Navigation Links
Five Ways to Produce siRNAs

t require large amounts of a defined ultrapure siRNA sequence

Not suitable for:
Long term studies


Ambion's Solution: Custom, Pre-designed and Validated siRNAs
Custom siRNAs: Ambion provides premium quality, made-to-order siRNAs with your choice of synthesis options. Our standard purification procedure includes deprotection and column purification, and typically yields siRNA that is 90% pure (guaranteed >80% pure). HPLC and PAGE purification is also available, yielding siRNAs that are guaranteed >97% pure. All RNA oligonucleotides are assessed by MALDI-TOF (matrix-assisted laser desorptionionization time-of-flight) mass spectrometry and annealed siRNAs are analyzed by nondenaturing gel or capillary electrophoresis to confirm that the strands are annealed properly.

Pre-designed siRNAs: A design algorithm developed by Ambion's partner, Cenix BioScience, predicts potent and specific siRNA sequences with an impressive success rate (see Designing a Better siRNA for more details). Multiple siRNA designs are available for the human, mouse, and rat genes listed in the public RefSeq database maintained by NCBI. We can also use the algorithm to design siRNAs to other organisms or genes not in the RefSeq database.
'"/>

Source:


Page: All 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20

Related biology technology :

1. Gram Quantities of MAb Produced with Simple Bioreactor in Serum-Free Perfusion Culture Replacing Ascitic Fluid Production
2. Silencer Validated siRNAs
3. Cenix-Designed siRNAs for 95% of Human and Mouse Genomes
4. Efficient Delivery of siRNAs to Human Primary Cells: Electroporation vs. Chemical Transfection
5. IMPROVE SILENCING SUCCESS: siRNAs THAT WORK BETTER!
6. Optimizing Chemical Transfection and Electroporation of siRNAs
7. Delivering siRNAs to Difficult Cell Types
8. Matched siRNAs and Assays: Ambion + Applied Biosystems = RNAi Success
9. Deliver siRNAs Into Primary Cells
10. Optimize Transfection of siRNAs for RNAi
11. RNAi as a Tool for Mammalian Gene Analysis: Applications of siRNAs
Post Your Comments:
*Name:
*Comment:
*Email:
TAG: Five Ways Produce siRNAs

(Date:7/10/2014)... (PRWEB) July 10, 2014 Women ... have access to an additional family planning option. ... to contraception at all levels of the health ... formulation of a widely used contraceptive—Pfizer’s Depo-Provera® (depot ... Burkina Faso is the first of four African ...
(Date:7/10/2014)... Francisco, CA (PRWEB) July 10, 2014 ... family closer together. The Archer Family purchased a ... contained within DNA, but ended up with a lifetime ... discoveries contained within the family members’ DNA genuinely brought ... had decided to search deeper into genetic history and ...
(Date:7/10/2014)... , July 10, 2014  Franciscan St. ... of capnography for respiratory monitoring outside the ... healthcare leaders in embracing state-of-the-art patient safety ... patients are breathing and can alert medical ... measuring the amount of carbon dioxide the ...
(Date:7/10/2014)... July 10, 2014   Ceres, Inc . (Nasdaq: ... company, announced today financial results for the three months ... its business. Ceres reported that the company ... performance this growing season in Brazil, which concluded in ... company,s sorghum evaluation areas for part of the growing ...
Breaking Biology Technology:Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 2Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 3Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 4Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 5Injectable Contraceptive Launched in Burkina Faso to Expand Choice and Address Unmet Need 6Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 2Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 3Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 4Ancestry DNA Test Reveals a Controversial and Undiscovered Family History 5Franciscan St. Anthony Health-Crown Point Underscores Patient Safety Commitment Through Expanded Use of Capnography 2Ceres Announces Fiscal Third Quarter 2014 Financial Results 2Ceres Announces Fiscal Third Quarter 2014 Financial Results 3Ceres Announces Fiscal Third Quarter 2014 Financial Results 4Ceres Announces Fiscal Third Quarter 2014 Financial Results 5Ceres Announces Fiscal Third Quarter 2014 Financial Results 6Ceres Announces Fiscal Third Quarter 2014 Financial Results 7Ceres Announces Fiscal Third Quarter 2014 Financial Results 8Ceres Announces Fiscal Third Quarter 2014 Financial Results 9
... the Harvard Business Review (HBR), containing an ... , questioned the impact of IT investments. This has ... One of the dissenters of Carrs article is PRISM ... specializes in Outsourced Marketing Services and uses technology to ...
... eFilm to offer an integrated PAC (picture achieving and ... target market of hospitals and imaging centers. , ... milestone in our evolution as a healthcare software and ... our target market," said Rich Linden, Merge eFilm president ...
... Ill. Though electricity and telephones dont even exist at ... the next highly underserved segment to be wired by ... Monday. Motorola says Indian reservations could be the most ... ,When identifying the need for high-speed Internet access for ...
Cached Biology Technology:IT Does Matter! 2IT Does Matter! 3Merge eFilm to buy Ohio firm in $14M Deal 2Merge eFilm to buy Ohio firm in $14M Deal 3Motorola Canopy Wires Indian Reservations 2
(Date:7/11/2014)... Researchers have pioneered a revolutionary new way to ... Virtual Finger, allows scientists to move through digital ... using the flat surface of their computer screens. ... orders of magnitude more efficient, saving time, money ... areas of experimental biology. The software and its ...
(Date:7/11/2014)... to the hippocampus, the marginal division of the ... What is the impact degree of substance P ... memory function? Yan Yu and his team, College ... using immunofluorescence staining, that substance P receptor, neurokinin ... striatal marginal division of normal rats. Unilateral or ...
(Date:7/11/2014)... 11, 2014 Research and Markets ... Technology Market - Industry Analysis Size Share Growth Trends ... http://photos.prnewswire.com/prnh/20130307/600769 ... created a need for high level security in both ... authentication such as username and passwords, tokens etc. are ...
Breaking Biology News(10 mins):Virtual finger enables scientists to navigate and analyze complex 3D images 2International Biometrics Technology Market - Industry Analysis Size Share Growth Trends and Forecast to 2019 2
... 8, 2011 When expressed (turned on), the ... the enzyme telomerase. Human cells can keep living ... i.e. the cells become immortal . Telomerase ... extend human cell life indefinitely. TA-65®  is a ...
... , NARRAGANSETT, RI -- June 7, 2011 -- Sunshine Menezes, ... Reporting, and 2010 Grantham Prize winner Alanna Mitchell announced the ... on the Environment. James Astill of The Economist will receive ... Them," a commanding 8-part special report on the state of ...
... only organisms that turn to caffeine for a pick-me-up. University ... can live on caffeine. One of them, known as ... outside a UI research laboratory. The research team says the ... works -- could in the future allow scientists to convert ...
Cached Biology News:A Molecule That Turns on the Immortality Gene! 2A Molecule That Turns on the Immortality Gene! 3$75,000 Grantham Prize winner announced 2$75,000 Grantham Prize winner announced 3University of Iowa researchers identify caffeine-consuming bacterium 2
Monoclonal Mouse Anti-Human Cytokeratin 16 (48 kD)...
Sheep polyclonal to Melatonin ( Abpromise for all tested applications). Antigen: Melatonin Tg conjugate....
Rabbit polyclonal to NCoR2, prediluted ( Abpromise for all tested applications). entrezGeneID: 9612 SwissProtID: Q9Y618...
Rabbit polyclonal to TRPM8 (rating: ****) ( Abpromise for all tested applications). entrezGeneID: 79054 SwissProtID: Q3YFM7...
Biology Products: