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Fluorescently Labeling Long dsRNA with the Silencer siRNA Labeling Kit

labeled dsRNA was passed on to daughter cells during cell division (data not shown).

Figure 1. Fluorescently Labeled dsRNA Retains Functionality. The MEGAscript RNAi Kit was used to generate full-length dsRNA to the Drosophila hrp48 and U2AF50 genes from the corresponding cDNA constructs. A portion of the dsRNA was labeled with Cy3 using the Silencer siRNA Labeling Kit according to the above protocol. 1x106 Schneider's Drosophila L2 cells were grown in 6 well plates in serum free medium and directly treated with 10 nM of dsRNA (no transfection is required in these cells). Three days after treatment, (A) hrp48 and U2AF50 protein levels were assessed by Western blot and (B) cells were analyzed by fluorescence microscopy. BLUE: DAPI stained nuclei. RED: Cy3 labeled U2AF50 dsRNA.


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Cat# Product Name Size 1626 MEGAscript RNAi Kit 20 rxns 1632 Silencer siRNA Labeling Kit - Cy3
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