For highly flexible, economical RNAi screening
FlexiPlate siRNA is a giant leap forward for more flexible RNAi screening. FlexiPlate siRNA can be ordered at 0.1 nmol, 0.25 nmol, and 1 nmol scales in 96-well plates for your choice of target genes. For maximum flexibility, select siRNAs and specify plate layout at the GeneGlobe Web portal.
Advantages of FlexiPlate siRNA:
Maximum flexibility select siRNAs targeting human and mouse genes, controls, scales, and plate layout
Economical screening screen more target genes within budget
Fast and easy access simply visit www.qiagen.com/GeneGlobe to create and save plates
Cutting-edge siRNA design HP OnGuard siRNA Design minimizes the risk of off-target effects and maximizes knockdown potency
Rapid delivery enabling screening without delay
Maximum flexibility and economical options
FlexiPlate siRNA allows design of RNAi screening experiments to suit specific requirements. siRNAs for any human or mouse gene can be selected as well as any positive and negative controls required. HP GenomeWide siRNAs, for human or mouse genes or HP Validated siRNAs, which have been shown by real-time RT-PCR to provide at least 70% knockdown, can be selected. With the user-friendly Web interface, siRNAs and controls can be placed in any of the wells of a 96-well plate or plate layout can be selected from a range of predefined layout patterns (Figure 1). siRNAs are provided in 0.1 nmol, 0.25 nmol, or 1 nmol scales, enabling economical screening us ing low or higher siRNA amounts, as required.
At QIAGENs GeneGlobe Web portal, it is easy to search for siRNAs for genes of interest and to arrange plate layouts to suit screening experiments. Lists of gene names, Entrez Gene IDs, RefSeq IDs, siRNA names, or catalog numbers can be uploaded, making the process of plate ordering fast and easy. Plates can be ordered immediately or saved for later changes or ordering. Plate layouts can also be downloaded for your records (Figure 1).
Easily Create and Order FlexiPlate siRNA
Figure 1 Create plates at www.qiagen.com/GeneGlobe
Cutting-edge siRNA design
New advances in the siRNA design process ensure that QIAGENs highly innovative and sophisticated HP OnGuard siRNA Design delivers potent and specific siRNA. siRNAs are designed using neural-network technology based on an extremely large set of data from RNAi experiments (1). siRNA design is then checked for homology to all other sequences of the genome using an up-to-date, nonredundant sequence database and a proprietary homology analysis tool which incorporates exciting new features (Figure 2).
New design features:
siRNA target sequence from up-to-date NCBI databases
siRNAs are designed against the most up-to-date gene sequences from the NCBI. Using the latest sequence information ensures accurate design and potent knockdown.
3' UTR/seed region analysis
Several studies have shown that off-target effects are frequently caused by matches of the seed region of the siR NA antisense strand with the 3' untranslated region of mRNA (2). siRNA designs are analyzed for 3' UTR/seed region complementarity and siRNAs with the least potential to cause off-target effects are selected.
siRNA design is optimized to avoid all known single nucleotide polymorphisms (SNPs) using the RefSNP database. This increases siRNA potency, as an siRNA spanning a SNP will vary in its effectiveness.
Interferon motif avoidance
siRNAs are screened for multiple sequence motifs known to result in an interferon response (3, 4). siRNAs with such motifs are rejected.
The complete solution for high-throughput RNAi
In addition to FlexiPlate siRNA, QIAGEN offers a comprehensive range of solutions for high-throughput RNAi. HiPerFect Transfection Reagent provides exceptionally high transfection efficiency using low siRNA concentrations, minimizing the risk of off-target effects and reducing costs, as less siRNA is used.
Rapid mRNA purification from large numbers of cell samples can be easily achieved using TurboCapture mRNA Kits which are ideal for high-throughput screening.
QuantiTect Primer Assays are ready-to-use primer sets for quantitative, real-time RT-PCR using SYBR Green based detection. They are available for every human and mouse gene. When used in combination with QuantiTect SYBR Green Kits, accurate and sensitive real-time RT-PCR is assured. Contact your QIAGEN sales representative or visit www.qiagen.com/siRNA for more information on these and other products for high-throughput RNAi to suit your needs.
1. Huesken, D. et al. (2005) Design of a genome-wide siRNA library using an artificial neural network. Nat. Biotechnol. 23, 995.
2. Farh, K.K. et al. (2005) The widespread impact of mammalian MicroRNAs on mRNA repression and evolution. Science 310, 1817.
3. Judge, A.D., Sood, V., Shaw, J.R., Fang, D., McClintock, K., and MacLachlan, I. (2005) Sequence-dependent stimulation of the mammalian innate immune response by synthetic siRNA. Nat Biotechnol. 23, 457.
4. Hornung, V. et al. (2005) Sequence-specific potent induction of IFN-alpha by short interfering RNA in plasmacytoid dendritic cells through TLR7. Nat Med. 11, 263.
Experience maximum flexibility at www.qiagen.com/GeneGlobe !
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