Navigation Links
GENE SILENCING WITH PCR PRODUCTS

east two different promoters and transfect them into the cells being targeted for gene knockdown (Figures 3, 4, 6, and 7). The promoter that is more effective for the siRNA and cell type will provide greater levels of gene silencing.


SEC Validation
The Silencer Express Kits have been used to create SECs targeting to GAPDH, c-fos, cyclophilin, PKC-alpha, -actin, and Green Fluorescent Protein (GFP). In all cases, SECs were able to reduce target gene expression. Figures 14 and 6 show several examples. Furthermore, SECs targeting GAPDH were functional in HeLa, 293, Cos7, Du145, HepG2, NIH3T3, MCF7 and A549 cells, confirming that PCR products can be transfected and transcribed in different cell types. Because they are simple to prepare, SECs provide an ideal way to screen potential siRNA sequences for the best siRNA sequence-promoter combination for DNA-based RNAi experiments in diverse cell lines. For additional information and data, click here.

Figure 6. Effectiveness of RNA Pol III Promoters with Altered 3' Ends. siRNA Expression Cassettes featuring the human U6 (Hu-U6) and human H1 (Hu-H1) promoters and expressing Negative Control (scrambled), GAPDH-, and cyclophilin-specific hairpin siRNAs were transfected into 293 cells. 72 hours post-transfection, the cells were processed to recover total RNA. Approximately 500 ng of total RNA from each sample was assessed by Northern analysis. Radiolabeled RNA probes targeting 28S rRNA, GAPDH, and cyclophilin were used to detect target genes and normalize levels of input RNA. Films were exposed to t
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: GENE SILENCING WITH PCR PRODUCTS

(Date:12/15/2014)... 2014  Origin Agritech Limited (NASDAQ GS: SEED) (" Origin ... crop seeds in China , today announced ... 2014 ended September 30, 2014, before the market opens on ... a teleconference on January 8, 2015, at 8:00 a.m. ET ... discuss the results. To participate in the call, please dial ...
(Date:12/13/2014)... The DNA Microarray market market was ... expected to grow at a CAGR of 15.2% ... TOC of the DNA Microarray for an in-depth ... help of various tables and figures. , ... consists of instrument, consumables, and services, where the ...
(Date:12/13/2014)... 2014  Vermillion, Inc. (NasdaqCM: VRML), a bio-analytical solutions company ... Veronica G. H. Jordan , Ph.D., and ... Directors, bringing the total number of directors to eight, ... Jim LaFrance , Chairman, President, and Chief Executive Officer ... the appointments of Veronica and David to our board ...
(Date:12/12/2014)... Graphite Metallizing Corporation, the manufacturer of self-lubricating ... has appointed Kirk Staller as Sales Engineering Representative for ... a B.S. degree from Purdue University and has over ... of it with a manufacturer of centrifugal pumps. He ... , “We are pleased to have Kirk join ...
Breaking Biology Technology:Origin Agritech Limited to Announce Fiscal 2014 Annual Financial Results on January 8th, 2015 2Origin Agritech Limited to Announce Fiscal 2014 Annual Financial Results on January 8th, 2015 3DNA Microarray Market Is Expected to Grow at a CAGR of 15.2% from 2014 to 2019 - A Report by MicroMarket Monitor 2DNA Microarray Market Is Expected to Grow at a CAGR of 15.2% from 2014 to 2019 - A Report by MicroMarket Monitor 3DNA Microarray Market Is Expected to Grow at a CAGR of 15.2% from 2014 to 2019 - A Report by MicroMarket Monitor 4Vermillion Strengthens Board of Directors with Appointments of Veronica Jordan, Ph.D., and David Schreiber 2Vermillion Strengthens Board of Directors with Appointments of Veronica Jordan, Ph.D., and David Schreiber 3Kirk Staller Joins Graphite Metallizing Corporation 2
... WA, and VANCOUVER, Oct. 9 /PRNewswire-FirstCall/ - OncoGenex Pharmaceuticals, ... Cormack, president and chief executive officer of OncoGenex, has ... The Year(R). The Ernst & Young Entrepreneur Of The ... entrepreneurs that are building successful, dynamic businesses. , "I ...
... ... Supersaturation Assessment and Control for the Development and Optimization of Crystallization Processes, a method ... for the production and control of qualitative supersaturation trajectories. , ... (PRWEB) October 9, 2009 -- The quantitative ...
... , ... is announcing its 2010 Best Practices awards program and is opening the call for ... of technology and strategic innovation--initiatives and collaborations that manifestly improve some facet of the ... ...
Cached Biology Technology:OncoGenex Pharmaceuticals CEO Named Canada's Pacific Ernst & Young's Entrepreneur Of The Year(R) in the Health Sciences Category 2OncoGenex Pharmaceuticals CEO Named Canada's Pacific Ernst & Young's Entrepreneur Of The Year(R) in the Health Sciences Category 3Calibration Free Supersaturation Assessment and Control for the Development and Optimization of Crystallization Processes 2The 2010 Bio-IT World Best Practices Call for Entries has been Released -- Highlight your Company's Excellence in Innovation 2The 2010 Bio-IT World Best Practices Call for Entries has been Released -- Highlight your Company's Excellence in Innovation 3
(Date:12/10/2014)... Research and Markets ( http://www.researchandmarkets.com/research/xclcvw/biometrics_market ... "Biometrics Market in Japan 2014-2018" report to ... The integration of biometrics and ... and upgradation of the driver,s license is one ... Besides the aforementioned projects, biometrics is being used ...
(Date:12/4/2014)... , Dec. 3, 2014  Crossmatch™, a ... announced that its DigitalPersona ® Pro Enterprise ... have been deployed throughout First Bank branch locations ... South Carolina and Virginia. First Bank, ... Southern Pines, North Carolina , selected ...
(Date:11/21/2014)... 2014  Earlier this year Donald Spector , ... one of the most prolific inventors in the world, ... from Smartphones to third party agencies. Spector envisioned this ... of the earliest known patents in this area. Now ... military, child care, elder care and hospital applications. This ...
Breaking Biology News(10 mins):Biometrics Market in Japan 2014-2018: Key Vendors are DDS, Fujitsu, Hitachi and NEC 2First Bank Partners with Crossmatch to Increase Log In Security 2First Bank Partners with Crossmatch to Increase Log In Security 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 2Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 3Patented Biosensor Wearable Technology Provides a More Civilized Method of Quarantine 4
... Two scientists from Cold Spring Harbor Laboratory (CSHL) are ... genetic mutation that causes Joubert Syndrome. JBTS, as it ... that is very rare in the general population but ... The study was published in collaboration with Dor Yeshorim, ...
... crops relies on a relatively stable climate, not dramatic ... evolutionary, ecological, genetic and agronomic considerations, Dr. Shahal Abbo, ... Hebrew University of Jerusalem, Israel, and colleagues, demonstrate why ... domestication in the Near East. Rather, the variety of ...
... COLUMBIA, Md. , Jan. 12 Cylex Incorporated® ... Panel approved the creation of new CPT coding based on ... the fact that existing CPT codes insufficiently captured all steps ... , In an effort to keep customers informed of these ...
Cached Biology News:CSHL scientists use next-gen sequencing to rapidly discover genetic cause of devastating disorder 2Stable climate and plant domestication linked 2Cylex(TM) Awarded New CPT Code For Its ImmuKnow(R) Assay 2Cylex(TM) Awarded New CPT Code For Its ImmuKnow(R) Assay 3
... is the simplest, easiest-to-use system ever for ... handling capabilities,into their labs, or groups in ... applications on a separate instrument. Plate-handling is ... even when you have thousands of samples., ...
Cells are grown in triple flasks, roller bottles, or NUNC Factory. Viability and growth curves are generated from a parallel representative culture....
NovaScreen offers cell biology, cell culture and molecular biology services for companies who lack a cell culture facility....
... Affinity Life Sciences provides cost effective cell ... proteins or monoclonal antibodies. Quality at a ... whether you require research or commercial product, ... production methods such as roller bottles, hollow ...
Biology Products: