Navigation Links
Genetic Reporter Systems

Genetic reporter systems have developed into an essential tool for examining regulatory promoter and enhancer sequences as well as transcription factors. In most cases, the element under investigation (promoter, enhancer) is cloned together with the reporter gene in an expression vector, which is subsequently used to transfect cells. Quantification of the reporter indirectly provides information on the transcription activity of the element under investigation. Quantification can take place by detecting the corresponding RNA, the reporter protein, or by measuring the enzyme activity of the reporter protein. When the reporter system is selected, care must be taken to ensure that the reporter gene does not influence the physiology of the transfected cells and that the gene is not already endogenously expressed in the examined cells.

Reporter systems are also often used as a standard to compare the transfection efficiency of different transfection experiments. In this case, the control reporter system usually contains a constitutive promoter and a reporter gene, which is different to the reporter gene used by the element under examination. Such a reporter system can also be used to optimize electroporation parameters.

Chloramphenicol Acetyltransferase (CAT)

Chloramphenicol Acetyltransferase (CAT) This enzyme comes from microorganisms and catalyzes the transfer of acetyl groups from acetyl coenzyme A to chloramphenicol. With the CAT assay, the CAT-containing lysates of transfected cells are incubated with 14C-chloramphenicol, which is then acetylated. Acetylated and non-acetylated 14C-chloramphenicol is then separate
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Map and Link Human Genetic Disorders with SSLP Analysis
2. Genetic Reporter Systems
3. Purification and Characterization of beta-Lactoglobulin Genetic Variants A and B Using Preparative Electrophoresis and Isoelectric Focusing
4. Detection of Genetically Modified Soybean in Processed Foods Using Real-Time Quantitative PCR with SYBR Green I Dye on the DNA Engine Opticon 2 System
5. The iCycler iQ and Amplifluor Detection Systems for Analysis of Genetically Modified Organisms in Foodstuffs, Rev A
6. Analysis of Bacterial Assemblage Genetic Diversity in Environmental Samples Using the DCode System
7. Monitoring Bacterial Genetic Diversity in a Freshwater Lake Using TTGE and DNA Sequence Analysis
8. Rapid, High Throughput Mutation Discovery Using Fluorescent SSCP on the ABI PRISM 3100 and 3100Avant Genetic Analyzers
9. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
10. Stable HeLa Luciferase Reporter Cell Lines Expressing GAL4 Fusion Transactivators
11. Versatile Reporter Vectors for Monitoring Viral Transduction
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Genetic Reporter Systems

(Date:9/19/2014)... Florida , September 19, 2014 ... release latest developments, patents and results of studies and ... Eli Lilly and Company (NYSE: LLY ), ... Corporation (NASDAQ: CELG ) and Arena Pharmaceuticals ... a leader in developing prodrug therapeutics for the treatment ...
(Date:9/19/2014)... The new , Healthcare and Life Sciences Review ... PharmaBoardroom.com for free download , looks beyond ... economy in 2009, and the austerity measures that followed, to ... times of trouble. "Despite the crisis, ... and the general conditions to invest are favorable to the ...
(Date:9/18/2014)... A novel robotic system that can operate inside ... tested as part of a biomedical research partnership program ... aim of determining if the robot, in conjunction with ... more accurate, less costly, and less discomforting for the ... deliver prostate cancer therapies with greater precision. , Developed ...
(Date:9/18/2014)... Research and Markets has announced ... Cell Industry Report, 2014-2017" report to their offering. ... that can differentiate into specialized cells and can divide ... therapy can be applied to treatment of cardiovascular diseases, ... diseases, damage or lesion of liver, kidney and other ...
Breaking Biology Technology:Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 2Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 3Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 4Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 5Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 6Biotech Companies Pushing Sector Higher With Recent Therapeutic Advancements & Patent Approvals - Company Wins Summary Judgment For Prodrug Patent 7PharmaBoardroom Releases New Spain Pharmaceuticals Report 2PharmaBoardroom Releases New Spain Pharmaceuticals Report 3Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 2Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 3Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 4Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 5Worcester Polytechnic Institute Participates in Clinical Trial for MRI-Compatible Robot Designed to Improve Accuracy of Prostate Cancer Biopsies 6Global and Chinese Stem Cell Industry Report, 2014-2017 2Global and Chinese Stem Cell Industry Report, 2014-2017 3
... skin have been used to detect humans in a simulation ... for use in real-life situations, such as the devastating aftermath ... Zealand and Japan. Published today, Monday 12 September, in ... examined flumes of air to create a preliminary profile of ...
... A new system to be installed at ... exciting new research possibilities across the nation and ... and simulation, data science, high-performance computing, and web ... grant from the National Science Foundation (NSF) and ...
... 12, 2011 University Medical Center in Tucson (UMC) is ... assay (HER2 Dual ISH) on the BenchMark XT automated slide ... cancer patients in an effort to change the paradigm for ... Inc. (Ventana), a member of the Roche group, is the ...
Cached Biology Technology:Scientists utilize breath and sweat to detect trapped humans 2New supercomputer to boost Rensselaer leadership in high-performance computing 2New supercomputer to boost Rensselaer leadership in high-performance computing 3New supercomputer to boost Rensselaer leadership in high-performance computing 4Early Adopter of INFORM HER2 Dual ISH Assay Optimistic About Impact on Patient Care, Hospital Bottom Line 2Early Adopter of INFORM HER2 Dual ISH Assay Optimistic About Impact on Patient Care, Hospital Bottom Line 3Early Adopter of INFORM HER2 Dual ISH Assay Optimistic About Impact on Patient Care, Hospital Bottom Line 4
(Date:9/18/2014)... and ethnic groups describe losing eyesight as potentially ... more so than other conditions including: loss of ... 49% of non-Hispanic whites, 43% of Asians and ... ailment is the worst that could happen to ... AIDS/HIV. Hispanics and Asians ranked cancer first ...
(Date:9/18/2014)... rabbitfish which have devastated algal forests in the eastern ... Mediterranean basin if their distribution continues to expand as ... by an international team of researchers led by Dr ... of the Mediterranean Institute for Advanced Studies in Spain, ... Members of the team surveyed more than 1000 kilometres ...
(Date:9/18/2014)... when people are too stressed they are often grouchy, ... Mind Institute (BMI) at EPFL have just highlighted a ... stress and the loss of social skills and cognitive ... synaptic regulatory molecule in the brain. This was revealed ... , Carmen Sandi,s team went to look for ...
Breaking Biology News(10 mins):Americans rate losing eyesight as having greatest impact on their lives 2Americans rate losing eyesight as having greatest impact on their lives 3Tropical fish a threat to Mediterranean Sea ecosystems 2How stress tears us apart 2
... the second consecutive year, members of Virginia Tech,s ... in mechanical engineering, directed by professor Pavlos ... the Outstanding Paper Award in fluid mechanics from ... published by the American Institute of Physics. ...
... and Scott Ollinger of the University of New Hampshire,s ... (EOS) have received funding from the National Science Foundation ... for the agency,s new Macrosystems Biology program. The grant ... or so-called carbon "sources" and "sinks" in the long-term ...
... flat Prairie, you,d think a city would provide a good ... in trees and buildings. But researchers at the University of ... far from ideal for these animals. "I was really ... PhD graduate in biological sciences at the University of Calgary ...
Cached Biology News:Virginia Tech mechanical engineers win measurement science best paper award 2UNH researchers receive grant for pioneering carbon cycle model 2UNH researchers receive grant for pioneering carbon cycle model 3Big city holds empty promise for bats 2
... >95%NEN Radiolabeled Ligands\n\nReceptor-related research has long been ... radiolabeled ligands selected to keep pace with ... of products and services for receptor research ... radioligands. If you do not find exactly ...
... DNA polymerase is an antibody-mediated ... both conventional and real-time PCR ... at a concentration of 5 ... 5,000 units polymerase, 25 ml ...
... catalyzes the formation of a phosphodiester bonds ... nicked duplex DNA molecules or between oligonucleotides ... strand at 45 - 65?. Tfi DNA ... higher temperatures than conventional DNA ligases, Thus, ...
... contains a unique β-agarose digesting enzyme developed ... intact DNA and RNA from low melting ... TAE, TBE, MOPS, or phosphate buffers. The ... TAE, TBE, MOPS, and phosphate electrophoresis buffers ...
Biology Products: