Navigation Links
GENE SILENCING WITH PCR PRODUCTS

e U6 (Mo-U6), human U6 (Hu-U6), and human H1 (Hu-H1) promoters and encoding a -actin-specific hairpin siRNA were transfected into HeLa cells. 72 hours post-transfection, the cells were assessed using nuclear staining with DAPI and immunofluorescence using a -actin antibody. Non-transfected cells (NT) as well as cells transfected with an SEC expressing a negative control siRNA (scramble) provide wild-type levels of -actin. The relative level of reduction in gene expression was quantified and is provided in the bar graph.


Advantages of siRNA Expression Cassettes
In contrast to siRNA expression vectors, which require cloning and sequencing prior to use and can take 1-2 weeks to prepare, SECs can be prepared in less than a day. SECs thus provide an excellent screening tool to find the most effective siRNA sequence, or to identify the best combination of promoter and siRNA sequence in the experimental system being used. In fact, SECs provide the perfect complement to siRNA expression vectors. By incorporating restriction sites at their ends, SECs found to effectively elicit gene silencing can be readily cloned into a plasmid to create an siRNA expression vector. The siRNA expression vector can then be used for stable expression and long term studies.


Silencer Express Kits
Ambion's Silencer Express Kits provide the reagents necessary to prepare SECs with the Human H1, Human U6, or Mouse U6 promoters. Each kit includes a DNA fragment containing one of the
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8

Related biology technology :

1. IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: GENE SILENCING WITH PCR PRODUCTS

(Date:7/29/2014)... 2014 Human Longevity, Inc. (HLI), a genomics ... extending the healthy, high performance human life span, today ... an expert in machine learning and machine translation as ... from Google where he was Distinguished Research Scientist and ... to HLI Co-Founder and CEO, J. Craig Venter ...
(Date:7/29/2014)... ELLICOTT CITY, Md. , July 29, 2014 ... has announced a new and updated program for ... October 10 – 12, 2014 in Amelia ... sponsors, CROs, sites and investigators remains unchanged, however, ... more purposeful exchange and networking opportunity among all ...
(Date:7/29/2014)...  Discovery Laboratories, Inc. (Nasdaq: DSCO),  a specialty ... of respiratory critical care, will report its second quarter ... the open of the U.S. financial markets.  ... teleconference and webcast at 9:00 a.m. Eastern Time that ... management will discuss the 2014 second quarter financial ...
(Date:7/29/2014)... July 29, 2014  ImmunoClin Corporation (IMCL) is ... treatment of infectious diseases as well as nutraceuticals. ... prevention of pathologies like cardiovascular disease, Alzheimer,s disease ... ImmunoClin Corporation will complete the strategic relocation of ... , a key center of North American ...
Breaking Biology Technology:Franz Och, Ph.D., Expert in Machine Learning and Machine Translation, Joins Human Longevity, Inc. as Chief Data Scientist 2Franz Och, Ph.D., Expert in Machine Learning and Machine Translation, Joins Human Longevity, Inc. as Chief Data Scientist 3TransCelerate, Lilly, Pfizer, and FDA Highlight Collaborative Program at the Site Solutions Summit 2TransCelerate, Lilly, Pfizer, and FDA Highlight Collaborative Program at the Site Solutions Summit 3Discovery Labs to Report 2014 Second Quarter Financial Results and Hold Conference Call on Tuesday, August 5, 2014 at 9:00 a.m. Eastern Time 2ImmunoClin Corporation (IMCL) Relocates Corporate Headquarters to Washington, DC 2ImmunoClin Corporation (IMCL) Relocates Corporate Headquarters to Washington, DC 3
... , MONTREAL, Dec. 7 /PRNewswire/ - Enerkem Inc. ... been selected by the U.S. Department of Energy (DOE) to ... of its waste-to-biofuels facility to be located in Pontotoc, Mississippi. ... been selected in the larger demonstration scale project category of ...
... 7 ATS Medical, Inc. (Nasdaq: ATSI ), ... services today announced it has received CE Mark approval ... the European Union, indicating it is compliant with relevant ... The Enable valve is the first surgical aortic valve ...
... ... develop systems biology-driven therapeutics to include small molecules, chemotherapy and RNAi candidates and ... ... announced today that it has acquired Hermes Biosciences, Inc. a private biotechnology company ...
Cached Biology Technology:Enerkem Awarded $50 Million Funding by U.S. Department of Energy for its Mississippi Biorefinery Project 2Enerkem Awarded $50 Million Funding by U.S. Department of Energy for its Mississippi Biorefinery Project 3ATS Medical Announces CE Mark Approval for the ATS 3f Enable Bioprosthesis 2ATS Medical Announces CE Mark Approval for the ATS 3f Enable Bioprosthesis 3ATS Medical Announces CE Mark Approval for the ATS 3f Enable Bioprosthesis 4Merrimack Pharmaceuticals Acquires Hermes Biosciences, Inc. 2Merrimack Pharmaceuticals Acquires Hermes Biosciences, Inc. 3
(Date:7/29/2014)... new study from UC San Francisco is the first ... accumulate overtime and accelerate cellular aging, these negative effects ... and sleeping well. , "The study participants who ... shortening than the ones who didn,t maintain healthy lifestyles, ... lead author Eli Puterman, PhD, assistant professor in the ...
(Date:7/28/2014)... the United States have a circulatory problem of the ... and may even require surgery in serious cases. This ... in turn, limb amputation. , At The University of ... scientists tested a non-surgical preventative treatment in a mouse ... increased blood circulation. Their proof-of-concept study appears in ...
(Date:7/28/2014)... a wild relative of the domestic tomato, has been ... labs headed by Professors Neelima Sinha and Julin Maloof ... new genome information may help breeders produce tastier, more ... the journal Nature Genetics , was lead by ... The UC Davis labs carried out work on the ...
Breaking Biology News(10 mins):Healthy lifestyle may buffer against stress-related cell aging, study says 2Scientists discover genetic switch that can prevent peripheral vascular disease in mice 2
... Naturforschung will collaborate on the publication of two ... Palaeobiodiversity and Palaeoenvironments and Marine Biodiversity ... through Springer,s abstracting and indexing services and innovative ... both electronic and print versions. Palaeobiodiversity ...
... Current research suggests that a new technique to ... tissue samples. The related report by Balic et ... tissues from colorectal cancer patients using quantitative high-resolution melting ... Journal of Molecular Diagnostics . DNA in ...
... genome eight years ago, enormous progress has been made ... most human genes is still barely understood. An important ... or protein is to compare its sequence with the ... easier to investigate. From the functions of related genes ...
Cached Biology News:Springer and Senckenberg society to collaborate on life sciences and geosciences journals 2New technique for cancer screening 2Relationships in rank and file 2Relationships in rank and file 3Relationships in rank and file 4
... the standard for sample concentration and drying. ... uses a combination of centrifugal force, heat and ... run. , SC210A comes equipped with:, ... chemical compatibility ...
... RC Maxi MB (for aqueous, other low ... evaporator systems respond to specific applications of ... safe concentration of heat-labile samples. The RC ... of cold traps and a wide selection ...
... The Jouan RC Standard Bio for ... applications of molecular biology and biochemistry for ... The RC systems can be expanded with ... wide selection of rotors (eliminating sample loss ...
... The Jouan RC10 series of vacuum concentrators/centrifugal ... protection features allied to rapid and safe ... can be combined with oil-free or high ... and a wide selection of rotors (eliminating ...
Biology Products: