Navigation Links
Fully Automated Nano-Electrospray Coupled with a Finnigan LCQ Deca XP Plus for Sub-fmol Proteomic Analysis

Dirk Chelius, Terry Zhang, and Ken Miller;
Thermo Electron Corporation, San Jose, CA

Key Words Sensitivity Proteomics Finnigan LCQ Deca XP Plus PepFinder Kit Finnigan Surveyor HPLC LC/MS/MS

Introduction
Detection limits of mass spectrometers have been pushed downward to levels no one would have thought possible only a decade ago. Sensitivity gains are driven by instrument hardware improvements and sophisticated methods of sample introduction to the mass analyzer. Nanoflow ESI is a very popular method in proteomics to achieve maximum sensitivity and separation of peptides. Peptides are separated on a capillary reversed-phase column (75 μm internal diameter [ID]), which is placed directly in front of the mass spectrometer to limit peak broadening after the column. For many reasons, use of these small ID columns can be complex. Engineering an optimal solution to these practical issues can be very time consuming. The optimal flow rate for a 75 μm ID column is between 100 and 200 nL/min, often requiring flow splitting. At such low flow rates, loading of a sample onto the column can take a long time: at a flow rate of 100 nL/min it takes 200 minutes to elute a 20 μL sample loop. Researchers have attempted to overcome this problem by loading the sample onto the column during the packing of the column, but this method is very time consuming and each column can only be used one time.Alternative approaches are the loading of the samples at a higher flow rate or the use of a peptide trap. The peptide trap has several advantages compared to other loading techniques; peptides can be loaded at a higher flow rate, desalted and washed. This combination allows high sample throughput and maximal sensitivity of detection. This plumbing configuration is the basis of the PepFinder Kit. Designed for easy installation and maximum productivity, all PepFinder Kit componen
'"/>

Source:


Page: All 1 2 3 4 5

Related biology technology :

1. Fully automated DNA purification and efficient multiplex PCR for analysis of microsatellite loci
2. Purified Single-Stranded M13 DNA for Automated Sequencing
3. Automated Fluorescent-Tag Cycle Sequencing
4. CastAway Precast Gels for Rapid Automated DNA Sequence Analysis
5. The DIG System Nonradioactive Automated High-Throughput In Situ Hybridization: a Powerful Tool for Functional Genomics Research
6. Agilent 2100 Bioanalyzer Automated Analysis System
7. Automated RNA Amplification for Arrays
8. Quantitation of DNA for Automated Sequencing Using the VersaFluor Fluorometer
9. Performance Comparison of the Experion Automated Electrophoresis System and a Competing Automated System for Protein Analysis, Rev A
10. Total RNA Purification from Cultured Cells Using the ABI PRISM 6700 Automated Nucleic Acid Workstation and Total RNA Lysis Reagents
11. DNA Damage and Repair - Quantification of Sub-Cellular Events Using Automated Confocal Imaging
Post Your Comments:
(Date:1/15/2014)... , Jan. 15, 2014 ­ RedBrick Health , ... change technology, today announces that EmblemHealth , the ... wellness company, is now providing the RedBrick Compass health ... program, to all of its members. EmblemHealth is among ...
(Date:1/15/2014)... Rochester, NY (PRWEB) January 15, 2014 The ... cause a major disease. One of these latent viruses is ... is rheumatoid arthritis (RA). Rheumatoid arthritis (RA) is a chronic ... the theory, a study found that RA patients have high ...
(Date:1/15/2014)... TaiGen Biotechnology Company, Limited ("TaiGen") today announced that ... leading Russian pharmaceutical company, to develop and commercialize nemonoxacin ... , Turkey and other ... novel antibiotic for the treatment of bacterial infections including ...
(Date:1/14/2014)... 2014 As pet owners drew up their lists of New ... "Take better care of my furry companion." Nowadays there are pet ... carrying cases to take the little canine or feline darlings along ... to buy some pricey toys at the pet store. But anyone ...
Breaking Biology Technology:EmblemHealth Selects RedBrick Health to Power its Next-Generation Health Engagement and Behavior Change System 2EmblemHealth Selects RedBrick Health to Power its Next-Generation Health Engagement and Behavior Change System 3Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 2Study: Rheumatoid Arthritis (RA) Patients Have EBV; The CBCD Says this is Consistent with Microcompetition 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 2TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 4New Year's Resolution: Give Pets the Gift of Top-Notch Health Care 2
... MOUNTAIN VIEW, Calif., Feb. 17 Frost & Sullivan, ... is,regaining its strength, thanks in part to an unprecedented ... additional stimulus packages from,foreign governments. , ... "President Obama,s stimulus package is clearly serving ...
... Warwick have found that tiny nanoparticles could be twice ... non mixing liquids than previously believed. This opens ... of nanoparticles in living cells, polymer composites, and ... working on ways of further artificially enhancing this new ...
... LLC, a wholly owned subsidiary of The Dow Chemical ... Rice Research Institute (CNRRI) of Hangzhou,China, announced today that ... strengths of Dow AgroSciences, platform of traits and technologies,with ... "We look forward to working with CNRRI to introduce ...
Cached Biology Technology:Frost & Sullivan Forecasts Early Rebound: Strong Growth Ahead for Global Economy 2Nanoparticles double their chances of getting into sticky situations 2Dow AgroSciences, China National Rice Research Institute Announce Collaboration on Rice Research 2
(Date:4/17/2014)... and pathogens that devastate honeybees in Europe, Asia and ... do not appear to be impacting native honeybee populations ... researchers., The invasive pests include including Nosema microsporidia ... honeybees appear to be resilient to these invasive pests, ... control pests in Europe, Asia and the United States ...
(Date:4/17/2014)... implicated in many serious conditions such as heart disease, ... for drug therapies. Unfortunately, there is still much scientists ... provides fresh and unexpected insight into the structure of ... β-subunit molecules - which are responsible for ,fine-tuning, the ... the most recent edition of the Journal of ...
(Date:4/17/2014)... -- Indiana University researchers have detected new early-warning signs ... This discovery could have far-reaching implications for the diagnosis ... of over 25 million Americans. , "We had not ... at such early stages," said Ann Elsner, professor and ... lead author of the study. "We set out to ...
Breaking Biology News(10 mins):East African honeybees are safe from invasive pests… for now 2East African honeybees are safe from invasive pests… for now 3Structure of sodium channels different than previously believed 2New technique detects microscopic diabetes-related eye damage 2New technique detects microscopic diabetes-related eye damage 3
... pro-inflammatory signaling molecule involved in immune response in ... are doing" in creating drugs based on a ... Oct. 1 issue of the Journal of Immunology, ... unveiled the crystal structure of mouse interleukin-1 receptor-associated ...
... , The annual Educational Technology Award of the ... the project 'Virtual microscopy as a teaching aid' of ... awarded project utilizes advanced digital imaging and network technology ... internet. , 'Virtual specimens' incorporated into educational applications ...
... into the evolutionary process by examining how albinism ... University Biology Professor Richard Borowsky, examined two populations ... both populations was linked to Oca2--a pigmentation gene ... albinism in humans. They observed different deletions in ...
Cached Biology News:Molecular research suggest shift needed in how some drugs are created 2Genetic analysis of cavefish reveals more about evolution 2
For relative and/or absolute quantification with standard accuracy requirements using MS or NMR instruments...
Carrier Serum...
A Highly Purified, Cost-Effective Taq DNA Polymerase...
Homo sapiens similar to Cytosolic acyl coenzyme A thioester hydrolase Antigen: Peptide...
Biology Products: