Navigation Links
Fluorescence assay for determination of protease activity

In research and industrial work it is quite often necessary to test whether samples or solutions are free of protease activity. Using commercially available kits e.g. from Molecular Probes Inc., protease activity can be measured with the new fluorometer from PerkinElmer Life Sciences, VICTOR.

VICTOR is a new multitechnology and multilabel counter from Wallac that can measure fluorescent, photometric and luminescent labels as well as DELFIA research assays in microtitration plates. It is an epifluorometer that allows the use of both clear and coloured plates depending on the assay requirements.


In the reaction protease digests a highly quenched BODIPY FL-casein substrate releasing fluorescent peptides. The trypsin used as standard was diluted in 10 mmol/l Tris-HCl buffer, pH 7.8 of which 100 μl was added to the well.

After addition of 100 μl substrate, 10 μg/ml, diluted in 10 mmol/l Tris HCl buffer, pH 7.8, the plate was incubated overnight at room temperature and then measured.


The detection was done using the factory preset settings for fluorescein label. Excitation was at 485 nm and emission was measured at 535 nm.


Measurement with the new VICTOR fluorometer gives results comparable to the data given by the kit manufacturer.


Fast and simple sample preparation.


1420-011 VICTOR2 multilabel counter

1420-012 VICTOR2 multilabel counter with TR-fluorescence option

1420-014 VICTOR2 basic with stacker and robotic loading system

1420-015 VICTOR2 with TRF with stacker and robotic loading sy stem

1420-041 VICTOR2V with stacker


Trypsin enzyme from bovine pancreas, activity 10700 units/mg (Sigma T-8253)

EnzCheck Protease Assay kit from Molecular Probes Inc. (E-6638) Labsystems black Combiplate ( 95029490)


Insert of the EnzCheck Protease Assay Kit from Molecular Probes Inc. (E-6638).



Page: All 1 2

Related biology technology :

1. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
2. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
3. Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect
4. Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000
5. Minimizing Photobleaching of Blue Fluorescent Protein (BFP) using the Varian Cary Eclipse Fluorescence Spectrophotometer
6. Receptor Binding Assays using Fluorescence Polarization Detection: the [FP]2TM Characterized Tracers
7. TKXtra DataFile: Homogenous tyrosine kinase detection using High Efficiency Fluorescence Polarization
8. cAMP DataFile: Homogenous Cyclic AMP detection using High Efficiency Fluorescence Polarization
9. LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)
10. FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses
11. Enzyme Immunoassay for Studying Intracellular Levels of cAMP
Post Your Comments:

(Date:10/12/2015)... Oct. 12, 2015 /PRNewswire/ - Aeterna Zentaris Inc. (NASDAQ: ... engaged in developing and commercializing novel treatments in oncology, ... Dennis Turpin , the Company,s former Senior Vice ... its Quebec City office.  ... Chief Executive Officer of the Company commented, "After a ...
(Date:10/12/2015)... October 12, 2015 LabStyle Innovations ... Management Solution, today announced its Medical Director, Dr. ... at MobiHealth,s 5th EAI International Conference on Wireless ... through innovations in mobile and wireless technologies," the conference ... from October 14 - 16, 2015. The conference ...
(Date:10/12/2015)... , Oct. 12, 2015 VolitionRx Limited ... a completed clinical study of its NuQ ® blood-based ... the online issue of Clinical Epigenetics , the official ... conducted in collaboration with Lund University, ... Andersson , MD, PhD, Professor of Surgery and Vice-Dean, Faculty ...
(Date:10/12/2015)... Seattle WA (PRWEB) , ... October 12, 2015 , ... ... for the treatment of retinal diseases that can safely and chronically be administered as ... Global Health Impact Forum co-hosted by The Cleveland Clinic and taking place October 25th ...
Breaking Biology Technology:
(Date:10/6/2015)... , Oct. 6, 2015 Track Group, ... today that it has signed a contract with the ... across the full range of sentences under the Department,s ... of the Americas. "This contract with the Virginia DOC ... the US and advances our position as a trusted ...
(Date:10/5/2015)... ) releases ... (NASDAQ: NXTD ), a biometric authentication company focused ... ) releases the following market and ... a biometric authentication company focused on the growing mobile ... ) releases the following market and ...
(Date:10/1/2015)... Oct. 1, 2015  Biometrics includes diverse set ... body characteristics, such as fingerprints, eye retinas, facial ... of biometrics technology has been constantly increasing in ... five years. In addition to the most prominent ... recognition, other means of biometric authentication are rapidly ...
Breaking Biology News(10 mins):