Navigation Links
Fully Automated Nano-Electrospray Coupled with a Finnigan LCQ Deca XP Plus for Sub-fmol Proteomic Analysis

ter which accepts only peptides with Xcorr values of 1.5, 2.0, and 2.5 and higher for singly, doubly, or triply charged precursor ions.

Results and Discussion
The tryptic digest of horse myoglobin was analyzed by nanospray liquid chromatography as described in the Experimental section. The LC/MS system was run under standard conditions one would use for typical protein identification in a complex mixture of proteins and not optimized for the detection of horse myoglobin digest. The mass range for full mass spectrum was 400 Da to 1500 Da and each full mass spectrum was followed by three MS/MS scans of the most intense ions. Dynamic Exclusion was enabled. Horse myoglobin could be identified in amounts as low as 500 amol. Figure 3 shows the BioWorks results and Figure 4 shows one of two tandem mass spectra obtained for this low concentration. Both tandem mass spectra contained sufficient fragment ions for positive identification by the SEQUEST algorithm. The results were confirmed several times after rigorous washing of the LC (peptide trapPicoFrit column) system.

The Finnigan Surveyor HPLC system, used with an optimized flow splitting system, generated very reproducible chromatograms at flow rates of 100 nL/min. Two different amounts (250 fmol and 500 fmol) of a tryptic myoglobin digest were analyzed three time each and the elution time of each peptide differs by less than 10 seconds from run to run, which is less than 0.3% of the elution time (Figure 5).

Overall system reproducibility was further evaluated by analyzing a mixture of all five standard protein digests at various concentrations ranging from 10 fmols to 6 pmols. Forty replicate analyses were performed. All five proteins could be identified in each of the 40 analyses, using BioWorks software. Peptide coverage for each protein was very similar between all runs, demonstrating excellent reproducibility of LC/MS with t
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci
2. Purified Single-Stranded M13 DNA for Automated Sequencing
3. Automated Fluorescent-Tag Cycle Sequencing
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
6. Agilent 2100 Bioanalyzer Automated Analysis System
7. Automated RNA Amplification for Arrays
8. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
9. Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A
10. Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents
11. DNA Damage and Repair - Quantification of Sub-Cellular Events Using Automated Confocal Imaging
Post Your Comments:
(Date:12/24/2014)... December 23, 2014 PharmaBoardroom,s ... out today and available for free download , digs ... change and growth in the sector today. One ... successes has been in developing a homegrown pharmaceutical manufacturing base, ... production still remains some way off. A cursory comparison with ...
(Date:12/24/2014)... , Dec. 23, 2014  NeuroLifeSciences announced today ... in children with attention deficit/hyperactivity disorder published in ... The paper, titled "Pilot Phase 2 ... ( Konofal et al, Drug Des Devel ... shows that mazindol might be an effective, well-tolerated, ...
(Date:12/22/2014)... /PRNewswire/ - RepliCel Life Sciences Inc. (OTCQB: REPCF) (TSX.V: ... the development of autologous cell therapies, announced today the ... Calgary in conjunction with co-authors from Kyoto University and ... company,s ongoing clinical research using dermal sheath cup (DSC) ... The paper entitled " Hair Follicle Dermal Stem ...
(Date:12/22/2014)... 22, 2014  ( www.competitivehealth.com ) — Competitive Health ... bill review and advocacy service, has signed an agreement ... Savings discount health services marketplace. 63% of ... than they expected to pay. As part of an ... WellCard Savings is pleased to offer medical bill review ...
Breaking Biology Technology:PharmaBoardroom Releases New Algeria Pharmaceuticals Report 2PharmaBoardroom Releases New Algeria Pharmaceuticals Report 3Redefining ADHD: A New Approach & A Shift of Paradigm in ADHD Therapeutics 2Redefining ADHD: A New Approach & A Shift of Paradigm in ADHD Therapeutics 3Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 2Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 3Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 4Newly Released Peer-Reviewed Publication Further Validates RepliCel's Use of Dermal Sheath Cup Cells to Treat Pattern Baldness 5WellCard Savings Announces CoPatient Medical Bill Review Service for Members 2
... Fond du Lac, Wis. -- Careers in manufacturing are ... be on the horizon. It appears a negative public ... assembly-line work and lay-offs - is thwarting young adults ... a projected need for 10 million new skilled workers ...
... Madison, Wis. - The Department of Commerce (Commerce) ... under the new Angel Investor and Venture Fund Tax ... to make these crucial investments, we are helping to ... Jim Doyle said. , ,The Angel Investor and ...
... low prices. When it comes to consumer goods, America is ... fanatical commitment to low prices, Wal-Mart is the biggest low-price ... the place to get it. , ,The company that many ... lately. Union and activist groups have successfully derailed its growth ...
Cached Biology Technology:State campaign seeks to reverse negative perception of manufacturing work 2State campaign seeks to reverse negative perception of manufacturing work 3State campaign seeks to reverse negative perception of manufacturing work 4State campaign seeks to reverse negative perception of manufacturing work 5Five technology companies qualify for investor tax credits 2Wal-Mart: How low can you go? 2Wal-Mart: How low can you go? 3Wal-Mart: How low can you go? 4
(Date:12/5/2014)... , Dec. 4, 2014 Increased ... boosted investments in new testing and inspection technologies. ... given rise to a range of innovative test ... requirements of Generation Y, which is generally more ... a result, product development strategies of test equipment ...
(Date:11/21/2014)... , November 18, 2014 According ... Market by Systems (Video, RFID, Access Control, Intrusion Detection, ... Hotels, Banks, Government), Component Service Geography - Global Forecasts ... Market is projected to be around $25 Billion in ... 2020, growing at a CAGR of 8.69%. ...
(Date:11/18/2014)... , Nov. 18, 2014  The Secure Identity ... Association today jointly announce the formation of The ... of its Identity and Biometric Entry and ... Framework received official support from BORDERPOL, the international ... improve and provide expertise regarding border security, traveler ...
Breaking Biology News(10 mins):Emerging Technologies Enable Smarter Monitoring of Assets, Push Industrial Testing, Inspection and Monitoring Market 2Emerging Technologies Enable Smarter Monitoring of Assets, Push Industrial Testing, Inspection and Monitoring Market 3Emerging Technologies Enable Smarter Monitoring of Assets, Push Industrial Testing, Inspection and Monitoring Market 4Industrial Security Systems Market Worth $38 Billion by 2020 2Industrial Security Systems Market Worth $38 Billion by 2020 3Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 2Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 3
... for their future needs just as we humans can ... and Helena Osvath,s research, from Lunds University Cognitive Science ... of advanced planning capacities in non-human species. Their findings ... Animal Cognition . The complex skill of future ...
... The world,s only known hairy-nosed otter in captivity, one of the ... and a Buddhist blessing today. ... killed by a fisherman, was released into a large new enclosure ... located near Phnom Penh. The release was celebrated with a ...
... be the early targets of new trans-Pacific research using ... of Queensland (UQ) and the University of Washington (UW) ... Anna Bligh announced $650,000 from the National and International ... of the pathogens of two debilitating mosquito-borne diseases, malaria ...
Cached Biology News:Great apes think ahead 2World's only captive hairy-nosed otter gets new home 2World's only captive hairy-nosed otter gets new home 3
Kit includes your choice of reagents for labeling 3 x 10 9 cells and EasySep magnet....
... stabilizing 1.5mg of purified RNA samples., , ... stabilization of aqueous RNA samples that have ... guanidine, phenol/chloroform, silica, oligo dT etc or ... all the necessary components for the stabilization ...
Blasticidin resistance gene (bsr), provided in a pORF plasmid. pORF is an expression plasmid selectable with Ampicillin....
Plasmid expressing Zeocin resistance gene....
Biology Products: