( for amplifica...)GC-RICH PCR System

for amplification of GC-rich targets (up to 5 kb) from genomic DNA Cat. No. 2 140 306 50 PCR reactions Description Unique enzyme blend of Taq DNA Polymerase and Pwo DNA Polymerase[a thermostable enzyme with a proofreading (3'5' exonuclease) activity].
The GC-RICH PCR System also includes:

• An optimized GC-RICH PCR reaction buffer, 5x conc. containing 7.5 mM MgCl₂ and DMSO
• GC-RICH resolution solution, 5 M
• MgCl₂ stock solution, 25 mM
• PCR grade water

Application The GC-RICH PCR System is designed to amplify difficult DNA templates up to 5 kb including:

• GC-rich targets
• Repetitive sequences
• Templates with varying GC content (multiplex PCR, construction of random libraries)

Note: The GC-RICH PCR System may also be used in standard PCR, where it will give better results (higher yield and greater fidelity) than Taq DNA Polymerase alone. Application profile Operating parameters

• Starting sample: 10500 ng DNA or 1100 ng cDNA
• Temperature optimum (for elongation):
  - 72C (for targets < 3 kb)
  - 68C (for targets > 3 kb)
• GC-RICH PCR System accepts modified nucleotides like DIG-dUTP

Experimental result Successfully amplify high GC-rich templates with the GC-RICH PCR System. Amplification of a
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