Navigation Links
Gene-Expression Analysis of TP, DPD, and TS Using,,,the LightCycler mRNA Quantification KitsPLUS

In human cells, the enzymes thymidin phosphorylase (TP), dihydropyrimidine dehydrogenase (DPD), and thymidylate synthase (TS) play a pivotal role in the metabolism of pyrimidines. In cancer disease medical care, the cytotoxic compound 5-fluorouracil (5-FU) is the standard chemotherapy treatment for a wide range of solid tumors. Attempts to increase the efficacy and tolerability of fluoropyrimidine treatment has led to the development of Capecitabine (Xeloda), a fluoropyrimidine drug from Roche Pharmaceuticals. Capecitabine is not cytotoxic unless it is activated to 5-FU by TP a three-step mechanism. Therefore, it has several advantages over ordinary 5-FU by treatment [1, 2].



Capecitabine is preferentially activated and converted to 5-FU due to TP upregulation at the tumor site. 5-FU inhibits TS, an enzyme crucial for the de novo synthesis of thymidine nucleotides. The rate-limiting enzyme in the catabolism of 5-FU is DPD, which catalyzes the first step of pyrimidine degradation (Figure 1).

Efficacy and tolerability of Capecitabine or similar drugs may therefore largely depend on the expression rates of each of the three enzymes. These expression rates can be estimated by the detection of the relative mRNA expression levels of the three enzymes in tumor tissue or blood [3], using the new LightCycler TP, DPD, and TS mRNA Quantification KitsPLUS.

