Navigation Links
Fractionating DNA Fragments Generated by Differential Display PCR

CastAway system application

Iain Kilty Phil Vickers
Pfizer Central Research, Sandwich, UK. 01304 618482

Differential display PCR (DDPCR) is a widely used technique for identifying genes differentially expressed between cell types. Application of DDPCR to allergic disease provides a potential means for elucidating the mechanisms involved in allergic responses. In order to successfully apply DDPCR, high-quality, reproducible denaturing polyacrylamide gels are required to fractionate PCR fragments. Stratagenes CastAway system* is convenient and consistently yields the high-quality band resolution that is necessary for DDPCR.

Allergic disease represents a major public health problem in most countries, affecting people of all races. Over 100 million people worldwide suffer from asthma alone.1 Allergic disease is treated predominantly with the use of steroids. Although steroids are effective in eliminating the symptoms of allergic disease, they also cause a number of detrimental side effects. Thus, a greater understanding of the genes involved in the pathogenesis of allergic disease is needed to allow more acceptable drugs to be developed.

As leukocytes play a key role in mediating allergic responses, a comparison of the expression levels of genes in the leukocytes of people suffering from allergic disease as compared to nonallergic controls may provide insights into the basis of such disease. A number of methods may be used for identifying differentially expressed genes, such as high-throughput sequencing of expressed sequence tags (ESTs), subtractive hybridization of cDNA and DDPCR. DDPCR is often the method of choice for identifying differentially expressed genes because it (1) requires relatively little starting material, (2) can be used to screen a large proportion of the mRNA population and (3) does not require the sequencing of large number
'"/>

Source:


Page: All 1 2 3 4

Related biology technology :

