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Fully automated DNA purification and efficient multiplex PCR for analysis ,,, of microsatellite loci

Marion Schmidt, Holger Engel, Markus Sprenger-Hauels, Corinna Kppers, Gaby Schulte, Silke Zobel, and Dirk Lffert
QIAGEN GmbH, Hilden, Germany

Microsatellites highly polymorphic DNA markers comprised of nucleotides that are repeated in tandem arrays and distributed throughout the genome are a useful tool for genetic identification of individuals. The microsatellites most frequently used in PCR analysis are short tandem repeats (STRs). Typical applications for the analysis of STRs are forensics, identity and paternity testing, and genetic-linkage analysis. In such investigations, simultaneous analysis of multiple microsatellite loci by multiplex PCR is highly desirable.

Important factors for successful multiplex PCR amplification are the quality and concentration of PCR template. For medium and high throughput, automated methods provide efficient nucleic acid purification with minimal handling variability, leading to more consistent results. The BioRobot MDx workstation provides walkaway automated purification of genomic and mitochondrial DNA from blood using QIAGEN certified ready-to-run QIAamp protocols. The protocol is rapid and efficient, requiring only 2.5 hours preparation time for 96 samples (including a detailed load check of 20 minutes). Automated reaction setup for downstream applications ensures maximal reliability.

The new QIAGEN Multiplex PCR Kit is the first commercially available kit specifically developed for multiplex PCR and provides a fast, efficient, and reliable procedure. The simple multiplex master-mix
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