Navigation Links
GenomiPhi amplification of FFPE tissue DNA for array CGH

as also similar between the two methods (data not shown).

Because identical FFPE samples were amplified and labeled with Cy3 and Cy5 dyes, the log2 ratios from GenomiPhi amplified samples clustered close to the baseline level, i.e. 0 (data not shown). While no DNA copy-number changes were measured, previous data published using Phi29-based amplification has shown that GenomiPhi DNA Amplification Kit is highly sensitive in detecting monosomy and trisomy in array CGH (6, 7).


Conclusions
Our results showed that GenomiPhi DNA Amplification Kit can be used to amplify DNA extracted from FFPE tissue. Phi29-based whole-genome amplification, with its high processivity and strand-displacement activity, proved to be a robust method for amplifying DNA extracted from FFPE templates. The amplification yield from the GenomiPhi kit was higher than that from the DOP-PCR method. We also showed that FFPE tissues can be subjected to CGH analysis using samples amplified with GenomiPhi DNA Amplification Kit. Spectral Genomics human BAC arrays were successfully used to test the feasibility of array CGH using the GenomiPhi method. Comparison of hybridization results showed mean signal intensities and signal-to-background ratios of GenomiPhi amplified samples to be similar to those of DOP-PCR. Because GenomiPhi DNA Amplification Kit can amplify DNA from small quantities of template, this method can be used to study copy-number changes in FFPE samples on BAC CGH arrays.


Troubleshooting
Problem
Low yield from DNA extraction from FFPE tissue.

Solution
Use FFPE slides with at least 20 µm of tissue for DNA extraction. Complete the extraction method without a purification step because increased manipulation of the genomic DNA can result in loss of overall yield.

'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. Oragene and Whole Genome Amplification with GenomiPhi
2. GenomiPhi: protocol for BAC amplification and fabrication of slides for array CGH
3. Automation of TempliPhi and GenomiPhi DNA Amplification Kits on the Genesis Freedom Robotic Sample Processor
4. The TripleMaster PCR System for three different amplifications
5. Successful PCR amplification and subcloning of a GC-rich DNA fragment
6. Advantages of Roche Applied Science amplification products
7. Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.
8. Protocol for GenomePlex Whole Genome Amplification from Formalin-Fixed Parrafin-Embedded (FFPE) tissue
9. 2D-LC analysis of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
10. Sensitive identification of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
11. High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: GenomiPhi amplification FFPE tissue DNA for array CGH

(Date:9/18/2014)... , Sept. 18, 2014 Research ... "Global and Chinese Stem Cell Industry Report, 2014-2017" ... cells are undifferentiated biological cells that can differentiate into ... more stem cells. Stem cell therapy can be applied ... hematological system disease), nervous system diseases, damage or lesion ...
(Date:9/18/2014)... 2014 Leatherwood Plastics has selected ... to stay competitive in the semiconductor, clean room ... ultimately impact product quality and productivity, according to ... Established in 1980 in Lewisville, TX, Leatherwood ... have left the business. Much of Leatherwood’s equipment ...
(Date:9/18/2014)... - Oncothyreon Inc. (NASDAQ: ONTY ) today ... underwritten offerings of 10,000,000 shares of its Common Stock ... share, for expected gross proceeds of $20 million and ... at a price to the public of $2,000.00 per ... share of Series A Convertible Preferred Stock is non-voting ...
(Date:9/18/2014)... SeqLL Inc., the owner of ... today the closing of a $1M Series-A funding ... Diagnostic Technologies, will increase the power of tSMS™ ... in March 2013 by Daniel Jones, a former ... BioSciences, SeqLL has continued to offer Helicos’ sequencing ...
Breaking Biology Technology:Global and Chinese Stem Cell Industry Report, 2014-2017 2Global and Chinese Stem Cell Industry Report, 2014-2017 3Leatherwood Plastics Selects Vycom’s Flametec PVC-C Material to Stay Competitive in Semiconductor and Lab Equipment Industries 2Oncothyreon Prices $40 Million Concurrent Public Offerings of Common Stock and Preferred Stock 2Oncothyreon Prices $40 Million Concurrent Public Offerings of Common Stock and Preferred Stock 3SeqLL Closes Series-A Funding Round for Expansion of Single Molecule Sequencing 2
... Dec. 21, 2011 Acumen Pharmaceuticals announces today ... (formerly Merck & Co., Inc.) of a 2003 ... Merck exclusive worldwide rights to develop and commercialize ... Merck,s decision to discontinue this ...
... 21 , 2011  To help emerging and ... and commercial environment, PAREXEL International (NASDAQ: ... services provider, today announced that it is introducing ... and outsourcing leaders worldwide.  During these forums, PAREXEL ...
... Osteologix Holdings, PLC ("Osteologix" or the "Company") ... of record on December 19, 2011.  The payment date will ... to allow time for certain Irish Dividend WithholdingTax ("DWT") exemption ... to dividend payment. Shareholders who are not Irish ...
Cached Biology Technology:Acumen Pharmaceuticals Regains Worldwide Rights to Anti-ADDL Antibody Therapeutics and Diagnostics for Alzheimer's Disease From Merck 2PAREXEL Introduces Executive Briefing Series on Proven Practices to Assist Emerging and Mid-size Biopharma in Achieving Better Outcomes 2PAREXEL Introduces Executive Briefing Series on Proven Practices to Assist Emerging and Mid-size Biopharma in Achieving Better Outcomes 3PAREXEL Introduces Executive Briefing Series on Proven Practices to Assist Emerging and Mid-size Biopharma in Achieving Better Outcomes 4Osteologix Announces Dividend 2Osteologix Announces Dividend 3
(Date:9/19/2014)... SHELTON, Conn. , Sept. 19, 2014 Nxt-ID, Inc. ... a biometric authentication company focused on the growing m-commerce market, updates ... and world champion in the lightweight and light middleweight weight classes, ... Yesterday it was announced that Game of Thrones ... and Married with Children star Katey Sagal ...
(Date:9/19/2014)... regulate their symbiotic relationship with soil bacteria by ... plant structure from leaves into the roots to ... roots. This collaborative study was conducted by researchers ... Graduate University for Advanced Studies (SOKENDAI), and the ... , Legumes, an important plant family which ...
(Date:9/18/2014)... State University researchers have developed a unique method to ... cleanup in rural areas. , The first microbe-powered, ... and quick way to clean up waste from large ... pollution. , Professor Haluk Beyenal and graduate student ... Architecture discuss the system in the online edition of ...
Breaking Biology News(10 mins):NXT-ID's Vinny Paz Celebrity Ad Wocket Update: Katey Sagal and Ciaran Hinds Attached to His Upcoming Movie 2NXT-ID's Vinny Paz Celebrity Ad Wocket Update: Katey Sagal and Ciaran Hinds Attached to His Upcoming Movie 3For legume plants, a new route from shoot to root 2Researchers develop unique waste cleanup for rural areas 2
... pharmaceutical industry is struggling to deal with bacteria that ... is the similar struggle in agribusiness to deal with ... is widely used in farming practice. The herbicide, ... in Monsanto,s Roundup products and also in herbicides produced ...
... Mich. Sheng Yang He, plant biologist at Michigan ... most-innovative plant scientists as part of a $75 million ... Medical Institute and the Gordon and Betty Moore Foundation ... Laboratory, and 14 other researchers from around the country. ...
... HOUSTON (June 16, 2011) Researchers at The ... have been awarded nearly $1 million to investigate Adenovirus-36 ... The grant is being awarded by the Federal Emergency ... The study will examine the relationship of Ad-36 to ...
Cached Biology News:Scientists learn how horseweed shrugs off herbicide 2Scientists learn how horseweed shrugs off herbicide 3Scientists learn how horseweed shrugs off herbicide 4Scientists learn how horseweed shrugs off herbicide 5MSU plant scientist named one of the nation's most innovative researchers 2UTHealth awarded FEMA grant to explore novel obesity risk factor among firefighters 2UTHealth awarded FEMA grant to explore novel obesity risk factor among firefighters 3
...
Mouse monoclonal antibody to QPCT - glutaminyl-peptide cyclotransferase (glutaminyl cyclase)...
...
... number is a new product ... Cornings product number. If showing ... under the old Sigma-Aldrich number ... for assistance. ID clarifier: barcoded ...
Biology Products: