( probedown more vigorously but in no ...)Fundamentals of Fermentation: Techniques For Benchtop Fermentors

probe down more vigorously but in no case should the tip be touched. After cleaning the D.O. probe the tip can be visually inspected for damage. The probe should then be stored in a clean area in such a way as to protect the sensitive tip. Removing the pH probe is usually not as difficult a process as inserting it because the shaft will be wet and thus should be relatively easy to remove. However the danger of probe breakage is still very real and extreme care must be taken when removing it. It is still necessary to use two hands with one hand at the top of the port acting as a guide to ensure proper removal. A gentle pace for this is required. If at any point in this process the probe jams, it is essential to avoid trying to force it. It may be necessary to reinsert it part way and apply a lubricant such as glycerin to the shaft and port in order to effect the probes' removal. In extreme cases it may be necessary to remove the headplate with the probe still inside so that you can approach the problem from both ends. In this case, it is critical to remove the headplate very carefully. (We recommend that you have a spare probe available at all times, in case of breakage.) Once the pH probe has been removed, it should be immediately washed off with warm water. If biomaterial has accumulated on the probe, using a sponge (or an equivalent that will not scratch glass) and gentle pressure to clean the surface. The very tip of the probe should be handled with extreme care and a Kimwipe should be used to gently dry it off after washing. The probe should be stored with the tip immersed in either electrolyte or pH 7 buffer. This electrolyte/buffer can be reused, but it should always be inspected prior to each use for precipitation or contamination.

Now that the vessel is detached from the unit, it can be cleaned. To do this it will be necessary to remove any remaining cotton and foil covering the ports. The rubber bulb on
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