Navigation Links
Fully Automated Nano-Electrospray Coupled with a Finnigan LCQ Deca XP Plus for Sub-fmol Proteomic Analysis

Dirk Chelius, Terry Zhang, and Ken Miller;
Thermo Electron Corporation, San Jose, CA

Key Words Sensitivity Proteomics Finnigan LCQ Deca XP Plus PepFinder Kit Finnigan Surveyor HPLC LC/MS/MS

Introduction
Detection limits of mass spectrometers have been pushed downward to levels no one would have thought possible only a decade ago. Sensitivity gains are driven by instrument hardware improvements and sophisticated methods of sample introduction to the mass analyzer. Nanoflow ESI is a very popular method in proteomics to achieve maximum sensitivity and separation of peptides. Peptides are separated on a capillary reversed-phase column (75 μm internal diameter [ID]), which is placed directly in front of the mass spectrometer to limit peak broadening after the column. For many reasons, use of these small ID columns can be complex. Engineering an optimal solution to these practical issues can be very time consuming. The optimal flow rate for a 75 μm ID column is between 100 and 200 nL/min, often requiring flow splitting. At such low flow rates, loading of a sample onto the column can take a long time: at a flow rate of 100 nL/min it takes 200 minutes to elute a 20 μL sample loop. Researchers have attempted to overcome this problem by loading the sample onto the column during the packing of the column, but this method is very time consuming and each column can only be used one time.Alternative approaches are the loading of the samples at a higher flow rate or the use of a peptide trap. The peptide trap has several advantages compared to other loading techniques; peptides can be loaded at a higher flow rate, desalted and washed. This combination allows high sample throughput and maximal sensitivity of detection. This plumbing configuration is the basis of the PepFinder Kit. Designed for easy installation and maximum productivity, all PepFinder Kit componen
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci
2. Purified Single-Stranded M13 DNA for Automated Sequencing
3. Automated Fluorescent-Tag Cycle Sequencing
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
6. Agilent 2100 Bioanalyzer Automated Analysis System
7. Automated RNA Amplification for Arrays
8. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
9. Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A
10. Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents
11. DNA Damage and Repair - Quantification of Sub-Cellular Events Using Automated Confocal Imaging
Post Your Comments:
(Date:5/23/2013)... CAMBRIDGE, England , May 24, 2013 ... von Mundipharma angefertigt – Bei der ... Version , 91 % der Teilnehmer einer ... obwohl nur 20 % laut GINA [*] -Definition [1] ... zeigt, dass Menschen mit Asthma generell einer von vier ...
(Date:5/23/2013)... Can economic incentives such as gift cards, ... the public to increase their donations of blood? , ... Business School Assistant Professor Mario Macis says the ... body of recent research that supports their argument, the ... of Science that the World Health Organization and national ...
(Date:5/23/2013)... , May 23, 2013  Rahal Letterman Lanigan Racing ... cord blood stem cell banking and therapeutics, announced today ... No. 16 Acorn Stairlifts Indy car driven by ... remainder of the IZOD IndyCar 2013 season.   ... work to support awareness of umbilical cord blood banking ...
(Date:5/23/2013)... , May 23, 2013  Saddleback Memorial Medical Center ... became the first hospital on the West Coast to ... new surgical option provides women with a state-of-the-art, minimally ... Marc Winter , M.D., a highly regarded ... and medical director of minimally invasive surgery at Saddleback ...
Breaking Biology Technology:Neue Forschungsergebnisse zeigen Verbindung zwischen suboptimaler Asthmaversorgung und Patienteneinstellungen in Europa auf 2Neue Forschungsergebnisse zeigen Verbindung zwischen suboptimaler Asthmaversorgung und Patienteneinstellungen in Europa auf 3Neue Forschungsergebnisse zeigen Verbindung zwischen suboptimaler Asthmaversorgung und Patienteneinstellungen in Europa auf 4Neue Forschungsergebnisse zeigen Verbindung zwischen suboptimaler Asthmaversorgung und Patienteneinstellungen in Europa auf 5Neue Forschungsergebnisse zeigen Verbindung zwischen suboptimaler Asthmaversorgung und Patienteneinstellungen in Europa auf 6Neue Forschungsergebnisse zeigen Verbindung zwischen suboptimaler Asthmaversorgung und Patienteneinstellungen in Europa auf 7Offering Economic Incentives to Attract Blood Donations Should Be Encouraged, Research Team writes in Science 2Offering Economic Incentives to Attract Blood Donations Should Be Encouraged, Research Team writes in Science 3StemCyte, Inc. Joins Rahal Letterman Lanigan Racing As Associate Sponsor Of No. 16 IndyCar Program For James Jakes Beginning With The 97th Indianapolis 500 2StemCyte, Inc. Joins Rahal Letterman Lanigan Racing As Associate Sponsor Of No. 16 IndyCar Program For James Jakes Beginning With The 97th Indianapolis 500 3Saddleback Memorial Surgeon First on the West Coast to Perform Single-Site Robotic Hysterectomy 2Saddleback Memorial Surgeon First on the West Coast to Perform Single-Site Robotic Hysterectomy 3
... (Euronext: AMT), a leader in the field of human ... for personal reasons retire,as chief executive officer, effective February ... and co-founder of the company, has,been named chief executive ... Since Ronald Lorijn started in 2005, AMT changed ...
... Fred Hutchinson Cancer Research Center,s,stem-cell transplant program at ... only two transplant programs nationwide that consistently outperformed,the ... according,to an independent report that assessed 122 transplant ... The Center for International Blood and Marrow ...
... products are featured in Genetic Engineering News for delivering the promise ... ... Fort Collins, CO (PRWEB) January 22, 2009 -- InVitria,s product line ... News (GEN, Jan 15, 2009 pp 32-34). The article, entitled ...
Cached Biology Technology:AMT CEO Ronald Lorijn Steps Down 2AMT CEO Ronald Lorijn Steps Down 3Transplant Program Tops in Expected One-Year Survival Rate 2InVitria Among Leading Innovators in Cell Culture Media According to Top Biotechnology Publication 2
(Date:5/22/2013)... technique that can detect molecular variants in chemical mixtures ... the most important, though time-consuming, processes in analytical chemistry. ... Nature , post-doctoral researcher David Patterson, Professor of Physics ... for Free-Electron Laser Science (CFEL) in Hamburg, Germany developed ... identify molecular variants apart, and to determine how much ...
(Date:5/22/2013)... of potential environmental and human health effects from ... year has led scientists to recommend stronger government ... lithium-ion (Li-ion) battery materials. That,s the conclusion of ... Environmental Science & Technology . , Oladele A. ... have become mainstays for powering everything from smart ...
(Date:5/22/2013)... 22, 2013 Early screening for prostate cancer could ... is for women, thanks to UC Irvine research published ... Society . , After more than a decade of ... clearly identify clinically usable markers for prostate cancer in ... sooner, with greater accuracy and at dramatically lower cost. ...
Breaking Biology News(10 mins):Detecting mirror molecules 2Detecting mirror molecules 3UCI chemists devise inexpensive, accurate way to detect prostate cancer 2UCI chemists devise inexpensive, accurate way to detect prostate cancer 3
... LAFAYETTE, Ind. - A group of Purdue University researchers has ... materials, such as nutrients and drug treatments, to move in ... Jue Chen, an associate professor of biological sciences, obtained a ... closes pathways through the protective cellular membrane. The gates, operated ...
... CONRAD Program of the Eastern Virginia Medical School today announced ... Bill & Melinda Gates Foundation to develop microbicides for HIV ... total of $65 million to CONRAD for microbicide research. ... combination microbicides, research on novel and improved in vitro and ...
... at Duke University have created the first map of imprinted ... Rosetta stone a form of artificial intelligence called machine ... The study revealed four times as many imprinted genes as ... of the December 3 issue of Genome Research. In ...
Cached Biology News:Purdue researchers obtain a snapshot clarifying how materials enter cells 2Purdue researchers obtain a snapshot clarifying how materials enter cells 3CONRAD receives $28.5M Gates Foundation grant for HIV prevention research 2Duke scientists map imprinted genes in human genome 2Duke scientists map imprinted genes in human genome 3
The Captivate Disposable Sample Chamber is designed for use with the Captivate magnetic yoke assembly (C-24700) and can be used with our Captivate ferrofluid conjugates (C-21473 C-21474 C-21476) or ...
MOUSE ANTI HUMAN MOC31:AZIDE FREE...
... mycobacteria in Complete Freund's adjuvant attract macrophages ... which enhances the immuneresponse. For this reason, ... the initial injections; Incomplete Freund's adjuvant is ... in saline) are typically mixed with an ...
HiTrap Streptavidin HP, 5 x 1 ml. Category: Chromatography Systems & Accessories, Systems....
Biology Products: