Navigation Links
Front-face detection for high-concentrate, opaque or solid sample

When working with highly-concentrated, opaque, or solid samples such as blood, paint, optical brighteners, living cells, polymer films, or phosphors, front-face fluorescence detection offers significant advantages for quantitative and qualitative fluorescence measurements. Highly concentrated and opaque liquids typically have problems with self-absorption and complete attenuation of the beam. When measuring fluorescence at 90, intensity measurements may not be reproducible or detectable, and the excitation or emission spectra may appear distorted. Front-face detection offers the solution for these problems.

Using the technique of front-face detection for opaque liquids and solids can be an important analytical tool for characterizing fluorescence of various sample types. In the front-face technique, the excitation light is focused to the front surface of the samples and then fluorescence emission is collected from the same region at an angle that minimizes reflected and scattered light.

Types of Samples

Highly concentrated: blood, oil, optical brighteners
Highly opaque: paints, phosphors
Solids: polymers, films, paper, contact lenses, crystals

Front-Face Detection

FLUOROLOG Use the 22.5 collection path for liquid and solid samples.
FLUOROMAX Use the Model 1933 Solid-Sample Holder at a 30/60 angle for solids. For liquids, use the Model 1967 Front-Face Thermostatted Cell Holder with magnetic stirrer accessory.

back to top


Page: All 1

Related biology technology :

1. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
2. Apoptosis detection by annexin V and active caspase-3 with the Agilent 2100 bioanalyzer
3. Sensitive detection of tumor cells in peripheral blood of carcinoma patients by a reverse transcription PCR method
4. Rapid detection of genomic duplications and deletions using the Agilent 2100 bioanalyzer
5. Mutation detection for the K- rasand P16 genes
6. Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager
7. The combination of monolithic columns and modern HPLC/MS/MS instrumentation for high chromatographic throughput and sensitive detection of beta-agonists
8. TKXtra DataFile: Homogenous tyrosine kinase detection using High Efficiency Fluorescence Polarization
9. cAMP DataFile: Homogenous Cyclic AMP detection using High Efficiency Fluorescence Polarization
10. Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis
11. Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.
Post Your Comments:

(Date:10/12/2015)... This report covers the global cell expansion ... applications, end-user markets and geographic segmentation. Forecasts are provided ... cell expansion market generated revenue of roughly $8.5 billion ... $9.7 billion in 2015 and $22.0 billion by 2020, ... from 2015 to 2020. This report provides: ...
(Date:10/12/2015)... Oct. 12, 2015  Patara Pharma, a clinical-stage ... inflammatory diseases and conditions, today announced the closing ... financing. Concurrent with the close of its sale ... Loan and Security Agreement with Silicon Valley Bank ... million. Patara will use the funds from the ...
(Date:10/12/2015)... ... October 12, 2015 , ... NeuMedics Inc., is a specialty biopharmaceutical company focused ... safely and chronically be administered as an eye drop, announced today it has been ... Clinic and taking place October 25th to October 28th at The Cleveland Clinic, Cleveland, ...
(Date:10/9/2015)... ANNAPOLIS, Md. , Oct. 9, 2015 ... entered into the Congressional Record her statement recognizing the ... celebrated October 11-17. IPAW is sponsored by the ... companies, and is designed to:   , Raise ... contributions of plasma donors in saving and improving lives ...
Breaking Biology Technology:
(Date:9/30/2015)... , Sept. 30, 2015  With nearly 300,000 Americans ... of new SCIs estimated to reach 12,500 annually, the ... California Resource Services for Independent Living (SCRS-IL) is ... California opening doors to independence ... programs and services, notably assistive technology services and education. ...
(Date:9/29/2015)... 29, 2015 News facts: ... also saving energy , Minimized design shrinks PC ... Active Mode and embedded Fujitsu PalmSecure authentication enable enterprises ... Fujitsu today shows that good things come in small ... to its enterprise desktop and mobile portfolio. Featuring workplace ...
(Date:9/28/2015)... YORK , Sept. 28, 2015  The ... respiration rate and body temperature, is an essential ... can be indicators of deterioration in a patient,s ... hospital, vital signs are typically taken during routine ... hours. If a patient deteriorates between these observation ...
Breaking Biology News(10 mins):