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Fundamentals of Fermentation: Techniques For Benchtop Fermentors

ould be about 1 cm below the filling orifice. To add solution, see pH Probe Preparation.
2. Check for any trapped air bubbles in the electrode's tip to remove bubbles, hold electrode upright and shake electrode gently.
3. The probe should be stored standing upright. The electrode tip should be immersed in the solution of 3 molar KC1 or a buffer solution between pH 4 and pH 7. The two rubber "T" stoppers should be inserted. At no time should the electrode be allowed to rest on the tip.

pH PROBE CALIBRATION
Note: pH electrode is calibrated before autoclaving vessel.
1. Connect electrode to the pH connector using the appropriate cable.
2. Turn ON the main power switch.
3. Display calibration screen.

Note: The pH measuring system is calibrated using two external buffer solutions of known pH.
4. Immerse pH electrode into pH 7.0 buffer solution and allow a few minutes for the system to equilibrate.
5. Set the pH function to Zero.
6. Set the display to read 7.00.
7. Rinse the pH electrode with distilled water.
8. Immerse pH electrode into a second pH buffer solution which is several pH units above or below pH 7.00 (e.g. 4.00) and allow a few minutes for the system to equilibrate
9. Set the pH function to "Span".
10. Set the display to read the value of the second buffer solution.
11. Repeat steps 5 - 10 using the same buffer solutions.

Note: The pH calibration should be checked after autoclaving immediately prior to inoculation. This is performed by taking a sample from the vessel and
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