1. Mammalian Expression Vector for Efficient Cloning of PCR Fragments
2. Efficient Cloning and Electro-transformation of Large Eukaryotic DNA Fragments
3. Resolution of Linear DNA Fragments From 23 Kilobases to 6 Megabases Using Biphasic Linear Switch-Time Ramping
4. Inducing RNAi with siRNA Cocktails Generated by RNase III
5. Identification of Differentially Expressed Gene Products with the CastAway System*
6. Differential Gene Expression Analysis of Pure Cell Populations from Frozen UterineTissue Using Laser Capture Microdissection (LCM) and cDNA Microarrays
Post Your Comments:
(Date:5/16/2008)... /- Alba Therapeutics Corporation,ann...ts first patient in a,twelve-week Phase IIb trial ...stinal barrier dysfunction, for the treatment of p...l multi-center, randomized, double-blind,placebo-...stological,efficacy, safety and tolerability of la...
(Date:5/16/2008)... /- Stereotaxis, Inc. (Nasd...ef Executive Officer, will,present at the Seventh ...rancisco on Tuesday, May 20, 2008 at 12:00 p.m. Pa...nvitation only. The audio,presentation will be web...p://www.wsw.com/webcast/jmp6/stxs/ ., About Stere...
(Date:5/15/2008)...m Product Sales Increased 149% to $3.5 Million - ...-, TUSTIN, Calif., May 15 /PRNewswire-FirstCall/ ...ated specialty pharmaceutical company headquartere...en, Jiangxi and Jilin,China. Through its wholly ow... Company engages in the research, development, man...
(Date:5/15/2008)...Toronto Stock Exchange Symbol: MS, EDMONTON, May ...SX:,MS), a leading developer in the treatment of m...and operational results for the first quarter ende...illy and Company, is,developing dirucotide (also k... treatment of MS undergoing pivotal trials in Cana...
Breaking Biology Technology:Alba Therapeutics Announces the Enrollment and Randomization of the First Patient in the Phase IIb Clinical Trial for Larazotide Acetate (AT-1001) for the Treatment of Active Celiac Disease 2Alba Therapeutics Announces the Enrollment and Randomization of the First Patient in the Phase IIb Clinical Trial for Larazotide Acetate (AT-1001) for the Treatment of Active Celiac Disease 3AMDL Announces First Quarter 2008 Results Posting Sales of $3.58 Million 2AMDL Announces First Quarter 2008 Results Posting Sales of $3.58 Million 3BioMS Medical announces first quarter 2008 results 2BioMS Medical announces first quarter 2008 results 3BioMS Medical announces first quarter 2008 results 4BioMS Medical announces first quarter 2008 results 5
Editor,s Note: During July 2003, two British education officals visited Madison to determine whether the "Wisconsin Idea" can be exported. At a time when many Wisconsin leaders are studying how to
...Increases Attention of Weather Forecasting ,MADIS... hit the East Coast, the accuracy of weather forec...homes and businesses. People are watching TV, list...out the latest path of the storm. A new product fr...er of weather forecasting technology and Internet ...
...like some fries with your Internet connection? , ...gan installing wireless fidelity (Wi-Fi) Internet ... a six-month pilot program. These Milwaukee locati...Francisco Bay Area, Metropolitan New York City, Gr...onald,s Wireless Internet service. , ,Customers ca...
...sed. Governor Doyles new Growth Council seems like...heyre out of touch. After looking at the roster of... women were mentioned, to say nothing of minoritie...ing mission and found that: , ,1) Four out of five...Gen Xers (b. 1961-1981), and minorities (Source: U...
Other Biology Technology:Hurricane Ups Weather Forecasting Attention For Madison Firm 2Wisconsin McDonalds Go Wireless 2
(Date:5/15/2008)...LA JOLLA, CA - Now that high school biology studen... transcribed into messenger RNA (mRNA), which is t...ss of molecules, sending studentsand many scientis... Salk Institute for Biological Studies investigato...8 online issue of Developmental Cell, shows that t...
(Date:5/15/2008)...Fishermen hold empirical knowledge that tuna aggre...d rope, pieces of wood, or even large marine mamma...gregation behaviour, but the past 20 years have se... the associated concentrations of fish. Fishermen ...act as FADs. An enormous purse-seine net, deployed...
(Date:5/14/2008)...PHILADELPHIA Genetic differences may explain the ...en compared with Hispanic men, which could help cl... disease, according to a paper published in the Ma...ournal of the American Association for Cancer Rese...sessing risk for prostate cancer is the prostate s...
(Date:5/14/2008)...ANN ARBOR, Mich.---Researchers have developed what... 20 years for inhibiting a common target used to t... to a new class of AIDS drugs. , Researchers at ... develop the inhibiting compound, and then confirm...t HIV protease, which is an established target for...
Breaking Biology News(10 mins):Plant biologists discover unexpected proteins affecting small RNAs 2Plant biologists discover unexpected proteins affecting small RNAs 3Does fishing on drifting fish aggregation devices endanger the survival of tropical tuna? 2Does fishing on drifting fish aggregation devices endanger the survival of tropical tuna? 3Genes may play role in risk assessment for prostate cancer among Hispanics and caucasians 2Compound has potential for new class of AIDS drugs 2Merrill Lynch Global Pharmaceutical Biotechnology 26 Medical Device Conference to Webcast Alexzas Corporate Presentation 2797 1Merrill Lynch Global Pharmaceutical Biotechnology 26 Medical Device Conference to Webcast Alexzas Corporate Presentation 2797 2First of Its Kind National Survey Uncovers Obstacles to Comprehensive Healthcare for People With Schizophrenia 10309 1First of Its Kind National Survey Uncovers Obstacles to Comprehensive Healthcare for People With Schizophrenia 10309 2First of Its Kind National Survey Uncovers Obstacles to Comprehensive Healthcare for People With Schizophrenia 10309 3First of Its Kind National Survey Uncovers Obstacles to Comprehensive Healthcare for People With Schizophrenia 10309 4Official Ground Breaking Ceremony for Cleveland Clinic Abu Dhabi 10306 1Official Ground Breaking Ceremony for Cleveland Clinic Abu Dhabi 10306 2Official Ground Breaking Ceremony for Cleveland Clinic Abu Dhabi 10306 3Official Ground Breaking Ceremony for Cleveland Clinic Abu Dhabi 10306 4Digital mammography superior to film mammography in some cases 10303 1Digital mammography superior to film mammography in some cases 10303 2
...placed a single atom from the molecular structure ...hat attacks the bacteria by inhibiting cell wall s...trum of activity. In recent years, a number of the...sistant to vancomycin and use of the antibiotic ha...could help make the drug more effective in treatin...
...ough for healthy people, but the disease can place...stems, such as patients on chemotherapy. A five-ye...Hospital of Philadelphia will support research to ...mune systems may be uniquely vulnerable to influen...Because patients with compromised immune systems m...
...for implants that may improve construction or repa...eveloped by researchers from the American Dental A...itute of Standards and Technology (NIST). , Descr... new technology provides a method for making scaff...a patient,s own cells and is formed with a cement ...
...antation is among one of the most exciting and hot... the promise of personalized medicine and effectiv...ess in this area, moral and ethical dilemmas about... research presented today at the American College ...ientists continue to explore mature stem cell sour...
Other Biology News:Scientists re-engineer a well-known antibiotic to counter drug resistance 2How can we protect patients with weakened immune systems from influenza? 2How can we protect patients with weakened immune systems from influenza? 3Bioactive cement scaffold may improve bone grafts 2Potential heart benefit found in stem cells 2Potential heart benefit found in stem cells 3Potential heart benefit found in stem cells 4Potential heart benefit found in stem cells 5
T4 Polynucleotide Kinase catalyzes the transfer of the ?-phosphate from ATP to the 5-terminus of polynucleotides or to mononucleotides bearing a 5-hydroxyl group. The enzyme, purified from recombinan
Thermo Labsystems Nepheloskan Ascent is the first nephelometer ever adapted to measurement in microplates. Nepheloskan measures light scattering and offers an ultra-sensitive technique for particle q
Anti-VDAC (Voltage-Dependent Anion Channel) AVOID FREEZE/THAW CYCLES. Recognizes the porin 31HL protein. Corresponds to mAb 1 in the literature.
6 x Blend of rTth DNA Polymerase and Tli to provide optimized amounts of both 5-3 and 3-5 proofreading activity
Biology Products: