Advantages of siRNA Expression Cassettes
In contrast to siRNA expression vectors, which require cloning and sequencing prior to use and can take 1-2 weeks to prepare, SECs can be prepared in less than a day. SECs thus provide an excellent screening tool to find the most effective siRNA sequence, or to identify the best combination of promoter and siRNA sequence in the experimental system being used. In fact, SECs provide the perfect complement to siRNA expression vectors. By incorporating restriction sites at their ends, SECs found to effectively elicit gene silencing can be readily cloned into a plasmid to create an siRNA expression vector. The siRNA expression vector can then be used for stable expression and long term studies.
Silencer Express Kits
Ambion's Silencer Express Kits provide the reagents necessary to prepare SECs with the Human H1, Human U6, or Mouse U6 promoters. Each kit includes a DNA fragment containing one of the