Materials and Methods

Fresh-frozen tissue and paraffin-embedded tissue (PET) slides (5 m) were taken from human xenografts in mouse models. Isolation of RNA was performed using the MagNA Lyser Instrument and the High Pure RNA Paraffin Kit. The human RNA
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. The Flexible matriXarray Chip System: A New Perspective for Probe Optimization in Gene-Expression Profiling
2. A Positive Selection Assay for Mutation Analysis in Big Blue Animals
3. Epitope-Tagging Vectors for Functional Analysis in Yeast
4. Map and Link Human Genetic Disorders with SSLP Analysis
5. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
6. Single-cell RT-PCR Analysis of Paramecium Primaurelia with the Eppendorf cMaster RTplusPCR System
7. Structural Analysis of Glycosylated Peptides in Complex Mixtures with Ion Trap MSn
8. Use of Transcriptor Reverse Transcriptase in Microarray Analysis
9. Increased Sensitivity in Microarray Analysis
10. Agilent 2100 Bioanalyzer Automated Analysis System
11. Analysis of Microarray Data
Post Your Comments:
(Date:8/27/2014)... 2014 In 2013, Lawrence ... Livermore National Security LLC (LLNS), was awarded ... a state-of-the-art laser system for the European Union's ... construction in the Czech Republic. , When commissioned ... called the " High repetition-rate Advanced Petawatt Laser ...
(Date:8/27/2014)... survive impact by the huge amounts of energy ... energy for photosynthesis. The hypothesis is that the ... the energy throughout the entire protein molecule through ... now managed to successfully ,film, this process. , ... which play a role in photosynthesis and thus ...
(Date:8/27/2014)... , Aug. 27, 2014 Research ... "Global Lactic Acid  (Biodegradable Polymer, Food & ... & Forecasts to 2019" report to their ... hydroxyl-carboxylic acid with an asymmetrical carbon atom. The ... biodegradable polymer, food and beverage, personal care product, ...
(Date:8/27/2014)... “We are honored to be named ... Thangaraj, CEO of Radiant Sage. “We are offering truly ... imaging corelab market to a different level. We ... to efficiently and effectively manage the massive amounts of ... analyzed and read by trained physicians. Being recognized by ...
Breaking Biology Technology:LLNL Synchs up with ELI Beamlines on Timing System 2LLNL Synchs up with ELI Beamlines on Timing System 3DTU researchers film protein quake for the first time 2Global Lactic Acid (Biodegradable Polymer, Food & Beverage, Personal Care & Pharmaceutical) Market - Trends & Forecasts to 2019 2Radiant Sage Named to CIOReview List of 100 Most Promising Technology Companies 2
... Host Conference Call Today at 5:00 p.m., CAMBRIDGE, ... announced today that the FDA has informed the,company of ... bioreactor scale and Myozyme produced at the 2000L scale ... in the,carbohydrate structures of the molecules. Currently, Genzyme has ...
... at DiscoverTesting.com, Test Kit Developed in Response to ... Consumer Demand for ... Research unveils,its newest Watersafe product this week, addressing consumer concerns,regarding ... Test Kit tests for ten contaminants with easy-to-use,at-home results, most ...
... ATLANTA, April 21 Biofisica Inc., an Atlanta-based,company ... it has,obtained a commitment of $2.3 million in ... marketing efforts in the UK and,ongoing planning initiatives ... ), The round was led by Unilever ...
Cached Biology Technology:Genzyme Provides Update on Myozyme(R) Manufacturing 2Genzyme Provides Update on Myozyme(R) Manufacturing 3Genzyme Provides Update on Myozyme(R) Manufacturing 4Genzyme Provides Update on Myozyme(R) Manufacturing 5Silver Lake Research Unveils Watersafe(R) Well Water Test Kit 2Biofisica Obtains $2.3 Million in Financing From Investors 2
(Date:8/27/2014)... a single fungal cell or bacterial colony exerts on a ... heavy role in setting up some of the most fundamental ... too much of a stretch to say that plants may ... to the touch of beneficial fungi, according to a new ... the University of Wisconsin-Madison. , "Many people have studied ...
(Date:8/27/2014)... -- WaferGen Bio-systems, Inc. (NASDAQ: WGBS) today announced that it ... units of common stock and warrants at a price ... million, prior to deducting underwriting discounts and commissions and ... warrants are immediately separable and will be issued separately.  ... consists of 2,000 shares of common stock and 2,000 ...
(Date:8/27/2014)... mastodons, short-faced bears, giant ground sloths, saber-toothed cats ... 13,000 years ago at the end of the Pleistocene ... been debated by scientists who, until recently, could only ... including UC Santa Barbara,s James Kennett, professor emeritus in ... collision with Earth played a major role in the ...
Breaking Biology News(10 mins):A touching story: The ancient conversation between plants, fungi and bacteria 2A touching story: The ancient conversation between plants, fungi and bacteria 3WaferGen Bio-Systems Announces Successful Completion of Public Offering Raising $20 Million 2WaferGen Bio-Systems Announces Successful Completion of Public Offering Raising $20 Million 3Nanodiamonds are forever 2Nanodiamonds are forever 3
... that shifts the body's fuel consumption from glucose to fat ... team led by scientists at The University of Texas Medical ... Nature. , While their findings could provide new ... signal that emerged from the dark also presents a new ...
... newly identified human bocavirus was found in nearly 5 ... very young children. Researchers from the Centers for ... the International Conference on Emerging Infectious Diseases , "Our ... with pneumonia in Thailand, especially among young children," says ...
... Institute of Allergy and Infectious Diseases (NIAID), part of ... defect in the immune response of people with the ... of developing serious complications following smallpox vaccination. Led by ... Medical and Research Center in Denver, the researchers used ...
Cached Biology News:Darkness unveils vital metabolic fuel switch between sugar and fat 2Darkness unveils vital metabolic fuel switch between sugar and fat 3Bocavirus infection may be associated with pneumonia in Thailand, especially in children 2Defective immune system response to smallpox vaccine detailed in new study 2Defective immune system response to smallpox vaccine detailed in new study 3
... fast emerging as a powerful new tool ... now, only amino groups have been able ... such as our Sulfo-SBED. Sulfhydryl-reactive Mts-Atf-Biotin and ... additions to the protein interaction tool chest ...
...
... compact benchtop and stackable multi-function incubator. ... a large display screen to accurately ... circulation inside to evenly distribute temperature ... easily calibrated from the microprocessor controller. ...
...
Biology Products: