Navigation Links
Five Ways to Produce siRNAs

t require large amounts of a defined ultrapure siRNA sequence

Not suitable for:
Long term studies


Ambion's Solution: Custom, Pre-designed and Validated siRNAs
Custom siRNAs: Ambion provides premium quality, made-to-order siRNAs with your choice of synthesis options. Our standard purification procedure includes deprotection and column purification, and typically yields siRNA that is 90% pure (guaranteed >80% pure). HPLC and PAGE purification is also available, yielding siRNAs that are guaranteed >97% pure. All RNA oligonucleotides are assessed by MALDI-TOF (matrix-assisted laser desorptionionization time-of-flight) mass spectrometry and annealed siRNAs are analyzed by nondenaturing gel or capillary electrophoresis to confirm that the strands are annealed properly.

Pre-designed siRNAs: A design algorithm developed by Ambion's partner, Cenix BioScience, predicts potent and specific siRNA sequences with an impressive success rate (see Designing a Better siRNA for more details). Multiple siRNA designs are available for the human, mouse, and rat genes listed in the public RefSeq database maintained by NCBI. We can also use the algorithm to design siRNAs to other organisms or genes not in the RefSeq database.
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

Related biology technology :

1. Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production
2. Silencer Validated siRNAs
3. Cenix-Designed siRNAs for 95% of Human and Mouse Genomes
4. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
5. IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!
6. Optimizing Chemical Transfection and Electroporation of siRNAs
7. Delivering siRNAs to Difficult Cell Types
8. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
9. Deliver siRNAs Into Primary Cells
10. Optimize Transfection of siRNAs for RNAi
11. RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Five Ways Produce siRNAs

(Date:11/27/2014)... The Europe Thermal Protective Clothing ... provide crucial revenue forecasts. It estimates the market ... a CAGR of 7.5%, from 2013 to 2018. ... Thermal Protective Clothing market report for a glimpse ... by various tables and figures. , http://www.micromarketmonitor.com/market/europe-thermal-protective-clothing-5957050557.html ...
(Date:11/27/2014)... 2014 Calcivis, a medical ... tooth decay, today announces that it has completed ... Activity Imaging System. The Calcivis Caries ... and consumable combination designed to transform the assessment ... involves a unique, proprietary bioluminescence approach combined with ...
(Date:11/26/2014)... 26, 2014 2014 Deep Research ... professional and in-depth research report on the Ammonium ... its definition, classification, application, and industry chain structure ... the international market analysis, including China’s domestic market ... Europe, Asia, China, Japan etc. regions) industry analysis ...
(Date:11/26/2014)... HOUSTON and PHILADELPHIA , ... the most demanding and unforgiving environments. Human exploration of ... cognitive performance to ensure mission safety and success, and ... cognitive performance for mission success, little is known about ... aspects of cognition are primarily affected. Now, ...
Breaking Biology Technology:The Europe Thermal Protective Clothing market is estimated to grow up to $577.9 million by 2018 - Report by MicroMarket Monitor 2The Europe Thermal Protective Clothing market is estimated to grow up to $577.9 million by 2018 - Report by MicroMarket Monitor 3Calcivis Completes Clinical Study of Novel Caries Activity Imaging System 2Calcivis Completes Clinical Study of Novel Caries Activity Imaging System 3Ammonium Bifluoride Market International Analysis of Manufacturing, Technology & Trends Now at ReportsnReports.com 2Ammonium Bifluoride Market International Analysis of Manufacturing, Technology & Trends Now at ReportsnReports.com 3Penn Medicine Team Develops Cognitive Test Battery to Assess the Impact of Long Duration Spaceflights on Astronauts' Brain Function 2Penn Medicine Team Develops Cognitive Test Battery to Assess the Impact of Long Duration Spaceflights on Astronauts' Brain Function 3Penn Medicine Team Develops Cognitive Test Battery to Assess the Impact of Long Duration Spaceflights on Astronauts' Brain Function 4
... Fla. , Jan. 15 Andrew P. ... Foundation and diabetes advocate announced today that he will be ... a biotechnology company that has developed and patented a family ... and cosmetic industries. , "I,m really excited about their ...
... , Jan. 14 /PRNewswire-FirstCall/ - Medical International Technology Inc. ... that it has received clearance by FINRA to be traded on ... posted for trading on the OTC Bulletin Board at the opening ... MDLH.OB. , This is very positive news for all existing and ...
... ... Scholarships. The first is a two-year scholarship to be awarded to a graduating ... sponsor four scholarships for community-active, graduating Lake Braddock High School seniors. , ... Manassas, VA (PRWEB) January 13, ...
Cached Biology Technology:Andrew P. Mandell - Mr. Diabetes(R) to Sit On ProtoKinetix Advisory Board 2MIT is pleased to announce that it has received clearance by FINRA to be listed on the OTC Bulletin Board "MDLH.OB" 2RiVIdium Inc. Creates Five Local Scholarships 2
(Date:11/18/2014)... , Nov. 18, 2014  The Secure Identity ... Association today jointly announce the formation of The ... of its Identity and Biometric Entry and ... Framework received official support from BORDERPOL, the international ... improve and provide expertise regarding border security, traveler ...
(Date:11/18/2014)... Nov. 18, 2014   EyeLock Inc. , ... and MorphoTrust USA (Safran), a leading ... announced a strategic partnership to offer comprehensive biometric ... administration (MVA), airport screening and financial services markets. ... MorphoTrust serves consumers through a nationwide network of ...
(Date:11/11/2014)...  Forensicon, Inc., a Chicago -based ... the promotion of Yaniv Schiff from Senior ... Schiff,s new role as Director, he will lead company ... examiners and provide leadership within the company,s digital forensics ... Schiff joined Forensicon in 2006 ...
Breaking Biology News(10 mins):Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 2Identity Management, Document Authentication and Biometric Companies Form "The Airport Entry and Exit Working Group", Releasing the Framework to Help Guide the Department of Homeland Security and Congress Towards Solutions 3EyeLock, MorphoTrust Form Strategic Partnership to Pursue Commercial Enterprise Deployments 2EyeLock, MorphoTrust Form Strategic Partnership to Pursue Commercial Enterprise Deployments 3Yaniv Schiff Promoted to Director of Digital Forensics at Forensicon, Inc. 2
... and the Montreal Neurological Institute (MNI), McGill University have ... survival also play an active and major role in ... published this week in The Journal of Neuroscience ... variety of acute and chronic neurological disorders, including glaucoma ...
... impact on them caused by humanity,s carbon emissions. ... used coral from the Great Barrier Reef to reveal ... due to human activity. Professor Malcolm McCulloch, a ... Reef Studies and Australian National University today receives Australia,s ...
... (Nasdaq: AWRE ), a leading supplier of broadband ... first quarter ended March 31, 2009. Revenues for the ... 22% compared to $5.9 million in the same quarter last ... diluted net income per share in accordance with U.S. generally ...
Cached Biology News:Cell's split personality is a major discovery into neurological diseases 2Coral 'can't escape the heat' 2Aware, Inc. Reports First Quarter 2009 Financial Results 2Aware, Inc. Reports First Quarter 2009 Financial Results 3Aware, Inc. Reports First Quarter 2009 Financial Results 4Aware, Inc. Reports First Quarter 2009 Financial Results 5Aware, Inc. Reports First Quarter 2009 Financial Results 6
Human PDGF, CF...
... The mycobacteria in ... cells to the injection site which enhances ... Freund's Adjuvant is used for the initial ... the subsequent boosts. Antigens (preferably in saline) ...
2,4-Dinitrophenyl hapten is conjugated to Keyhole Limpet (KLH) protein through lysine....
IC50: 3 ng/ml · Limit of detection: 0.5 ng/ml...
Biology Products: