligon
'"/>

Source:


Page: All 1 2 3 4 5 6 7

Related biology technology :

1. Fast and Easy Isolation of PCR-Ready Genomic DNA from Whole Blood
2. New Protocols for Isolating High- Molecular-Weight Genomic DNA
3. Easily Amplify Genomic DNA with Long-Distance PCR
4. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
5. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
6. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
7. Mouse Tail Genomic DNA Isolation Protocol(1)
8. Genomic DNA Isolation Protocol(1,2,3)
9. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
10. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
11. Isolation of Genomic DNA from Saliva Using the Perfect gDNA Blood Mini Kit
Post Your Comments:
(Date:7/30/2014)... While administrative claims and other data ... a relatively long period of time, there has ... of these ‘big data’ for comparisons of interventions ... powerful computer technology with the plethora of data ... significant promise for identifying optimal interventions for specific ...
(Date:7/30/2014)... With the accelerated aging of ... expenditure as well as the improved medical care ... reagent industry has been developing quickly, especially in ... Chinese in vitro diagnostics market size reached RMB22.98 ... vitro diagnostic reagents. , Read complete report ...
(Date:7/30/2014)... Yuma, AZ (PRWEB) July 30, 2014 ... , State-of-the-art facility and complementary therapies brings comprehensive ... square foot, state-of-the-art Yuma Regional Cancer Center has ... since 2012, the program includes services such as ... laboratory, massage therapy, American Cancer Society Resource Center, ...
(Date:7/30/2014)...   Epic Sciences, Inc. ("Epic"), a private ... personalize and advance the treatment and management of cancer, ... as chairman of Epic,s board of directors. Mr. ... (formerly Invitrogen). Mr. Lucier joins Epic,s board of directors ... cell analysis platform with special focus on the development ...
Breaking Biology Technology:Leveraging ‘Big Data’ - Methodological Considerations in Health Services Research, New Webinar Hosted by Xtalks 2China Diagnostic Reagent Industry Report, 2013-2016 | Researchmoz 2China Diagnostic Reagent Industry Report, 2013-2016 | Researchmoz 3Sate-of-the-Art Yuma Regional Cancer Center Officially Opens 2Epic Sciences Appoints Greg Lucier as Chairman of the Board of Directors 2Epic Sciences Appoints Greg Lucier as Chairman of the Board of Directors 3
... the University of Helsinki, Finland, has succeeded in producing ... is reduced to a nano-scale (one nanometre being one ... undergo substantial changes. Unlike in bulk materials, in nanoparticles ... the number of atoms inside the material, which, among ...
... Corporation (Amex: ETC ) ("ETC" or ... will be exhibiting at the upcoming APWCA 2009 ... (APWCA) 2009 National Conference will be held April ... Philadelphia, Center City. The APWCA provides cutting-edge education ...
... Portfolio LAKE FOREST, Ill., March 16 ... equity firm focused exclusively on the healthcare ... CorePharma LLC ("CorePharma") has completed the acquisition ... Leucovorin Calcium tablets are the generic equivalent ...
Cached Biology Technology:Paper electrified by copper particles 2Environmental Tectonics Corporation Announces Participation at APWCA National Conference 2Environmental Tectonics Corporation Announces Participation at APWCA National Conference 3RoundTable Healthcare Partners Facilitates Product Acquisition for CorePharma 2
(Date:7/30/2014)... potential effects of climate change on the world,s ... wrong factors, according to a new paper by ... University of Queensland, and other organizations. The authors ... missing the point when it comes to climate ... change scientists focus on the "direct" threats of ...
(Date:7/30/2014)... Society of America (ESA) has elected ten new Fellows ... Fellow acknowledges outstanding contributions to entomology in one or ... The following Fellows will be recognized during Entomology 2014 ... held November 16-19, 2014 in Portland, Oregon. , ... the Department of Plant, Soil and Entomological Sciences at ...
(Date:7/30/2014)... joint venture between The University of Texas at San ... Center at San Antonio, has selected the winners of ... from UTSA and Lei Huang from the Health Science ... engineered ferritins for tracking and protecting neural stem cells ... focused on finding a treatment for stroke through stem ...
Breaking Biology News(10 mins):Conservation scientists asking wrong questions on climate change impacts on wildlife 2Entomological Society of America recognizes 2014 fellows 2Entomological Society of America recognizes 2014 fellows 3Entomological Society of America recognizes 2014 fellows 4Entomological Society of America recognizes 2014 fellows 5Entomological Society of America recognizes 2014 fellows 6Entomological Society of America recognizes 2014 fellows 7Entomological Society of America recognizes 2014 fellows 8Entomological Society of America recognizes 2014 fellows 9Entomological Society of America recognizes 2014 fellows 10Entomological Society of America recognizes 2014 fellows 11Entomological Society of America recognizes 2014 fellows 12Entomological Society of America recognizes 2014 fellows 13Entomological Society of America recognizes 2014 fellows 14Entomological Society of America recognizes 2014 fellows 15Entomological Society of America recognizes 2014 fellows 16Entomological Society of America recognizes 2014 fellows 17
... March 1 issue of G&D, Dr. Christopher Fasano (Memorial ... into the effective generation of neural stem cells outside ... demonstrates that the polycomb group protein, Bmi-1, maintains forebrain ... and embryonic forebrain neural progenitor cells increases stem cell ...
... since insects developed a taste for vegetation, plants have ... their neighbors for light to grow, or, invest directly ... of scientists at the Salk Institute for Biological Studies ... a la Agronoma (IFEVA) has discovered how plants weigh ...
... immunodeficiency virus (HIV) adapts so well to the body,s ... pace with the ever-changing immunological profile of the virus, ... Birmingham (UAB) and the University of Oxford in England. ... adapt by spelling out at least 14 different "escape ...
Cached Biology News:Understanding natural crop defenses 2Understanding natural crop defenses 3HIV adapts to 'escape' immune response 2
Request Info...
Form: Ready to use Applications: ELISA...
... supports that retain stiffness in liquids, protect ... segments on each strip have chemically bonded ... charts provide clear comparison. Full-range strips (0 ... packs of 100; each strip is approximately ...
... Bench-Top Open System Chemistry Analyzer for General Chemistries, Specialty Chemistries, QC, Reagent Development, Pharmaceutical ... grating, diode spectrophotometer over 30 user-selectable wavelengths. ... ... ...
Biology Products: