Navigation Links
Fluorescence assay for determination of protease activity

In research and industrial work it is quite often necessary to test whether samples or solutions are free of protease activity. Using commercially available kits e.g. from Molecular Probes Inc., protease activity can be measured with the new fluorometer from PerkinElmer Life Sciences, VICTOR.

VICTOR is a new multitechnology and multilabel counter from Wallac that can measure fluorescent, photometric and luminescent labels as well as DELFIA research assays in microtitration plates. It is an epifluorometer that allows the use of both clear and coloured plates depending on the assay requirements.

DESCRIPTION OF ASSAY

In the reaction protease digests a highly quenched BODIPY FL-casein substrate releasing fluorescent peptides. The trypsin used as standard was diluted in 10 mmol/l Tris-HCl buffer, pH 7.8 of which 100 μl was added to the well.

After addition of 100 μl substrate, 10 μg/ml, diluted in 10 mmol/l Tris HCl buffer, pH 7.8, the plate was incubated overnight at room temperature and then measured.

DETECTION

The detection was done using the factory preset settings for fluorescein label. Excitation was at 485 nm and emission was measured at 535 nm.

RESULTS

Measurement with the new VICTOR fluorometer gives results comparable to the data given by the kit manufacturer.

BENEFITS

Fast and simple sample preparation.

PRODUCTS AVAILABLE

1420-011 VICTOR2 multilabel counter

1420-012 VICTOR2 multilabel counter with TR-fluorescence option

1420-014 VICTOR2 basic with stacker and robotic loading system

1420-015 VICTOR2 with TRF with stacker and robotic loading system

1420-041 VICTOR2V with stacker

OTHER PRODUCTS NEEDED

Trypsin enzyme from bovine pancreas, activity 10700 units/mg (Sigma T-8253)

EnzCheck Protease Assay kit from Molecular Probes Inc. (E-6638) Labsystems black Combiplate (cat.no. 95029490)

REFERENCES

Insert of the EnzCheck Protease Assay Kit from Molecular Probes Inc. (E-6638).


'"/>

Source:


Page: All 1 2

Related biology technology :

1. New Mammalian Expression Vectors Employ Stable, High-Level Fluorescence Humanized Renilla GFP Reporter
2. Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations
3. Visualizing siRNA in Mammalian Cells: Fluorescence Analysis of the RNAi Effect
4. Identification of cells in S phase using the Cell Proliferation Fluorescence Kit and IN Cell Analyzer 1000
5. Minimizing Photobleaching of Blue Fluorescent Protein (BFP) using the Varian Cary Eclipse Fluorescence Spectrophotometer
6. Receptor Binding Assays using Fluorescence Polarization Detection: the [FP]2TM Characterized Tracers
7. TKXtra DataFile: Homogenous tyrosine kinase detection using High Efficiency Fluorescence Polarization
8. cAMP DataFile: Homogenous Cyclic AMP detection using High Efficiency Fluorescence Polarization
9. LIVE/DEAD Viability/Cytotoxicity Assay for Animal Cells Using the SpectraMax Gemini XS Fluorescence Microplate Reader (MaxLine Application Note #43)
10. FLIPR Calcium 3 Assay Kit - An Optimal No-Wash Fluorescence Calcium Indicator Reagent for Non-Optimal Calcium Responses
11. Enzyme Immunoassay for Studying Intracellular Levels of cAMP
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Fluorescence assay for determination protease activity

(Date:7/13/2014)... -- The discovery 30 years ago of soccer-ball-shaped carbon ... nanotechnology research. Now, there appears to be a new ... Shanxi University and Tsinghua University in China have shown ... hollow molecular cage similar to a carbon buckyball. It,s ... only a matter of speculationdoes indeed exist. , "This ...
(Date:7/12/2014)... New York (PRWEB) July 12, 2014 ... analysis for various reagents used in the ... is driving the life science reagents market ... study include manufacturers and providers of life ... Reagents Market Report: http://www.transparencymarketresearch.com/life-sciences-reagents-analytical-reagents.html . , ...
(Date:7/11/2014)... , Not for release, publication or ... any jurisdiction where to do so would constitute a violation ... plc ("Shire" or the "Company") (LSE: SHP, NASDAQ: ... it has held a meeting with representatives of AbbVie. ... prior agreement or approval of AbbVie. A further ...
(Date:7/11/2014)... Research ( http://www.bccresearch.com ) reveals in its new report, ... MARKETS , the global market for digital polymerase chain ... This is estimated to grow to $490 billion in ... of 28.6%. , New digital PCR technology has created ... broader PCR field. The opportunities presented by this technology ...
Breaking Biology Technology:Researchers discover boron 'buckyball' 2Researchers discover boron 'buckyball' 3Transparency Market Research: Life Science Reagents Market- PCR Reagent Kits, Cell and Tissue Culture, Flow Cytometry, Electrophoresis and Chromatography 2Transparency Market Research: Life Science Reagents Market- PCR Reagent Kits, Cell and Tissue Culture, Flow Cytometry, Electrophoresis and Chromatography 3Shire plc - Statement re: Media Speculation 2Global Market for Digital Polymerase Chain Reaction Technology to Reach $490 Billion by 2018; Equipment Sector Accounts for the Fastest Growing Segment With 33% CAGR 2Global Market for Digital Polymerase Chain Reaction Technology to Reach $490 Billion by 2018; Equipment Sector Accounts for the Fastest Growing Segment With 33% CAGR 3
... blood, termed ,endometrial regenerative cells, (ERCs) are capable ... of advanced peripheral artery disease. A study published ... Translational Medicine demonstrates that when circulation-blocked mice were ... restored. , Critical limb ischemia, an ...
... SAN JOSE, Calif., Aug. 18 VNUS(R) Medical,Technologies, ... quarter results and,revises its full year 2008 guidance. ... operating expenses and certain balance sheet accounts,to record ... appropriately recorded in prior periods since the adoption ...
... (OTC Bulletin Board: ARNI), a clinical-stage biopharmaceutical company,focused on ... ended June 30, 2008., Net loss was $3.8 ... June 30, 2008, compared to $0.5 million, or $0.05 ... was $7.5 million, or $0.65 per share, for the,six ...
Cached Biology Technology:Limbs saved by menstrual blood stem cells 2VNUS Corrects Second Quarter 2008 Results and Revises Full Year 2008 Guidance 2VNUS Corrects Second Quarter 2008 Results and Revises Full Year 2008 Guidance 3VNUS Corrects Second Quarter 2008 Results and Revises Full Year 2008 Guidance 4Arno Therapeutics Announces Second Quarter 2008 Financial Results 2Arno Therapeutics Announces Second Quarter 2008 Financial Results 3Arno Therapeutics Announces Second Quarter 2008 Financial Results 4Arno Therapeutics Announces Second Quarter 2008 Financial Results 5
(Date:7/14/2014)... to the discovery and description of Rhipidocyrtus muiri ... turned out to represent a new genus and species. The ... from Borneo is discussed in a recent paper published in ... The holotype male, and only known specimen of Rhipidocyrtus ... subsequent to this it was transferred among several researchers in ...
(Date:7/14/2014)... consequence of aging? , Although it is widely thought ... risk of developing several common cancers decreases with age. ... decrease with age in the risk of developing certain ... puzzle was presented in a recent paper published in ... P. Brody of the Department of Biomedical Engineering at ...
(Date:7/13/2014)... A new study in women suggests that experiencing one ... single high-fat meal can slow the body,s metabolism, potentially ... about the previous day,s stressors before giving them a ... fat. The scientists then measured their metabolic rate ... and fat and took measures of blood sugar, ...
Breaking Biology News(10 mins):Serendipity at the Smithsonian: The 107-year journey of the beetle Rhipidocyrtus muiri 2Serendipity at the Smithsonian: The 107-year journey of the beetle Rhipidocyrtus muiri 3Weighty issue: Stress and high-fat meals combine to slow metabolism in women 2Weighty issue: Stress and high-fat meals combine to slow metabolism in women 3Weighty issue: Stress and high-fat meals combine to slow metabolism in women 4
... the most common childhood cancer is cured in about ... number was closer to five percent. In efforts to ... Children's Research Hospital, the University of Tennessee, and the ... play a role in the effectiveness of chemotherapy drugs. ...
... nanotubes make an ideal scaffold for the growth of bone ... broken bones, allowing them to simply inject a solution of ... appears in the June 14 issue of the American Chemical ... scientific society. , The success of a bone graft depends ...
... primordial soup that produced life on earth, there were ... acid chains, which were the first elements able to ... these molecules were ribonucleic acid (RNA) chains, a molecule ... has the secondary role in cells of copying information ...
Cached Biology News:Genes linked to treatment resistance in children with leukemia 2Nanotubes inspire new technique for healing broken bones 2Nanotubes inspire new technique for healing broken bones 3Life's origins were easier than was thought 2
Molecular Biological Resources offers bulk quantities of existing protein products. Please see the Chimerx divisions website for existing products and contact lloconne@molbiores.com for pricing....
... 9001:2000 Certified USDA Research Registered OLAW Assured ... Training AALAS Certified Technicians Documentation ... Documentation Upon Request Focus CRP ... all your custom immunology service needs. ...
... BalanceTalk XL provides balance data collection and ... Complete integration with Microsoft Excel , ... moisture analyzers , New balances can be added ... Collect data from multiple balances into one spreadsheet ...
Peptide-affinity Purified Polyclonal Antibody to Sphingolipid Receptor Edg8/S1P5...
Biology Products: