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GC-RICH PCR System

for amplification of GC-rich targets (up to 5 kb) from genomic DNA Cat. No. 2 140 306 50 PCR reactions Description Unique enzyme blend of Taq DNA Polymerase and Pwo DNA Polymerase[a thermostable enzyme with a proofreading (3'5' exonuclease) activity].
The GC-RICH PCR System also includes:
  • An optimized GC-RICH PCR reaction buffer, 5x conc. containing 7.5 mM MgCl2 and DMSO
  • GC-RICH resolution solution, 5 M
  • MgCl2 stock solution, 25 mM
  • PCR grade water
Application The GC-RICH PCR System is designed to amplify difficult DNA templates up to 5 kb including:
  • GC-rich targets
  • Repetitive sequences
  • Templates with varying GC content (multiplex PCR, construction of random libraries)
Note: The GC-RICH PCR System may also be used in standard PCR, where it will give better results (higher yield and greater fidelity) than Taq DNA Polymerase alone. Application profile Operating parameters
  • Starting sample: 10500 ng DNA or 1100 ng cDNA
  • Temperature optimum (for elongation):
    - 72C (for targets < 3 kb)
    - 68C (for targets > 3 kb)
  • GC-RICH PCR System accepts modified nucleotides like DIG-dUTP
Experimental result Successfully amplify high GC-rich templates with the GC-RICH PCR System. Amplification of a 264 bp template (GC content 74%) within the human ApoE gene using the GC-RICH PCR System or the Expand High Fidelity PCR System. Key advantages
  • Allows amplification of difficult templates like GC-rich or repetitive sequences up to 5 kb, because the enzyme blend and the buffer provided with the system are optimized for these templates
  • Ideal for multiplex PCR, because the GC-RICH PCR System amplifies all targets with similar efficiency, regardless of GC content
  • Improves fidelity of PCR amplification, because the GC-RICH PCR System transcribes with threefold greater accuracy than Taq DNA Polymerase alone


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