( iency observed with supercoiled pUC18 D...)Generate High-Quality, Directional Plasmid cDNA Libraries

In circumstances where only limited amounts of starting material are available for constructing a cDNA library, the highest efficiencies are required. For these instances, it may be preferable to construct the library in a lambda vector, which offers 4-5 times more primary clones per microgram of RNA. Stratagenes unique Lambda ZAP vectors allow high-titer lambda libraries to be generated, and single lambda clones or entire Lambda ZAP libraries can be rapidly converted into a plasmid format.^6-10

Conclusions

Stratagenes new plasmid cDNA library construction kits are designed for generating high-quality, directional cDNA libraries in either the pBluescript II (prokaryotic), pCMV-Script (eukaryotic) or pAD-GAL4-2.1 (yeast two-hybrid) XR vectors. When screening cDNA libraries by functional methods, such as yeast two-hybrid screening, eukaryotic expression or antibody screening, directional cloning increases the probability of finding clones. These library construction kits are provided with Stratagenes cDNA Synthesis Kit as well as XL10-Gold ultracompetent cells for the highest transformation efficiency of ligated DNA.

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3. Gubler, U., and Hoffman, B.J. (1983) Gene 25: 263-269.

4. Costa, G.L., and Weiner, M.P. (1994) Nucleic Acids Res. 22: 2423.

5. Bullock, W.O., et al. (1987) Biotechniques 5: 376-379.

6. Short, J.M., et al. (1988) Nucleic Acids Res. 16: 7583-7600.

7. Alting-Mees, M., et al. (1992) Strategies 5: 58-61.

8. 
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