Navigation Links
Fractionation of Bovicola ovis Homogenates Using the Mini Whole Gel Eluter

Brian J. Shiell and Wojtek P. Michalski, CSIRO Division of Animal Health, Australian Animal Health Laboratory, Geelong, Victoria, Australia


Introduction

Amino acid sequence and composition analyses of proteins can be performed on samples derived from polyacrylamide gels. Protein electro-elution, the most commonly used method of recovering proteins from gels, can be performed on cut-out gel slices or using the Whole Gel Eluter recently introduced by Bio-Rad.14 The Mini Whole Gel Eluter was used here to fractionate crude homogenate of the common sheep louse with the aim of characterizing immunogenic components.


Methods

Common sheep lice, Bovicola ovis, were harvested from infested animals, homogenized by ultrasonication, and the homogenate was clarified by centrifugation on a benchtop microfuge. Homogenate samples (1 mg protein) were separated by SDS-PAGE in 12% preparative well Tris-HCl Ready Gels (Bio-Rad). Protein fractions were immediately electro-eluted at 100 mA for 25 min using the Mini-Whole Gel Eluter. Three elution buffers: (A) 100 mM NH4HCO3, 0.01% SDS, pH 8.5, (B) 10 mM phosphate buffer, 0.005% SDS, pH 7.8, and (C) 25 mM Tris, 19.2 mM glycine, 0.01% SDS, pH 8.3, were used to determine the elution efficacy. The fractions were analyzed in 12%, 15 well Tris-HCl Ready Gels (Bio-Rad) and proteins were visualized by Coomassie blue staining. Bio-Rads low molecular weight standards for SDS-PAGE are shown in Figure 1B, lane b.


Results and Discussion

Louse homogenate preparations are often rich in lipids and do not separate well on SDS-PAGE gels (
'"/>

Source:


Page: All 1 2 3

Related biology technology :

1. Efficient Cellular Fractionation for RNA and Protein Isolation
2. Sample Preparation Solutions for Cellular Protein Fractionation Poster, Rev A
3. Low Abundance cDNA Cloned Using Stratagenes Human Universal cDNA Library
4. Oil-Free PCR Using the Hot Top Assembly
5. Functional Cloning Using ViraPort Retroviral cDNA Expression Libraries
6. Signal Transduction Reporting Systems Using Cis-Acting Enhancer Elements
7. Using XL1-Red Mutator Strain to Generate Esterase Variants
8. Detect Released Oligosaccharides Using the Eagle Eye II Still Video Imaging System
9. Comparison of Growth Techniques and Media for the Purpose of Plasmid Isolation from E. coli Using the Eppendorf Perfectprep Plasmid Mini Kit
10. Genomic DNA Extraction from Buccal Swabs Using the Perfect gDNA Blood Mini Kit
11. Genomic DNA Extraction from Buffy Coat Using the Perfect gDNA Blood Mini Kit
Post Your Comments:
(Date:9/17/2014)... The global consumption of squalene / squalane is ... natural skin-identical chemical that finds applications in various end user ... and in other applications like high grade lubrication, and fiber ... major players and is regionally segregated. Sophim ( France ... Ltd. ( Japan ), SeaDragon Marine Oils Ltd. ...
(Date:9/17/2014)... Md. (PRWEB) September 17, 2014 ... Comprehensive Two-Dimensional Liquid Chromatograph (2D-LC), which offers ... complex sample matrices. The system provides a ... sample analytes, which is beneficial to scientists ... extracts, and natural products. , Comprehensive 2D-LC ...
(Date:9/17/2014)... Pa. , Sept. 17, 2014 BioClinica®, ... and eClinical Solutions, today announced three of its experts ... for Clinical Data Management to be held September ... Nevada . The Company also announced the innovative ... raise overall quality in clinical trials acquired earlier this ...
(Date:9/17/2014)... Sept. 17, 2014 Hussey Copper , ... 29 antimicrobial copper, announces Gilmour Academy Ice Arena,s ... first EPA-registered antimicrobial solid touch surface to help protect ... reasonably priced surface that kills greater than 99.9% of ... exposure. 1 , ...
Breaking Biology Technology:Squalene Market by Source (Animal & Vegetable) & by End-user Industry (Cosmetics, Pharmaceuticals & Food Industry) - Global Trends & Forecasts to 2019 2Squalene Market by Source (Animal & Vegetable) & by End-user Industry (Cosmetics, Pharmaceuticals & Food Industry) - Global Trends & Forecasts to 2019 3Shimadzu’s New Nexera-e Two Dimensional Comprehensive Liquid Chromatograph Empowers Laboratories to Separate Even the Most Complex Mixtures 2BioClinica to Present at 20th Annual SCDM Conference 2BioClinica to Present at 20th Annual SCDM Conference 3Innovative Gilmour Academy Ice Arena changes more than 200 fixtures to Hussey Copper's MD-Cu29 antimicrobial copper to help protect against bacteria 2Innovative Gilmour Academy Ice Arena changes more than 200 fixtures to Hussey Copper's MD-Cu29 antimicrobial copper to help protect against bacteria 3
... Spherix Incorporated (NASDAQ: SPEX ) – ... syndrome and atherosclerosis, and provider of technical and regulatory ... today announced that it has entered into definitive agreements ... stock and warrants to purchase shares of its common ...
... +49(0)69-2222-34066 France: +33(0)1-70-48-01-63 U.K.: +44(0)20-7660-0009 ... is 5634610; to view the webcast, log onto: ... March 13, 2012 - Annual press conference in Goettingen, Germany ... Germany April 24, 2012 - Publication of first-quarter ...
... A relatively fast, easy and inexpensive technique for ... self-assemble into one-, two- and even three-dimensional macroscopic ... researchers with the U.S. Department of Energy (DOE),s ... should enable more effective use of nanorods in ...
Cached Biology Technology:Spherix Announces Registered Direct Offering of $1.15 Million 2Spherix Announces Registered Direct Offering of $1.15 Million 3Preliminary Figures for 2011: Sartorius Grows at Double-Digit Rates and Boosts Operating Earnings by More Than 30% 2Self-assembling nanorods: Berkeley Lab researchers obtain 1-, 2- and 3-D nanorod arrays and networks 2Self-assembling nanorods: Berkeley Lab researchers obtain 1-, 2- and 3-D nanorod arrays and networks 3Self-assembling nanorods: Berkeley Lab researchers obtain 1-, 2- and 3-D nanorod arrays and networks 4
(Date:9/17/2014)... and their environmental impact recently scored a major win. ... on the bags, and Governor Jerry Brown is expected ... not yielding without a fight, according to an article ... newsmagazine of the American Chemical Society. , Alexander Tullo, ... campaign logged its first small victory in 1990 when ...
(Date:9/17/2014)... at IMIM (Hospital del Mar Medical Research Institute) and ... published a study in eLife showing that ... the evolution of new proteins, some of which could ... produce proteins from the instructions found in an RNA ... RNA containing information for the synthesis of proteins, meaning ...
(Date:9/17/2014)... the Catalan Institute of Oncology-Bellvitge Biomedical Research Institute ... genes differentially expressed in normal accompanying cells in ... used to classify colorectal tumors, predict the evolution ... decisions to prevent relapses. , Biomarkers ... in our population considering both sexes. About 30,000 ...
Breaking Biology News(10 mins):Parts of genome without a known function may play a key role in the birth of new proteins 2Five genes to predict colorectal cancer relapses 2
... outbreak of avian or "bird flu" lingers, health officials recognize ... the virus already have developed resistance to the current roster ... Diego scientists - with the help of resources at the ... - have isolated more than two dozen promising and novel ...
... University have found that quantum dot nanoparticles can penetrate ... into potential workplace concerns for healthcare workers or individuals ... research on potential biomedical applications of the tiny nanoparticles. ... different sizes, shapes and surface coatings do not penetrate ...
... German . , Metabolomics aims to determine the totality ... The exponents of bioinformatics analyzed data collected in the framework ... In each case, a subgroup of the animals was treated ... were quantitatively determined in a blood plasma sample of a ...
Cached Biology News:UC San Diego researchers identify potential new drug candidates to combat 'bird flu' 2UC San Diego researchers identify potential new drug candidates to combat 'bird flu' 3UC San Diego researchers identify potential new drug candidates to combat 'bird flu' 4Study shows quantum dots can penetrate skin through minor abrasions 2Healthy or diseased? 2
MDMA Mouse Monoclonal Antibody (Clone 3G8)...
Mouse anti-hepatitis delta virus Class: Antibody Product Group: Bacterial and Viral Antigen...
Mouse monoclonal [MN-8C4] to zona radiata ( Abpromise for all tested applications). Antigen: Purified eggshell proteins (zona radiata proteins and small amounts of zona pellucida proteins) from ...
Goat polyclonal to PPP2R5D Immunogen: Peptide with sequence KRAEEFLTASQEAL, from C Terminus of the protein sequence according to NP_006236...
Biology Products: