Navigation Links
GeneChip Sample Cleanup Module for cleanup of cDNA and in vitro ,,, transcribed cRNA

A crucial step in gene expression analysis is target labeling. To improve the critical steps in the target-labeling process, QIAGEN has developed the GeneChip Sample Cleanup Module specifically for use with Affymetrix GeneChip expression arrays. The GeneChip Sample Cleanup Module was developed as part of an ongoing collaboration between QIAGEN and Affymetrix, the market leader in microarray gene expression analysis. The GeneChip Sample Cleanup Module helps streamline and standardize preparation of target nucleic acids, to save time and effort in the lab and improve reproducibility (Figure 1).
Figure 1 Cleanup using the GeneChip Sample Cleanup Module was compared with a phenol/chloroform method previously used in GeneChip analyses. Brain, liver and HeLa total RNA (5 g) and yeast poly-A RNA (0.2 g) were used. A TaqMan -actin Control Reagents were used in real-time RT-PCR to quantify the amount of cDNA obtained before and after purification. Measurements were made in triplicate, and the mean value was used to calculate recovery. B After cleanup, 10 l of the 12 l of recovered cDNA was used for in vitro transcription. The cRNA yields were determined by UV spectrophotometry after adjustment by subtracting carryover RNA. Advantages of the GeneChip Sample Cleanup Module:

  • Fast, easy-to-use, and reliable spin-column procedure for cDNA and cRNA cleanup

  • Optimized elution volumes compatible with assay flow, eliminating the need to concentrate samples

  • Conveniently packaged to facilitate 3 steps in the target preparation procedure - cDNA c
    '"/>


Source:


Page: All 1 2

Related biology technology :

1. New statistical algorithms for Monitoring Gene Expression on GeneChip® Probe Arrays
2. The Affymetrix GeneChip Scanner 3000 New Advances in Scanner Design for Superior Performance, Reliability, and Dynamic Range
3. The New Affymetrix GeneChip Scanner 3000
4. Sample Preparation for Microinjection
5. Quantitation of the Protein MIA as a Marker for Chondrocytes in Research Samples
6. A Better Way to Isolate RNA AND Protein From the Same Sample
7. Isolate Total RNA and Protein From the Same Sample
8. Isolate High Quality Total RNA from LCM Samples Suitable for Microarray and qRT-PCR Analysis
9. Preserve Samples for RNA Expression Microarrays RNAlater Around the World
10. RNA from LCM Samples
11. RNA, DNA, and Protein from a Single Sample
Post Your Comments:
(Date:7/2/2015)... , July 2, 2015 Amici ... Bio-tech procurement company Amici Procurement has chosen Microsoft Cloud ... it move to Office 365.      (Logo: ... small to medium-sized companies in the bio-tech sector with ... of the purchasing cycle to help them obtain best ...
(Date:7/2/2015)... (PRWEB) , ... July 02, 2015 , ... ... development and manufacturing of surgical instruments in Orthopedics, announced today the acquisition of ... and the world's largest provider of surgical instruments to the Spine Industry. The ...
(Date:7/1/2015)... PARIS , July 1, 2015 ... key developer of biotech crops, today announce a ... generations of insect control traits. "Growers ... the realities of advancing insect resistance. We are ... insect control technology with Genective,s capability in developing ...
(Date:7/1/2015)... ... July 01, 2015 , ... The next-generation sequencing (NGS) ... market segments, including the molecular diagnostics industry. BCC Research reveals in its new report ... tests, lower test costs, and a growing need for better diagnostics as part of ...
Breaking Biology Technology:Biotech Procurement Company Moves to Office 365 with iomart 2In'Tech Medical Acquires Turner Medical to Become a World Leader in Orthopedic Contract-Manufacturing 2In'Tech Medical Acquires Turner Medical to Become a World Leader in Orthopedic Contract-Manufacturing 3AgBiome and Genective collaborate to create novel insect-resistant crops 2NGS-Based Diagnostics, the ‘Must-Have Platform’, Sends Market Segment CAGRs Soaring According to BCC Research 2NGS-Based Diagnostics, the ‘Must-Have Platform’, Sends Market Segment CAGRs Soaring According to BCC Research 3
... September 10 Elsevier,a leading publisher of scientific, technical ... become one of the Charter Members of the,InChI Trust ... the first InChI Trust Board,meeting on September 11th. , ... Union of Pure and Applied,Chemistry (IUPAC), the IUPAC International ...
... , ROTTERDAM, The Netherlands, September 10 ... development of human,antibodies using its proprietary SIMPLE Antibody(TM) platform, today,announced ... The Company raised EUR 9.5million ( USD 13.6 million) from ... one of,the largest of its kind in the last 12 ...
... James Tour have brought graphite,s potential as a mass ... created the potential for reprogrammable gate arrays that could ... In a paper published in the online ... Alexander Sinitskii show how they,ve used industry-standard lithographic techniques ...
Cached Biology Technology:Elsevier Joins InChI Trust as a Charter Member 2Elsevier Joins InChI Trust as a Charter Member 3Elsevier Joins InChI Trust as a Charter Member 4arGEN-X Raises EUR9.5 Million to put SIMPLE Antibody(TM) Platform to Work 2arGEN-X Raises EUR9.5 Million to put SIMPLE Antibody(TM) Platform to Work 3arGEN-X Raises EUR9.5 Million to put SIMPLE Antibody(TM) Platform to Work 4arGEN-X Raises EUR9.5 Million to put SIMPLE Antibody(TM) Platform to Work 5Graphitic memory techniques advance at Rice 2Graphitic memory techniques advance at Rice 3
(Date:6/17/2015)... , and HILDEN, Germany , June ... QGEN ; Frankfurt Prime Standard: QIA) today launched new Investigator ... in forensic laboratories in the United States ... to simultaneously analyze multiple key genomic markers (short tandem repeats ... Quality Sensor to evaluate the quality of DNA in ...
(Date:6/16/2015)... Fingerprint Cards has received an order ... company,s portfolio from one of its module partners in ... place during the third quarter 2015. The sensors will be ... Jörgen Lantto, CEO of FPC, comments: " This ... interest from smartphone OEMs in integrating touch fingerprint sensors in ...
(Date:6/12/2015)... DALLAS , June 12, 2015  Securus ... justice technology solutions for public safety, investigation, corrections ... issued its patent to electronically capture, analyze and ... various facial recognition methods. This system ... authorized by an enrollment and subsequent sign-on process ...
Breaking Biology News(10 mins):QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 2QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 3QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 4QIAGEN Launches New Genetic Fingerprinting Kits for U.S. Forensic Labs 5FPC Receives Order for Touch Fingerprint Sensors of 375 MSEK 2Securus Announces Patent Issued for Authorized Presence Verification During Video Visitation 2
... mice responsible for repairing DNA strands damaged by oxidation ... that such stress contributes to the development of cancer. ... DNA Repair by researchers at the National Institute ... University (OHSU) and the New York University School of ...
... measure organic contaminants in human body fluids, the National ... four new Standard Reference Materials (SRMs) available for purchase. ... and Prevention (CDC), the human milk and serum SRMs ... pesticides, commonly found in the U.S. population. Scientists at ...
... Netherlands: For the first time, researchers have been able ... of IVF treatment in blood. Dr. Cathy Allen, from the ... the European Society of Human Reproduction and Embryology today (Wednesday ... works for some patients but not for others. Previous ...
Cached Biology News:Genetically engineered mice yield clues to 'knocking out' cancer 2NIST issues human milk and blood serum SRMs for contaminant measurements 2Will IVF work for a particular patient? The answer may be found in her blood 2Will IVF work for a particular patient? The answer may be found in her blood 3
Non-Protein Peroxidase Stabilizer , 1 L...
... the cellular components necessary for protein synthesis ... termination factors) but has not been treated ... primarily for the isolation of these components ... globin mRNA. Untreated Lysate is prepared from ...
... All MagCellect Ferrofluid products ... conjunction with R&D Systems MagCellect ... capacities, this neodymium iron boron ... 12 x 75 mm test ...
... converts Cap 0 (or Cap 0-like) RNA ... structure has been shown to increase in ... This improvement is seen in Cap 0 ... System as well as those Cap 0 ...
Biology Products: