( When working with highly-concentrat...)Front-face detection for high-concentrate, opaque or solid sample

When working with highly-concentrated, opaque, or solid samples such as blood, paint, optical brighteners, living cells, polymer films, or phosphors, front-face fluorescence detection offers significant advantages for quantitative and qualitative fluorescence measurements. Highly concentrated and opaque liquids typically have problems with self-absorption and complete attenuation of the beam. When measuring fluorescence at 90, intensity measurements may not be reproducible or detectable, and the excitation or emission spectra may appear distorted. Front-face detection offers the solution for these problems.

Using the technique of front-face detection for opaque liquids and solids can be an important analytical tool for characterizing fluorescence of various sample types. In the front-face technique, the excitation light is focused to the front surface of the samples and then fluorescence emission is collected from the same region at an angle that minimizes reflected and scattered light.

Types of Samples
Highly concentrated: blood, oil, optical brighteners
Highly opaque: paints, phosphors
Solids: polymers, films, paper, contact lenses, crystals

Front-Face Detection
FLUOROLOG Use the 22.5 collection path for liquid and solid samples.
FLUOROMAX Use the Model 1933 Solid-Sample Holder at a 30/60 angle for solids. For liquids, use the Model 1967 Front-Face Thermostatted Cell Holder with magnetic stirrer accessory.

back to top
'"/>

Source:

Page: All 1
Related biology technology :

1. Monitoring transfection efficiency by green fluorescent protein (GFP) detection with the Agilent 2100 bioanalyzer
2. Apoptosis detection by annexin V and active caspase-3 with the Agilent 2100 bioanalyzer
3. Sensitive detection of tumor cells in peripheral blood of carcinoma patients by a reverse transcription PCR method
4. Rapid detection of genomic duplications and deletions using the Agilent 2100 bioanalyzer
5. Mutation detection for the K- rasand P16 genes
6. Multiplex protein detection with the ECL Plex fluorescent Western blotting system using the Ettan DIGE Imager
7. The combination of monolithic columns and modern HPLC/MS/MS instrumentation for high chromatographic throughput and sensitive detection of beta-agonists
8. TKXtra DataFile: Homogenous tyrosine kinase detection using High Efficiency Fluorescence Polarization
9. cAMP DataFile: Homogenous Cyclic AMP detection using High Efficiency Fluorescence Polarization
10. Using the Agilent 2100 bioanalyzer for quality control of protein samples prior to MS-analysis
11. Quality assurance of RNA derived from laser microdissected tissue samples obtained by the PALM MicroBeam System using the RNA 6000 Pico LabChip kit.