Navigation Links
Fluorescence-Based Single-Tube Assays to Rapidly Detect Human Gene Mutations

as the quencher on both molecular beacons.

Fig.2

The assay is performed in a single PCR tube that contains a DNA template, both allele-specific molecular beacons and other PCR reagents. We carried out the amplification in the ABI Prism 7700 sequence detector. The genotype of a DNA sample is determined by measuring the endpoint fluorescence value (endpoint analysis, Figure 2) and/or the threshold cycle (Ct) value (real-time analysis) of the sample. Monitoring PCR reactions in real time produces a fluorescent signal during each cycle. Molecular beacon fluorescence is reported during each annealing step when the molecular beacon is bound to its complementary target (Figure 3 and Figure 4).

Results are displayed as an amplification plot that reflects the change in fluorescence during cycling. The threshold cycle is the cycle at which the fluorescent signal is first detectable above background. For the three assays described in the article, a Ct value of 20 to 32 indicates the presence of a specific allele and a Ct value of 40 indicates the absence of a specific allele. The maximum Ct value is 40 as PCR proceeds to 40 cycles.

The Specific Kits

The CFTR (DF508) Mx4000 molecular beacon allelic discrimination kitff, is designed to rapidly detect/screen the most common mutation that causes cystic fibrosis (CF). This mutation, a three-base-pair deletion, within exon 10 of the cystic fibrosis transmembrane conductance regulator (CFTR) gene, accounts for approximately 70% of all mutant CF alleles.3,4 The Factor II (G20210A) Mx4000 molecular beacon allelic discrimination kitff, is used to detect a common genetic variant in the co
'"/>

Source:


Page: All 1 2 3 4 5 6

Related biology technology :

1. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
2. Using Validated siRNAs in Functional Genomic Assays
3. Nuclease Protection Assays: The Basics
4. Selection of Standards for Bio-Plex Cytokine Assays, Rev A
5. Feasibility of Multiplexing Bio-Plex Total Target and Phosphoprotein Assays
6. The Gradient Feature: Use in Optimization of Allelic Discrimination Assays, Rev B
7. The iCycler iQ Detection System for TaqMan Assays, Rev B
8. Multiplexed Quantitative Peptide Assays for Protein Biomarkers of Cardiovascular Disease in Human Plasma
9. Caspase-3 Activation - An Indicator of Apoptosis in Image-Based Assays
10. Amplification Efficiency of TaqMan Gene Expression Assays
11. Cell Proliferation Assays
Post Your Comments:
(Date:1/15/2014)... Sunnyvale, CA (PRWEB) January 15, 2014 ... throughput research solutions, today announced that Lupin Limited, one ... of Freeslate’s CM Protégé PharmD System for ... in Mumbai, India, is focused on a wide range ...
(Date:1/15/2014)... , Jan. 15, 2014 TaiGen Biotechnology Company, Limited ... agreement with R-Pharm, a leading Russian pharmaceutical company, to ... Russian Federation , Turkey ... States (CIS). Nemonoxacin is a novel antibiotic for the treatment ...
(Date:1/14/2014)... Carahsoft and CDS Federal Services have scheduled a ... EST (11am PST), “Natural Language Processing: Converting Raw Data ... can turn raw, heterogeneous data into actionable knowledge to ... webinar will last approximately one hour. , Synopsis: Big ...
(Date:1/14/2014)... WASHINGTON , Jan. 14, 2014  3D Communications, a leading provider ... scientific, regulatory, business, and media events in the United ... its former associate Virginia Cox , JD, is returning to ... Virginia Cox re-joins 3D after more than two years ...
Breaking Biology Technology:Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 2Lupin Selects Freeslate’s CM Protégé PharmD System to Accelerate Polymorph Screening for Drug Development 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 2TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 3TaiGen Biotechnology Signed Exclusive License Agreement with R-Pharm for Nemonoxacin (Taigexyn(R)) 4Webcast - Natural Language Processing: Converting Raw Data into Actionable Knowledge – Hosted by Carahsoft and CDS Federal Services 2Former FDA Associate Commissioner Returns To 3D Communications 2
... on risky sources of foreign oil combined with the ... our own storm ravaged infrastructure requires bold leadership by ... the use of corn based ethanol, biodiesel, cellulosic ethanol ... ,Everyday in Wisconsin over $15 million dollars leaves the ...
... mission statement, consider this pledge from the bipartisan Wisconsin School ... dollars on top of current dollars, but propose a complete ... the (K-12 public school) system, and then any additional dollars ... , [Tom Still is a member of the initiative] ...
... TDS Telecom's James Barr III, 65, is retiring as president and ... years in the role, the company said on Tuesday. , ,Barr's ... of TDS Telecom's local telecommunications operations, has been named chief operating ... , ,Wittwer has been with the company for two decades ...
Cached Biology Technology:Wisconsin can lead the nation to energy independence 2Wisconsin can lead the nation to energy independence 3Measuring what works and what doesn't in the prototypical school 2Measuring what works and what doesn't in the prototypical school 3
(Date:4/17/2014)... One day about eight years ago, Katia ... Riverside, and her father were on a field trip in ... an orchid they had never seen before. , Unable to ... orchids. The orchid turned out to be an unnamed ... Lophiaris silverarum . , "Lophiaris" is the genus name, comprising ...
(Date:4/17/2014)... that devastate honeybees in Europe, Asia and the United ... appear to be impacting native honeybee populations at this ... invasive pests include including Nosema microsporidia and ... to be resilient to these invasive pests, which suggests ... in Europe, Asia and the United States currently are ...
(Date:4/17/2014)... channels are implicated in many serious conditions such as ... potential target for drug therapies. Unfortunately, there is still ... Cambridge research provides fresh and unexpected insight into the ... components - β-subunit molecules - which are responsible for ... published in the most recent edition of the ...
Breaking Biology News(10 mins):Orchid named after UC Riverside researcher 2Orchid named after UC Riverside researcher 3East African honeybees are safe from invasive pests… for now 2East African honeybees are safe from invasive pests… for now 3Structure of sodium channels different than previously believed 2
... University researchers are the first to design a bioactive nanomaterial ... without the use of expensive growth factors. Minimally invasive, the ... cartilage. No conventional therapy can do this. The results ... the Proceedings of the National Academy of Sciences ...
... The Springer journal Analytical and Bioanalytical Chemistry ... its Best Paper Award 2009. Frimat is the lead ... for cell patterning. The award, accompanied by 1,000 euros, ... their research careers. The ABC Best Paper Award has ...
... known that high blood sugar levels from diabetes damage blood ... how. Now a Michigan State University scientist has discovered the ... to new treatments that halt the damage. Diabetic retinopathy ... cause of blindness in young adults in the United States. ...
Cached Biology News:Growing cartilage -- no easy task 2New bioanalytical methods have potential for investigative and screening applications 2MSU researcher identifies cell mechanism leading to diabetic blindness 2
... Cellular uptake of long double stranded RNA ... interference in a diverse group of organisms ... RNA interference leads to the inhibition of ... of the target messenger RNA (mRNA). Attempts ...
... is an easy-to-use siRNA transfection reagent that ... genes in a variety of cell lines. ... an optimized, straightforward protocol. High levels of ... following transfection of 20 to 100 nM ...
... double stranded RNA (dsRNA) has been shown ... group of organisms as well as insect ... the inhibition of protein expression by utilizing ... RNA (mRNA). Attempts to induce RNA interference ...
... distinguish collagen from muscle in tissue specimens ... stain is often used to differentiate between ... an increase in collagenous tissue. With the ... red, collagen - blue, fibrin - pink, ...
Biology Products: