Navigation Links
GC-RICH PCR System

for amplification of GC-rich targets (up to 5 kb) from genomic DNA Cat. No. 2 140 306 50 PCR reactions Description Unique enzyme blend of Taq DNA Polymerase and Pwo DNA Polymerase[a thermostable enzyme with a proofreading (3'5' exonuclease) activity].
The GC-RICH PCR System also includes:
  • An optimized GC-RICH PCR reaction buffer, 5x conc. containing 7.5 mM MgCl2 and DMSO
  • GC-RICH resolution solution, 5 M
  • MgCl2 stock solution, 25 mM
  • PCR grade water
Application The GC-RICH PCR System is designed to amplify difficult DNA templates up to 5 kb including:
  • GC-rich targets
  • Repetitive sequences
  • Templates with varying GC content (multiplex PCR, construction of random libraries)
Note: The GC-RICH PCR System may also be used in standard PCR, where it will give better results (higher yield and greater fidelity) than Taq DNA Polymerase alone. Application profile Operating parameters
  • Starting sample: 10500 ng DNA or 1100 ng cDNA
  • Temperature optimum (for elongation):
    - 72C (for targets < 3 kb)
    - 68C (for targets > 3 kb)
  • GC-RICH PCR System accepts modified nucleotides like DIG-dUTP
Experimental result Successfully amplify high GC-rich templates with the GC-RICH PCR System. Amplification of a 264 bp template (GC content 74%) within the human ApoE gene using the GC-RICH PCR System or the Expand High Fidelity PCR System. Key advantages
  • Allows amplification of difficult templates like GC-rich or repetitive sequences up to 5 kb, because the enzyme blend and the buffer provided with the system are optimized for these templates
  • Ideal for multiplex PCR, because the GC-RICH PCR System amplifies all targets with similar efficiency, regardless of GC content
  • Improves fidelity of PCR amplification, because the GC-RICH PCR System transcribes with threefold greater accuracy than Taq DNA Polymerase alone


'"/>

Source:


Page: All 1 2

Related biology technology :

1. New Mammalian Two-Hybrid System Detects Protein-Protein Interactions
2. Assess the In Vivo Activation of Signal Transduction Pathways with PathDetect Reporting Systems
3. Generate Adenovirus Vectors in E. coli by Homologous Recombination with the AdEasy Adenoviral Vector System
4. New Yeast Cloning System for Producing Proteins with Native Amino Acid Sequences
5. Enhanced PCR Cloning System
6. prostar RT-PCR Systems for Robust High-Fidelity RNA Amplification
7. Performance Comparisons of Commercial RT-PCR Systems
8. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
9. Yeast Protein Production System Features High Yields and One-Step Purification
10. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
11. Identification of Differentially Expressed Gene Products with the CastAway System*
Post Your Comments:
*Name:
*Comment:
*Email:


(Date:2/24/2017)... ... February 24, 2017 , ... A ... safely completed in an ambulatory surgery center (ASC) with satisfactory clinical and patient-reported ... two-year TDR studies. , Jake Lubinski, president of AxioMed, commented on the ...
(Date:2/24/2017)... -- Symic Bio, a biopharmaceutical company focused on matrix ... announced today the completion of enrollment for the SHIELD ... trial will evaluate the safety and efficacy of SB-030, ... restenosis following angioplasty. "We,re pleased to ... Nathan Bachtell , M.D., Chief Medical Officer of ...
(Date:2/24/2017)... 24, 2017  VWR Corporation (NASDAQ: VWR), the leading global ... production customers, today reported its financial results for the fourth ... Highlights: 4Q16 record quarterly net sales ... an organic basis. 4Q16 EMEA-APAC ... basis, while the Americas net sales increased 2.5%, or down ...
(Date:2/23/2017)... The Fight Against Cancer Innovation Trust (FACIT) and the ... report that Fusion Pharmaceuticals Inc. (Fusion) has closed a ... – JJDC, Inc. (JJDC) as the lead investor. Additional, ... and Genesys Capital, as well as founding investor FACIT.  ... ...
Breaking Biology Technology:
(Date:2/3/2017)... 3, 2017 A new independent identity strategy ... LLP (IdSP) . Designed to fill a critical niche ... market, founding partners Mark Crego and ... just in identity expertise that span federal governments, the ... The Crego-Kephart combined expertise has a common theme born ...
(Date:2/2/2017)... 2017  EyeLock LLC, a market leader of iris-based ... " What You Should Know About Biometrics in the ... authenticity is a growing concern. In traditional schemes, cryptography ... traditional authentication schemes such as username/password suffer from inherent ... offers an elegant solution to the problem of high-security ...
(Date:2/1/2017)... Massachusetts , February 1, 2017 IDTechEx ... events on emerging technology, announces the availability of a new report, ... Continue Reading ... ... in industrial and collaborative robots. Source: IDTechEx Report "Sensors for Robotics: ...
Breaking Biology News(10 mins):