( detergent or by using a cleaningsol...)Fundamentals of Fermentation: Techniques For Benchtop Fermentors

detergent, or by using a cleaning solution. If the vessel is to be sterilized, all standard precautions must be taken. Note that for this purpose, the vessel does not need to be sealed except for those previously cited valves and tubing which run under the liquid level. It will be necessary to use water in the vessel. We recommend the use of DI water, and the fill should be at least as high as your standard level for a run. Unless you have already specifically wiped the residual grease off the top of the glass cylinder, there should be enough so that the headplate can be clamped to the lower portion of the vessel. Note that it is neither necessary nor desirable to fasten these clamps with the same force used for attaching the headplate prior to a run, as this could lead to vessel damage. Instead, the lightest possible pressure should be used. The advantage to sterilization is that not only are residual viable organisms killed, but residual debris will loosen and become removable by washing after the vessel has cooled. If a cleaning solution is required, we recommend a 10% dilution of Micro cleaning solution (International Products Corporation, catalog number 6732). Alternatively, if you are using the vessel for consecutive runs with the same media, a rinsing of it with warm tap water and the DI water may suffice. Note that if water will run over a vessel surface that has grease on it, the grease should be removed by wiping it off with a wet paper towel.

In cases where the vessel must be decontaminated prior to cleaning, add water so that the liquid level reaches the maximum working volume of the vessel. This will help prevent biological materials from adhering.

APPENDIX A.
BIOFLO 3000 Probes:

pH PROBE MAINTENANCE AND STORAGE
1. Check the level of the filling solution. It sh
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