( Introduction ...)FluoroMax multiwavelength capability

Introduction
Simultaneously monitoring the levels of relevant metabolites and cell parameters is necessary to properly assess the effectiveness of drugs on living cells. The FLUOROMAX spectrofluorometer allows the user to monitor up to eight wavelength-pairs of emission and excitation wavelengths. Coupling this capability with the proper probes can produce a real-time analysis of unfolding biological processes.

Results
One research application that used simultaneous observation of multiple wavelength-pairs was performed by M. Kimura, et al., at the University of Medicine and Dentistry of New Jersey. ¹ Their evaluation of Thapsigargin, a hypertensive drug, was performed on a FLUOROMAX spectrofluorometer. The results demonstrated that the effect of the drug in human platelets was not limited to the elevation of the Ca²⁺ but produced substantial changes in the overall ionic balance.

Figure 1 shows a sample screen display taken from DATAMAX (FLUOROMAX software for Windows). Note how DATAMAX makes the monitoring of up to eight wavelengthpairs a simple matter of selection.

Conclusion
FLUOROMAX, with DATAMAX software, allows the user to monitor wavelengthpairs to characterize processes in living cells and other biological systems. The measurement is feasible in real time because of the FLUOROMAX spectrofluorometers ability to scan rapidly (up to 200 nm s¹).

¹ Kimura, M.; et al. J. Physiol. 1993, 494, 113.

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