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Gene-Specific Primers for NMDA Receptor Subunits

en seen in recombinant systems with heterologous coexpression of various NR2 subunits and/or NR1 splice variants.5 However, little is known about the expression of the NMDAR in situ.

NMDA Receptor Subunit

Expected PCR Product Size (bp)

NR1, Rat; flanking C1 exon

C1+, 214 C1, 103

NR1, Rat: flanking N1 exon

N1+, 235 N1,172

NR2A, Rat

207

NR2B, Rat

183

NR1, Human

782

Table 1

figure 1

RT-PCR assays with gene-specific primer sets can be used to study gene expression of the NMDA receptor subunits.6 Stratagenes cDNA library templates and amplification primer sets6 for rat NMDA subuni
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