Navigation Links
Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations

se from the PCR/RFLP analysis. Third, 154 genotype-unknown human genomic DNA samples were genotyped by molecular beacon analysis for the three mutations, and results were confirmed for selected samples by DNA sequencing.

Conclusions

Use Stratagenes single-tube allelic discrimination assays to realize several advantages over existing mutation detection techniques: The hairpin-shaped molecular beacon probes are more specific in distinguishing single base-pair mismatches than linear probes.2 Because the test is performed in a closed tube and no post-PCR manipulation of samples is required, the test saves time and effort and significantly reduces the risk of PCR product carry-over contamination. Being able to use two allele-specific molecular beacons in the same PCR solution enables three possible genotype representations (two homozygotes and a heterozygote) of two allelic variants in target DNA to be determined at the same time. It also definitively discriminates a true negative result from a false negative result due to PCR failure. Finally, the technology can be adapted for high-throughput assays. Currently, with the 96-well PCR plate, it takes about 4 hours to completely screen 96 DNA samples; further enhancement of the assay throughput can be achieved by using a higher density plate format (e.g., 384-well format).

Acknowledgments

The authors thank the following: Dr. P.N. Ray (Molecular Diagnostic Laboratory, the Hospital for Sick Children, Toronto, Canada) for providing the CF genotype-specific human genomic DNA; Dr. J.W. Longshore (Molecular Diagnostic Laboratory, Greenwood Genetic Center, Greenwood, SC) and Dr. V.M. Pratt (LabCorp, Research Triangle Park, NC) for providing the factor II genotype-specific human genomic DNA; Dr. J.W. Longshore (Molecular Diagnostic Laboratory, Greenwood Genetic Center, Greenwood, SC) and Dr. C. Mura (Laboratoi
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
2. Using Validated siRNAs in Functional Genomic Assays
3. Nuclease Protection Assays: The Basics
4. Selection of Standards for Bio-Plex Cytokine Assays, Rev A
5. Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays
6. The Gradient Feature: Use in Optimization of Allelic Discrimination Assays, Rev B
7. The iCycler iQ Detection System for TaqMan Assays, Rev B
8. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma
9. Caspase-3 Activation - An Indicator of Apoptosis in Image-Based Assays
10. Amplification Efficiency of TaqMan Gene Expression Assays
11. Cell Proliferation Assays
Post Your Comments:
(Date:1/23/2015)... , Jan. 23, 2015  Array BioPharma Inc. (NASDAQ: ... reached a definitive agreement with Novartis Pharma AG to ... currently in Phase 3 development.  This agreement is conditional on ... PLC (GSK) on April 22, 2014, which are expected to ...
(Date:1/22/2015)... 2015  Transwestern | RBJ today announces the firm brokered a ... Shire a leading biopharmaceutical company, at Two Ledgemont Center in ... Robert Richards , president, and Brian Cohen , senior ... five-floor building at 95 Hayden Ave. Photo - ...
(Date:1/22/2015)... 2015 The laboratory information management systems ... a number of technological advancements due to factors such ... need to integrate healthcare systems, and increasing government support ... system integration. Key players in the market focus on ...
(Date:1/22/2015)... 22, 2015 zFlo Motion , a ... announce an exciting product from a new partner. STT-Systems ... over the past 15 years, with offerings in the optical-motion ... 3D IMU system (inertial measurement unit), iSen, is opening eyes ...
Breaking Biology Technology:Array Announces Agreement To Acquire ENCORAFENIB (LGX818) 2Array Announces Agreement To Acquire ENCORAFENIB (LGX818) 3Array Announces Agreement To Acquire ENCORAFENIB (LGX818) 4Array Announces Agreement To Acquire ENCORAFENIB (LGX818) 5Array Announces Agreement To Acquire ENCORAFENIB (LGX818) 6Array Announces Agreement To Acquire ENCORAFENIB (LGX818) 7Transwestern | RBJ Advises Shire in 202,000 SF Lease, Creating Boston's Largest Suburban Biotech Campus 2Laboratory Information Management Systems Market is Expected to Reach $1,323.6 Million by 2019 - New Research Report by MarketsandMarkets 2Laboratory Information Management Systems Market is Expected to Reach $1,323.6 Million by 2019 - New Research Report by MarketsandMarkets 3Laboratory Information Management Systems Market is Expected to Reach $1,323.6 Million by 2019 - New Research Report by MarketsandMarkets 43D Motion Capture Just Got Easy 2
... ... announced that its Vice President of eLearning and Informatics earned the ... Society for Training and Development (ASTD) Certification Institute. Michelle Flewell ... training experience and is responsible for the development of the company,s ...
... ... Human Anthrax Vaccine Program must be stopped. Scott Miller and Company ... one-hour television documentary "Fatal Immunity: The Human Anthrax Vaccine Story" to inform ... science turned into for-profit human anthrax vaccines with no accountability to its ...
... Nov. 4 Onyx Pharmaceuticals, Inc. (Nasdaq: ONXX ... Credit Suisse 18th Annual Healthcare Conference on Wednesday, November ... Time). Interested parties may access a live webcast ... http://www.onyx-pharm.com/view.cfm/32/Event-Calendar , It is recommended that listeners ...
Cached Biology Technology:BioSoteria eLearning Executive Michelle Nolin Flewell Earns Certification 2BioSoteria eLearning Executive Michelle Nolin Flewell Earns Certification 3BioSoteria eLearning Executive Michelle Nolin Flewell Earns Certification 4Scott Miller's 'A Call To Arms' And 'Fatal Immunity' Are Available For World-Wide Licensing 2Scott Miller's 'A Call To Arms' And 'Fatal Immunity' Are Available For World-Wide Licensing 3Scott Miller's 'A Call To Arms' And 'Fatal Immunity' Are Available For World-Wide Licensing 4Scott Miller's 'A Call To Arms' And 'Fatal Immunity' Are Available For World-Wide Licensing 5Scott Miller's 'A Call To Arms' And 'Fatal Immunity' Are Available For World-Wide Licensing 6Scott Miller's 'A Call To Arms' And 'Fatal Immunity' Are Available For World-Wide Licensing 7
(Date:1/22/2015)... man-made form of insulin delivered by nasal spray may ... with mild cognitive impairment and Alzheimer,s disease dementia, according ... Forest Baptist Medical Center. The study,s subjects ... (MCI) or mild to moderate Alzheimer,s dementia (AD). Those ...
(Date:1/22/2015)... POINT ROBERTS, Washington , January 20, 2015 ... technology and tech stocks, releases video from the CES 2015 ... security consultant Apollo Robbins . Apollo shows ... Wocket™ biometric smart wallet, a product of NXT-ID, Inc. (NASDAQ: ...
(Date:1/22/2015)... January 22, 2015 , ... The European Patent Office to present a video retrospective ... nominations to be featured: Christofer Toumazou and Sophie ... finalists and winners of the Award   Starting on ...
Breaking Biology News(10 mins):Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 2Nasal Spray with Insulin Equivalent Shows Promise as Treatment for Adults with Mild Cognitive Impairment, Alzheimer's Dementia 3Apollo Robbins Talks about the Illusion of Personal Safety and How Easily Your Wallet is Stolen; If a Wocket Wallet is Stolen the Thief Can't do Anything With it! 2Apollo Robbins Talks about the Illusion of Personal Safety and How Easily Your Wallet is Stolen; If a Wocket Wallet is Stolen the Thief Can't do Anything With it! 3Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 2Ten Years of the European Inventor Award: A Retrospective Look at the Inventors and Ideas That Have Changed Our Lives 3
... scientist who has made ground-breaking discoveries in human genetics has ... in the United States of America. Professor Patricia ... in a human, specifically the 47,XXY constitution in males with ... an extra chromosome, causing symptoms such as infertility and tall ...
... scientist may have found a way to cut the amount of ... ingredient of the brewer,s art: hops. Cattle, deer, sheep, ... occurring bacteria to aid digestion of grass and other fibrous plants ... rumen. The problem, according to ARS microbiologist Michael Flythe, comes ...
... corresponding to 730 regions on the human genome by ... genome that were not charted in the reference map ... of those sequences are either missing, fragmented or misaligned ... on the same samples," said Dr. Evan Eichler, senior ...
Cached Biology News:National Academy of Sciences recognizes Southampton genetics scientist 2New methods identify thousands of new DNA sequences missing from the human genome reference map 2New methods identify thousands of new DNA sequences missing from the human genome reference map 3