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Generate High-Quality, Directional Plasmid cDNA Libraries

Functional Screening

Mammalian cells

Protein-protein interactions
with bait protein in yeast

Special Applications

Sequencing for EST databases

Mammalian expression

Yeast two-hybrid screening

cDNA libraries are a central method for discovering new genes. Traditionally, genes have been selected for sequence homology; more recently, genes are being discovered by gene function. Both lambda and plasmid vectors have been used extensively to create cDNA libraries for these purposes. For functional screening applications, such as yeast two-hybrid screening, complementation studies and expressed sequence tag (EST) sequencing, cDNA libraries constructed directly into plasmid vectors are convenient and easy to use. However, constructing high-quality plasmid libraries can be limited by the efficiency of the cDNA synthesis reaction and the transformation of plasmid clones. Stratagene has improved the general methods for constructing plasmid cDNA libraries and offers three plasmid-based cDNA library construction kits using the pBluescript II (prokaryotic), pCMV-Script (eukaryotic) and pAD-GAL4-2.1 (yeast two-hybrid) vectors (table 1). In each system, directional cDNA synt
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