Navigation Links
GC-RICH PCR System

for amplification of GC-rich targets (up to 5 kb) from genomic DNA Cat. No. 2 140 306 50 PCR reactions Description Unique enzyme blend of Taq DNA Polymerase and Pwo DNA Polymerase[a thermostable enzyme with a proofreading (3'5' exonuclease) activity].
The GC-RICH PCR System also includes:
  • An optimized GC-RICH PCR reaction buffer, 5x conc. containing 7.5 mM MgCl2 and DMSO
  • GC-RICH resolution solution, 5 M
  • MgCl2 stock solution, 25 mM
  • PCR grade water
Application The GC-RICH PCR System is designed to amplify difficult DNA templates up to 5 kb including:
  • GC-rich targets
  • Repetitive sequences
  • Templates with varying GC content (multiplex PCR, construction of random libraries)
Note: The GC-RICH PCR System may also be used in standard PCR, where it will give better results (higher yield and greater fidelity) than Taq DNA Polymerase alone. Application profile Operating parameters
  • Starting sample: 10500 ng DNA or 1100 ng cDNA
  • Temperature optimum (for elongation):
    - 72C (for targets < 3 kb)
    - 68C (for targets > 3 kb)
  • GC-RICH PCR System accepts modified nucleotides like DIG-dUTP
Experimental result Successfully amplify high GC-rich templates with the GC-RICH PCR System. Amplification of a 264 bp template (GC content 74%) within the human ApoE gene using the GC-RICH PCR System or the Expand High Fidelity PCR System. Key advantages
  • Allows amplification of difficult templates like GC-rich or repetitive sequences up to 5 kb, because the enzyme blend and the buffer provided with the system are optimized for these templates
  • Ideal for multiplex PCR, because the GC-RICH PCR System amplifies all targets with similar efficiency, regardless of GC content
  • Improves fidelity of PCR amplification, because the GC-RICH PCR System transcribes with threefold greater accuracy than Taq DNA Polymerase alone


'"/>

Source:


Page: All 1 2

Related biology technology :

1. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
2. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
3. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
4. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
5. Enhanced PCR Cloning System
6. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
7. Performance Comparisons of Commercial RT-PCR Systems
8. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
9. Yeast Protein Production System Features High Yields and One-Step Purification
10. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
11. Identification of Differentially Expressed Gene Products with the CastAway System*
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:6/23/2016)... 2016  The Prostate Cancer Foundation (PCF) is pleased to announce ... cures for prostate cancer. Members of the Class of 2016 were selected from ... Read More About the Class of 2016 PCF Young Investigators ... ... ...
(Date:6/23/2016)... , June 23, 2016   EpiBiome , a ... $1 million in debt financing from Silicon Valley Bank ... automation and to advance its drug development efforts, as ... facility. "SVB has been an incredible strategic ... services a traditional bank would provide," said Dr. ...
(Date:6/23/2016)... ... 23, 2016 , ... STACS DNA Inc., the sample tracking software company, today ... Laboratory, has joined STACS DNA as a Field Application Specialist. , “I am ... and COO of STACS DNA. “In further expanding our capacity as a scientific integrator, ...
(Date:6/23/2016)... June 23, 2016 Apellis Pharmaceuticals, Inc. ... clinical trials of its complement C3 inhibitor, APL-2. ... multiple ascending dose studies designed to assess the ... subcutaneous injection in healthy adult volunteers. ... as a single dose (ranging from 45 to ...
Breaking Biology Technology:
(Date:4/26/2016)... BANGALORE, India and LONDON ... Infosys Finacle, part of EdgeVerve Systems, a ... ), and Onegini today announced a partnership to ... banking solutions.      (Logo: http://photos.prnewswire.com/prnh/20151104/283829LOGO ... banks to provide their customers enhanced security to ...
(Date:4/19/2016)... UAE, April 20, 2016 The ... as a compact web-based "all-in-one" system solution for all ... fingerprint reader or the door interface with integration authorization ... access control systems. The minimal dimensions of the access ... into the building installations offer considerable freedom of design ...
(Date:4/15/2016)... -- Research and Markets has announced the ...  report to their offering.  ,      ... gait biometrics market is expected to grow at ... Gait analysis generates multiple variables such ... compute factors that are not or cannot be ...
Breaking Biology News(10 mins):