Navigation Links
GenomiPhi amplification of FFPE tissue DNA for array CGH

tuting approximately 2600 human BAC clones at 1-Mb resolution. A section of hybridization images from the BAC arrays is shown in Figure 4. The signal intensities from array features were quantitated to compare hybridization performance. The scatter plots of mean intensities from DOP-PCR and GenomiPhi amplifications showed hybridization characteristics to be similar between the two methods (Fig 5). Microarray hybridization data for mean intensities and background was compared between GenomiPhi and DOP-PCR to assess amplification performance between the two techniques. The results are summarized in Table 2.



amplifications. Right: Hybridization image from GenomiPhi amplifications. DOP-PCR and GenomiPhi amplifications showed hybridization characteristics to be similar between the two methods (Fig 5). Microarray hybridization data for mean intensities and background was compared between GenomiPhi and DOP-PCR to assess amplification performance between the two techniques. The results are summarized in Table 2.

The total signal from the arrays (5200 features, in duplicate) was similar between the two amplification methods. With GenomiPhi amplification products, nearly equivalent signal was seen from both the Cy3 and Cy5 dyes, indicating that DNA amplified with the Phi29 enzyme can be efficiently labeled and hybridized with both dyes. The mean signal intensities from DOP-PCR and GenomiPhi amplified samples were similar, with values averaged from both the Cy3 and Cy5 dyes of 315 and 381, respectively. The averaged signal-to-background ratios from DOP-PCR and GenomiPhi amplified DNA were 4.92 and 5.02, respectively. The BAC array hybridization results demonstrate that FFPE samples can be amplified with the GenomiPhi kit for array CGH profiling. Hybridization data from the low-density Constitutional Chip™ 1.0 BAC array (Spectral Genomics) w
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10

Related biology technology :

1. Oragene and Whole Genome Amplification with GenomiPhi
2. GenomiPhi: protocol for BAC amplification and fabrication of slides for array CGH
3. Automation of TempliPhi and GenomiPhi DNA Amplification Kits on the Genesis Freedom Robotic Sample Processor
4. The TripleMaster PCR System for three different amplifications
5. Successful PCR amplification and subcloning of a GC-rich DNA fragment
6. Advantages of Roche Applied Science amplification products
7. Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.
8. Protocol for GenomePlex Whole Genome Amplification from Formalin-Fixed Parrafin-Embedded (FFPE) tissue
9. 2D-LC analysis of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
10. Sensitive identification of phosphopeptides in brain tissue using Ettan MDLC and Finnigan LTQ
11. High sensitivity quantitation of metabolites of nitrofuran antibiotics in animal tissue using LC/MS/MS
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: GenomiPhi amplification FFPE tissue DNA for array CGH

(Date:7/10/2014)... Francisco, CA (PRWEB) July 10, 2014 ... family closer together. The Archer Family purchased a ... contained within DNA, but ended up with a lifetime ... discoveries contained within the family members’ DNA genuinely brought ... had decided to search deeper into genetic history and ...
(Date:7/10/2014)... July 10, 2014 EvoDerma ’s NOOME ... This rejuvenating device now comes with a second treatment cup ... time. , The new cup is thinner on the edges ... to target rough, thin and uneven surfaces on the face, ... for a stimulating treatment on areas such as the cheeks ...
(Date:7/10/2014)... 2014 On July 9 Prime ... presented Russia,s first national "Industry" ... Company is developing a unique project called MabNext ... a number of innovative drugs based on monoclonal antibodies ... took place at the International Exhibition "Innoprom 2014" in ...
(Date:7/10/2014)... , July 10, 2014 Research ... addition of the "International Photonic Integrated Circuit ... Forecasts to 2019" report to their offering. ... The concept of photonic integration traces its ... century. The promise of photonic integration went unexplored ...
Breaking Biology Technology:Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 2Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 3Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 4Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 5Introducing a New Bonus Treatment Cup for EvoDerma’s NOOME Anti-Aging Motion 2Dmitry Medvedev Presented BIOCAD the First National "Industry" Award 2International Photonic Integrated Circuit (Monolithic Integration, Hybrid Integration, Module Integration) Market - Forecasts to 2019 2
... 2008, SAN DIEGO, May 12 Neurocrine Biosciences,Inc. ... will,present at the Citi Investment Research Global Health Care ... place Thursday, May 22,2008 at 3:00 p.m. Eastern Time ... simultaneously webcast and may be accessed on the,Company,s website ...
... MedApps announced today, that,it has been selected as ... as,part of Dell,s 2008 Global Small Business Excellence ... Health Monitoring System helps connect,people afflicted with chronic ... COPD, with their healthcare providers. This is,accomplished by ...
... Care, ST. LOUIS, May 12 Veran ... $4.75M in venture capital and private,financing to drive ... biopsy and ablation of cancer. St. Louis-based Prolog ... Capital Partners and existing private,investors. Veran also relocated ...
Cached Biology Technology:MedApps Selected as Dell-NFIB 2008 Business Excellence Award Finalist 2MedApps Selected as Dell-NFIB 2008 Business Excellence Award Finalist 3Veran Medical Technologies Secures $4.75M in First Institutional Funding 2
(Date:7/11/2014)... Research and Markets has announced ... - Industry Analysis Size Share Growth Trends and Forecast ... http://photos.prnewswire.com/prnh/20130307/600769 The rising ... need for high level security in both private and ... as username and passwords, tokens etc. are vulnerable to ...
(Date:7/10/2014)... Fe, Albuquerque, and other major cities in New Mexico, ... treated municipal wastewater rather than precious potable water supplies. ... 40% of all golf courses receive treated effluent. Reusing ... , Additionally, golf courses and homeowners alike fertilize their ... fertilizer is nitrate. A New Mexico State University turfgrass ...
(Date:7/10/2014)... , June 30, 2014  Aware, Inc. (NASDAQ: ... software and services, previously announced on June 26, 2014 ... cash dividend of $1.75 per share, or approximately $40 ... of July 10, 2014 and a payment date of ... NASDAQ that it had set an ex-dividend date for ...
Breaking Biology News(10 mins):International Biometrics Technology Market - Industry Analysis Size Share Growth Trends and Forecast to 2019 2'Tailored' water -- the latest in lawn care 2NASDAQ Sets Ex-Dividend Date for Aware, Inc.'s Special Cash Dividend of $1.75 Per Share 2
... and Ulm and Karlsruhe in Germany have shown that a ... a reef coral has excellent properties as a marker protein ... published online by Nature Methods and will appear ... Fluorescent proteins produced by a range of marine animals ...
... spiders, ability to manufacture silk that is light-weighted while ... finer than human hair, five times stronger by weight ... quality man-made fiber Kevlar, spider dragline silk is an ... ranged from parachute cords and protective clothing to composite ...
... support native bees that help maintain the health of ... to a study by a University of Michigan ... Berkeley. The study suggests that by pollinating native ... the bees help preserve the genetic diversity of remnant ...
Cached Biology News:Engineered coral pigment helps scientists to observe protein movement 2Engineered coral pigment helps scientists to observe protein movement 3Native-like spider silk produced in metabolically engineered bacterium 2Shade-coffee farms support native bees that maintain genetic diversity in tropical forests 2Shade-coffee farms support native bees that maintain genetic diversity in tropical forests 3
Cord blood endothelial progenitor cells, For immunohistochemistry (IHC) Cell Chip with Human Umbilical Cord Blood Progenitor Cells...
Umbilical cord blood CD14+ Monocytes, For immunohistochemistry (IHC) Cell Chip with Human Umbilical Cord Blood Monocytes, B Cells and NK Cells...
Human peripheral blood basophils, For immunohistochemistry (IHC) Cell Chip with Human Peripheral Blood Granulocytes...
Umbilical cord blood CD19+ B cells, For immunohistochemistry (IHC) Cell Chip with Human Umbilical Cord Blood Monocytes, B Cells and NK Cells...
Biology Products: