( the sampler should be removed and rins...)Fundamentals of Fermentation: Techniques For Benchtop Fermentors

the sampler should be removed and rinsed separately. The glass wool can be removed at this point as well. The sampling tube is detached and washed separately. The valve on the sampling port and all clamps on all tubing connected to ports will need to be open for proper washing. (Note that the media will need to be removed prior to unclamping any tubing that is below the liquid level, as is the case with a side harvest line). The headplate should be detached by loosening and then removing the clamps that hold it to the rest of the vessel. Those clamps may require rinsing. The remaining culture broth should be sterilized or emptied into a bucket and disinfected by using bleach or other accepted disinfectant prior to disposal. Note that some media may be incompatible with this procedure, in which case the media can be placed into another container for sterilization prior to disposal. The headplate should be washed thoroughly with warm water and then de-ionized (DI) water. It may be necessary to scrub accumulations of biomaterial off. A pad that won't scratch the steel is required for this. The agitation shaft, the thermowell, harvest and sparger tubes and the short beveled tips of the interior portion of the base-type addition ports will often require special attention. All tubes and shafts must be cleaned. Note that there may be some residual base or acid left in those lines, so extreme caution and the use of chemically-resistant gloves is recommended for this procedure. It is often necessary to hand wipe surfaces with a paper towel in order to fully remove residual traces of small particulate debris.

The washing of the bottom portion of the vessel requires the same procedures as the headplate. Note that the sides of the vessel, particularly where the baffle was adjacent to it, and side ports (plugged or unplugged) may require special attention.

The vessel can now be cleaned by washing with
'"/>

Source:

Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19
Related biology technology :

1. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
2. P. pastoris Fermentation using a BioFlo 110 Benchtop Fermentor
3. CHO Cell Culture Application Using a BioFlo 110 Benchtop Bioreactor
4. Insect Cell Culture Using a BioFlo 110 Benchtop Bioreactor
5. Hybridoma Cell Culture Application Using a BioFlo 110 Benchtop Bioreactor
6. High Yield Protein Production from Pichia pastoris Yeast: A Protocol for Benchtop Fermentation