Navigation Links
GeneChip Sample Cleanup Module for cleanup of cDNA and in vitro ,,, transcribed cRNA

A crucial step in gene expression analysis is target labeling. To improve the critical steps in the target-labeling process, QIAGEN has developed the GeneChip Sample Cleanup Module specifically for use with Affymetrix GeneChip expression arrays. The GeneChip Sample Cleanup Module was developed as part of an ongoing collaboration between QIAGEN and Affymetrix, the market leader in microarray gene expression analysis. The GeneChip Sample Cleanup Module helps streamline and standardize preparation of target nucleic acids, to save time and effort in the lab and improve reproducibility (Figure 1).
Figure 1 Cleanup using the GeneChip Sample Cleanup Module was compared with a phenol/chloroform method previously used in GeneChip analyses. Brain, liver and HeLa total RNA (5 g) and yeast poly-A RNA (0.2 g) were used. A TaqMan -actin Control Reagents were used in real-time RT-PCR to quantify the amount of cDNA obtained before and after purification. Measurements were made in triplicate, and the mean value was used to calculate recovery. B After cleanup, 10 l of the 12 l of recovered cDNA was used for in vitro transcription. The cRNA yields were determined by UV spectrophotometry after adjustment by subtracting carryover RNA. Advantages of the GeneChip Sample Cleanup Module:

  • Fast, easy-to-use, and reliable spin-column procedure for cDNA and cRNA cleanup

  • Optimized elution volumes compatible with assay flow, eliminating the need to concentrate samples

  • Conveniently packaged to facilitate 3 steps in the target preparation procedure - cDNA c leanup, in vitro-transcribed cRNA cleanup, and cRNA fragmentation

  • Developed and manufactured by QIAGEN specifically for GeneChip arrays

  • Extensively tested on GeneChip arrays with many sample types, including brain, kidney, liver, prostate, skin, placenta, blood, white adipose, and various cell lines




'"/>

Source:


Page: All 1 2

Related biology technology :

1. New statistical algorithms for Monitoring Gene Expression on GeneChip® Probe Arrays
2. The Affymetrix GeneChip Scanner 3000 New Advances in Scanner Design for Superior Performance, Reliability, and Dynamic Range
3. The New Affymetrix GeneChip Scanner 3000
4. Sample Preparation for Microinjection
5. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
6. A Better Way to Isolate RNA AND Protein From the Same Sample
7. Isolate Total RNA and Protein From the Same Sample
8. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
9. Preserve Samples for RNA Expression Microarrays RNAlater Around the World
10. RNA from LCM Samples
11. RNA, DNA, and Protein from a Single Sample
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:5/18/2017)... ... May 18, 2017 , ... Lajollacooks4u ... Cooking Challenge is a two-hour team-building package designed for groups of 10-30 people. ... Jodi Abel, which include items, such as Blackened Shrimp with Edamame Salad, Pizza ...
(Date:5/18/2017)... (PRWEB) , ... May 17, 2017 , ... NDA Partners ... executive and former CEO of Eurofins Advantar Laboratories and President of Pharmaceutical Development Business ... to his position at Eurofins and Cardinal Health, he was former Chief Operating Officer ...
(Date:5/18/2017)... ... May 17, 2017 , ... Cognition Corporation ... commercialization, has just released version 9.0 of the Cognition Cockpit platform. , “Our ... Cockpit,” says David Cronin, CEO of Cognition. “We’re thrilled to finally be able ...
(Date:5/18/2017)... , ... May 17, 2017 , ... ... equipment for varying industries, including food and dairy, munitions, and pharmaceutical/biotech, recently introduced ... safety and ease of use. The improvement in technology comes on the heels ...
Breaking Biology Technology:
(Date:4/24/2017)... 2017 Janice Kephart , former ... Strategy Partners, LLP (IdSP) , today issues the ... Trump,s March 6, 2017 Executive Order: Protecting ... can be instilled with greater confidence, enabling the ... refugee applications are suspended by until at least ...
(Date:4/13/2017)... April 13, 2017 According to a new market ... Identity Analytics, Identity Administration, and Authorization), Service, Authentication Type, Deployment Mode, Vertical, ... Market is expected to grow from USD 14.30 Billion in 2017 to ... of 17.3%. ... MarketsandMarkets Logo ...
(Date:4/6/2017)... 2017 Forecasts by Product Type ... by End-Use (Transportation & Logistics, Government & Public Sector, ... Fossil Generation Facility, Nuclear Power), Industrial, Retail, Business Organisation ... Are you looking for a definitive report on the ... ...
Breaking Biology News(10 mins):